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Low EphA7 Expression Correlated with Lymph Node Metastasis and Poor Prognosis of Patients with Esophageal Squamous Cell Carcinoma.

Bai YQ, Zhang JY, Bai CY, Xu XE, Wu JY, Chen B, Wu ZY, Wang SH, Shen J, Shen JH, Yao XD, Gao LZ, Wu B, Gu HL, Liu XH, Li X, Li EM, Xu LY - Acta Histochem Cytochem (2015)

Bottom Line: As a member of the Eph family of receptor tyrosine kinases, EphA7 plays an important role in cancer.The results showed that low EphA7 expression significantly correlated with lymph node metastases (N0: 29%; N1: 64%. p<0.001), poor degree of tumor differentiation (G1: 31%; G2: 49%; G3: 58%. p=0.009) and pTNM staging (I+II: 33%; III+IV: 58%. p<0.001).Furthermore, in a combined analysis, patients with low EphA7-expressing tumors showed a shorter overall survival than those with high expression, resulting in a five-year overall survival rate of 47.4% vs. 52.6%, respectively (p=0.016).

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Medical College of Chifeng University ; The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College.

ABSTRACT
As a member of the Eph family of receptor tyrosine kinases, EphA7 plays an important role in cancer. However, the expression and significance of Eph receptors in esophageal squamous cell carcinoma (ESCC) remain unclear. Here, we detected the expression of EphA7 by immunohistochemistry in a sample of 352 patients with ESCC, and aimed to investigate the expression status of EphA7 in ESCC and its impact on prognosis. The results showed that low EphA7 expression significantly correlated with lymph node metastases (N0: 29%; N1: 64%. p<0.001), poor degree of tumor differentiation (G1: 31%; G2: 49%; G3: 58%. p=0.009) and pTNM staging (I+II: 33%; III+IV: 58%. p<0.001). Furthermore, in a combined analysis, patients with low EphA7-expressing tumors showed a shorter overall survival than those with high expression, resulting in a five-year overall survival rate of 47.4% vs. 52.6%, respectively (p=0.016). Consequently, patients with a low EphA7 expression have poorer prognosis in ESCC compared with those manifesting high expression.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical analysis of EphA7 expression in the progression from normal esophageal mucosa (a, b), low-grade (c, d), to high-grade (e, f) dysplasia. EphA7 located in the cytoplasm and cell membrane of the differentiated zones (a and b). Immunostaining of EphA7 was also seen in the cytoplasm and cell membrane of low-grade (c and d) and high-grade dysplasia (e and f) but EphA7 was negative in the cytoplasm and cell membrane of high-grade dysplasia involving atypical cell layer (e and f). Bars=200 μm.
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Figure 1: Immunohistochemical analysis of EphA7 expression in the progression from normal esophageal mucosa (a, b), low-grade (c, d), to high-grade (e, f) dysplasia. EphA7 located in the cytoplasm and cell membrane of the differentiated zones (a and b). Immunostaining of EphA7 was also seen in the cytoplasm and cell membrane of low-grade (c and d) and high-grade dysplasia (e and f) but EphA7 was negative in the cytoplasm and cell membrane of high-grade dysplasia involving atypical cell layer (e and f). Bars=200 μm.

Mentions: EphA7 expression in ESCC was investigated by immunohistochemical analysis of formalin-fixed, paraffin-embedded specimens using an EphA7-specific MAb. In normal esophageal tissue, EphA7 immunostaining appeared in the cytoplasm and cell membrane of the differentiated zones (Fig. 1a and b). Immunostaining of EphA7 was also seen in the cytoplasm and cell membrane of low-grade and high-grade dysplasia, particularly in cells located in the differentiated zones (Fig. 1c–f). However, the cytoplasm and cell membrane of the atypical cell layer cells in the high-grade dysplasia were EphA7-negative (Fig. 1e and f). In addition, weak, moderate or strong immunostaining of EphA7 was found in the cytoplasm of cancer cell nests with abundant keratin pearl cells (Fig. 2b–d). However, complete loss of EphA7 expression was found in the cytoplasm of cancer cell nests without formation of abundant keratin pearl cells (Fig. 2a). These data indicate that EphA7 expression may be related to tumor differentiation.


