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Evaluation of Genetic Variations in Maize Seedlings Exposed to Electric Field Based on Protein and DNA Markers.

Al-Huqail AA, Abdelhaliem E - Biomed Res Int (2015)

Bottom Line: Leucine-aminopeptidase, peroxidase, and catalase isozymes showed the highest values of polymorphism (100%) based on zymograms number, relative front (Rf ), and optical intensity while esterase isozyme generated polymorphism value of 83.33%.Amino acids were analyzed using high-performance liquid chromatography, which revealed the presence of 17 amino acids of variable contents ranging from 22.65% to 28.09%.RAPD revealed that 78 amplified DNA products had highly polymorphism value (95.08%) based on band numbers, with variable sizes ranging from 120 to 992 base pairs and band intensity.

View Article: PubMed Central - PubMed

Affiliation: Plant Physiology, Botany and Microbiology Department, Science College, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia.

ABSTRACT
The current study analyzed proteins and nuclear DNA of electric fields (ELF) exposed and nonexposed maize seedlings for different exposure periods using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), isozymes, random amplified polymorphic DNA (RAPD), and comet assay, respectively. SDS-PAGE analysis revealed total of 46 polypeptides bands with different molecular weights ranging from 186.20 to 36.00 KDa. It generated distinctive polymorphism value of 84.62%. Leucine-aminopeptidase, peroxidase, and catalase isozymes showed the highest values of polymorphism (100%) based on zymograms number, relative front (Rf ), and optical intensity while esterase isozyme generated polymorphism value of 83.33%. Amino acids were analyzed using high-performance liquid chromatography, which revealed the presence of 17 amino acids of variable contents ranging from 22.65% to 28.09%. RAPD revealed that 78 amplified DNA products had highly polymorphism value (95.08%) based on band numbers, with variable sizes ranging from 120 to 992 base pairs and band intensity. Comet assay recorded the highest extent of nuclear DNA damage as percentage of tailed DNA (2.38%) and tail moment unit (5.36) at ELF exposure of maize nuclei for 5 days. The current study concluded that the longer ELF exposing periods had genotoxic stress on macromolecules of maize cells and biomarkers used should be augmented for reliable estimates of genotoxicity after exposure of economic plants to ELF stressors.

No MeSH data available.


Related in: MedlinePlus

Banding pattern (a) and schematic distribution (b) of LAP, EST, PER, and CAT zymograms (Rf values) in ELF expose and nonexposed maize seedlings.
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fig2: Banding pattern (a) and schematic distribution (b) of LAP, EST, PER, and CAT zymograms (Rf values) in ELF expose and nonexposed maize seedlings.

Mentions: LAP, EST, PER, and CAT isozymes used in the current study showed clear polymorphisms between exposed maize seedlings to ELF treatment (T1, T3, and T5) compared to nonexposed one as shown in Tables 2–5 and Figure 2. These polymorphisms are based on the number of zymograms, the Rf values, and their optical intensities. A total of 63 different electrophoretic zymograms were observed for the 4 isozymes. LAP, EST, PER, and CAT analyses generated 13, 19, 16, and 15 zymograms, respectively, with different Rf values ranging from 0.20 to 0.89 for LAP, 0.13–0.91 for EST, 0.07–1.05 for PER, and 0.04–0.99 for CAT. LAP, EST, PER, and CAT analyses generated higher number of zymograms (4, 5, 6, and 5 with values 30.77%, 26.32%, 37.5%, and 33.33%, resp.) at treatments (T3), (T0, T1, and T3), (T5), and (T0), respectively, while lower number of zymograms (3, 4, 3, and 3 with values 23.08%, 21.05%, 18.75%, and 20.00%, resp.) was scored at treatments (T0, T1, and T3), (T5), (T0 and T3), and (T3 and T5), respectively. On the other hand, LAP analysis revealed 10 polymorphic zymograms (8 unique and 2 nonunique zymograms). These zymograms generated a high value of polymorphism of 100% while EST analysis revealed 10 polymorphic zymograms (9 unique and 1 nonunique zymograms) and 2 monomorphic zymograms with a considerable polymorphism value of 83.33%. Moreover, PER analysis revealed 13 polymorphic zymograms (10 unique and 3 nonunique zymograms) while CAT analysis revealed 12 polymorphic zymograms (10 unique and 2 nonunique zymograms). These two isozymes generated a high value of polymorphism of 100%.


