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DC-SIGN (CD209) Carbohydrate Recognition Domain Is Not Polymorphic in Dengue Virus-Infected Indonesian Patients.

Wibawa T, Wijayanti N, Arguni E, Laksono IS - Trop Med Health (2015)

Bottom Line: The aim of this work is to measure the frequency of these polymorphisms among asymptomatic and hospitalized DENV-infected patients.Among the subjects, we performed PCR amplification and DNA direct seqencing for 23 hospitalized DENV-infected patients and 24 asymptomatic DENV-infected patients.The result showed that there were no polymorphic nucleotides in the CD209 encoded gene among the patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Faculty of Medicine, Universitas Gadjah Mada, Indonesia.

ABSTRACT
Dengue virus (DENV) infection is a significant burden in Indonesia and other tropical countries. DENV infection has a wide spectrum of clinical manifestations, i.e. asymptomatic, dengue fever, dengue hemorrhagic fever and dengue shock syndrome. The variety of clinical manifestations may be due to the diversity of genetic constitution of the host. The C-type lectin DC-SIGN (CD209) has been identified as the major dengue receptor on human dendritic cells. There are at least five polymorphisms in exon 5 and 6 of the DC-SIGN encoded gene which have been identified and recorded in dbSNP. The aim of this work is to measure the frequency of these polymorphisms among asymptomatic and hospitalized DENV-infected patients. We enrolled 23 hospitalized and 73 asymptomatic DENV-infected patients. Among the subjects, we performed PCR amplification and DNA direct seqencing for 23 hospitalized DENV-infected patients and 24 asymptomatic DENV-infected patients. The result showed that there were no polymorphic nucleotides in the CD209 encoded gene among the patients.

No MeSH data available.


Related in: MedlinePlus

DNA Sequencing of DC-SIGN (CD209) carbohydrate recognition domain (CRD) exon 5 and 6. Arrows denote the location of SNPs previously reported in the dbSNPs database: namely: rs138694095 (GAT→GGT), rs11465393 (GCC→TCC); rs148485547 (TCA→TAA); rs146002807 (ACG→ATG); and rs75008691 (CGC→CAC). The SNPs were not identified in Indonesian DENV-infected patients.
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Related In: Results  -  Collection


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Figure 2: DNA Sequencing of DC-SIGN (CD209) carbohydrate recognition domain (CRD) exon 5 and 6. Arrows denote the location of SNPs previously reported in the dbSNPs database: namely: rs138694095 (GAT→GGT), rs11465393 (GCC→TCC); rs148485547 (TCA→TAA); rs146002807 (ACG→ATG); and rs75008691 (CGC→CAC). The SNPs were not identified in Indonesian DENV-infected patients.

Mentions: The regions corresponding to the locus previously reported as polymorphic were: rs138694095 (GAT→GGT), rs11465393 (GCC→TCC); rs148485547 (TCA→TAA); rs146002807 (ACG→ATG); and rs75008691 (CGC→CAC), were sequenced (Fig. 2). The results showed that there was no polymophism among the two groups.


DC-SIGN (CD209) Carbohydrate Recognition Domain Is Not Polymorphic in Dengue Virus-Infected Indonesian Patients.

Wibawa T, Wijayanti N, Arguni E, Laksono IS - Trop Med Health (2015)

DNA Sequencing of DC-SIGN (CD209) carbohydrate recognition domain (CRD) exon 5 and 6. Arrows denote the location of SNPs previously reported in the dbSNPs database: namely: rs138694095 (GAT→GGT), rs11465393 (GCC→TCC); rs148485547 (TCA→TAA); rs146002807 (ACG→ATG); and rs75008691 (CGC→CAC). The SNPs were not identified in Indonesian DENV-infected patients.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4491492&req=5

Figure 2: DNA Sequencing of DC-SIGN (CD209) carbohydrate recognition domain (CRD) exon 5 and 6. Arrows denote the location of SNPs previously reported in the dbSNPs database: namely: rs138694095 (GAT→GGT), rs11465393 (GCC→TCC); rs148485547 (TCA→TAA); rs146002807 (ACG→ATG); and rs75008691 (CGC→CAC). The SNPs were not identified in Indonesian DENV-infected patients.
Mentions: The regions corresponding to the locus previously reported as polymorphic were: rs138694095 (GAT→GGT), rs11465393 (GCC→TCC); rs148485547 (TCA→TAA); rs146002807 (ACG→ATG); and rs75008691 (CGC→CAC), were sequenced (Fig. 2). The results showed that there was no polymophism among the two groups.

Bottom Line: The aim of this work is to measure the frequency of these polymorphisms among asymptomatic and hospitalized DENV-infected patients.Among the subjects, we performed PCR amplification and DNA direct seqencing for 23 hospitalized DENV-infected patients and 24 asymptomatic DENV-infected patients.The result showed that there were no polymorphic nucleotides in the CD209 encoded gene among the patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Faculty of Medicine, Universitas Gadjah Mada, Indonesia.

ABSTRACT
Dengue virus (DENV) infection is a significant burden in Indonesia and other tropical countries. DENV infection has a wide spectrum of clinical manifestations, i.e. asymptomatic, dengue fever, dengue hemorrhagic fever and dengue shock syndrome. The variety of clinical manifestations may be due to the diversity of genetic constitution of the host. The C-type lectin DC-SIGN (CD209) has been identified as the major dengue receptor on human dendritic cells. There are at least five polymorphisms in exon 5 and 6 of the DC-SIGN encoded gene which have been identified and recorded in dbSNP. The aim of this work is to measure the frequency of these polymorphisms among asymptomatic and hospitalized DENV-infected patients. We enrolled 23 hospitalized and 73 asymptomatic DENV-infected patients. Among the subjects, we performed PCR amplification and DNA direct seqencing for 23 hospitalized DENV-infected patients and 24 asymptomatic DENV-infected patients. The result showed that there were no polymorphic nucleotides in the CD209 encoded gene among the patients.

No MeSH data available.


Related in: MedlinePlus