Low EphA7 Expression Correlated with Lymph Node Metastasis and Poor Prognosis of Patients with Esophageal Squamous Cell Carcinoma.

Bai YQ, Zhang JY, Bai CY, Xu XE, Wu JY, Chen B, Wu ZY, Wang SH, Shen J, Shen JH, Yao XD, Gao LZ, Wu B, Gu HL, Liu XH, Li X, Li EM, Xu LY - Acta Histochem Cytochem (2015)

Immunohistochemical analysis of EphA7 expression in the progression from normal esophageal mucosa (a, b), low-grade (c, d), to high-grade (e, f) dysplasia. EphA7 located in the cytoplasm and cell membrane of the differentiated zones (a and b). Immunostaining of EphA7 was also seen in the cytoplasm and cell membrane of low-grade (c and d) and high-grade dysplasia (e and f) but EphA7 was negative in the cytoplasm and cell membrane of high-grade dysplasia involving atypical cell layer (e and f). Bars=200 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4491497&req=5

Figure 1: Immunohistochemical analysis of EphA7 expression in the progression from normal esophageal mucosa (a, b), low-grade (c, d), to high-grade (e, f) dysplasia. EphA7 located in the cytoplasm and cell membrane of the differentiated zones (a and b). Immunostaining of EphA7 was also seen in the cytoplasm and cell membrane of low-grade (c and d) and high-grade dysplasia (e and f) but EphA7 was negative in the cytoplasm and cell membrane of high-grade dysplasia involving atypical cell layer (e and f). Bars=200 μm.
Mentions: EphA7 expression in ESCC was investigated by immunohistochemical analysis of formalin-fixed, paraffin-embedded specimens using an EphA7-specific MAb. In normal esophageal tissue, EphA7 immunostaining appeared in the cytoplasm and cell membrane of the differentiated zones (Fig. 1a and b). Immunostaining of EphA7 was also seen in the cytoplasm and cell membrane of low-grade and high-grade dysplasia, particularly in cells located in the differentiated zones (Fig. 1c–f). However, the cytoplasm and cell membrane of the atypical cell layer cells in the high-grade dysplasia were EphA7-negative (Fig. 1e and f). In addition, weak, moderate or strong immunostaining of EphA7 was found in the cytoplasm of cancer cell nests with abundant keratin pearl cells (Fig. 2b–d). However, complete loss of EphA7 expression was found in the cytoplasm of cancer cell nests without formation of abundant keratin pearl cells (Fig. 2a). These data indicate that EphA7 expression may be related to tumor differentiation.

Bottom Line: As a member of the Eph family of receptor tyrosine kinases, EphA7 plays an important role in cancer.The results showed that low EphA7 expression significantly correlated with lymph node metastases (N0: 29%; N1: 64%. p<0.001), poor degree of tumor differentiation (G1: 31%; G2: 49%; G3: 58%. p=0.009) and pTNM staging (I+II: 33%; III+IV: 58%. p<0.001).Furthermore, in a combined analysis, patients with low EphA7-expressing tumors showed a shorter overall survival than those with high expression, resulting in a five-year overall survival rate of 47.4% vs. 52.6%, respectively (p=0.016).

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Medical College of Chifeng University ; The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University Medical College.

ABSTRACT
As a member of the Eph family of receptor tyrosine kinases, EphA7 plays an important role in cancer. However, the expression and significance of Eph receptors in esophageal squamous cell carcinoma (ESCC) remain unclear. Here, we detected the expression of EphA7 by immunohistochemistry in a sample of 352 patients with ESCC, and aimed to investigate the expression status of EphA7 in ESCC and its impact on prognosis. The results showed that low EphA7 expression significantly correlated with lymph node metastases (N0: 29%; N1: 64%. p<0.001), poor degree of tumor differentiation (G1: 31%; G2: 49%; G3: 58%. p=0.009) and pTNM staging (I+II: 33%; III+IV: 58%. p<0.001). Furthermore, in a combined analysis, patients with low EphA7-expressing tumors showed a shorter overall survival than those with high expression, resulting in a five-year overall survival rate of 47.4% vs. 52.6%, respectively (p=0.016). Consequently, patients with a low EphA7 expression have poorer prognosis in ESCC compared with those manifesting high expression.

No MeSH data available.


Related in: MedlinePlus