Evaluation of Genetic Variations in Maize Seedlings Exposed to Electric Field Based on Protein and DNA Markers.

Al-Huqail AA, Abdelhaliem E - Biomed Res Int (2015)

Banding pattern (a) and schematic distribution (b) of LAP, EST, PER, and CAT zymograms (Rf values) in ELF expose and nonexposed maize seedlings.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4491376&req=5

fig2: Banding pattern (a) and schematic distribution (b) of LAP, EST, PER, and CAT zymograms (Rf values) in ELF expose and nonexposed maize seedlings.
Mentions: LAP, EST, PER, and CAT isozymes used in the current study showed clear polymorphisms between exposed maize seedlings to ELF treatment (T1, T3, and T5) compared to nonexposed one as shown in Tables 2–5 and Figure 2. These polymorphisms are based on the number of zymograms, the Rf values, and their optical intensities. A total of 63 different electrophoretic zymograms were observed for the 4 isozymes. LAP, EST, PER, and CAT analyses generated 13, 19, 16, and 15 zymograms, respectively, with different Rf values ranging from 0.20 to 0.89 for LAP, 0.13–0.91 for EST, 0.07–1.05 for PER, and 0.04–0.99 for CAT. LAP, EST, PER, and CAT analyses generated higher number of zymograms (4, 5, 6, and 5 with values 30.77%, 26.32%, 37.5%, and 33.33%, resp.) at treatments (T3), (T0, T1, and T3), (T5), and (T0), respectively, while lower number of zymograms (3, 4, 3, and 3 with values 23.08%, 21.05%, 18.75%, and 20.00%, resp.) was scored at treatments (T0, T1, and T3), (T5), (T0 and T3), and (T3 and T5), respectively. On the other hand, LAP analysis revealed 10 polymorphic zymograms (8 unique and 2 nonunique zymograms). These zymograms generated a high value of polymorphism of 100% while EST analysis revealed 10 polymorphic zymograms (9 unique and 1 nonunique zymograms) and 2 monomorphic zymograms with a considerable polymorphism value of 83.33%. Moreover, PER analysis revealed 13 polymorphic zymograms (10 unique and 3 nonunique zymograms) while CAT analysis revealed 12 polymorphic zymograms (10 unique and 2 nonunique zymograms). These two isozymes generated a high value of polymorphism of 100%.

Bottom Line: Leucine-aminopeptidase, peroxidase, and catalase isozymes showed the highest values of polymorphism (100%) based on zymograms number, relative front (Rf ), and optical intensity while esterase isozyme generated polymorphism value of 83.33%.Amino acids were analyzed using high-performance liquid chromatography, which revealed the presence of 17 amino acids of variable contents ranging from 22.65% to 28.09%.RAPD revealed that 78 amplified DNA products had highly polymorphism value (95.08%) based on band numbers, with variable sizes ranging from 120 to 992 base pairs and band intensity.

View Article: PubMed Central - PubMed

Affiliation: Plant Physiology, Botany and Microbiology Department, Science College, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia.

ABSTRACT
The current study analyzed proteins and nuclear DNA of electric fields (ELF) exposed and nonexposed maize seedlings for different exposure periods using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), isozymes, random amplified polymorphic DNA (RAPD), and comet assay, respectively. SDS-PAGE analysis revealed total of 46 polypeptides bands with different molecular weights ranging from 186.20 to 36.00 KDa. It generated distinctive polymorphism value of 84.62%. Leucine-aminopeptidase, peroxidase, and catalase isozymes showed the highest values of polymorphism (100%) based on zymograms number, relative front (Rf ), and optical intensity while esterase isozyme generated polymorphism value of 83.33%. Amino acids were analyzed using high-performance liquid chromatography, which revealed the presence of 17 amino acids of variable contents ranging from 22.65% to 28.09%. RAPD revealed that 78 amplified DNA products had highly polymorphism value (95.08%) based on band numbers, with variable sizes ranging from 120 to 992 base pairs and band intensity. Comet assay recorded the highest extent of nuclear DNA damage as percentage of tailed DNA (2.38%) and tail moment unit (5.36) at ELF exposure of maize nuclei for 5 days. The current study concluded that the longer ELF exposing periods had genotoxic stress on macromolecules of maize cells and biomarkers used should be augmented for reliable estimates of genotoxicity after exposure of economic plants to ELF stressors.

No MeSH data available.


Related in: MedlinePlus