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The lipopeptides pseudofactin II and surfactin effectively decrease Candida albicans adhesion and hydrophobicity.

Biniarz P, Baranowska G, Feder-Kubis J, Krasowska A - Antonie Van Leeuwenhoek (2015)

Bottom Line: When microplates were pre-coated with biosurfactants, PF II was less active than SU, but when cells were incubated together with biosurfactants, the activity of both compounds was similar, independent of the CSH of strains.This suggests irreversible changes in the cell wall after the treatment with biosurfactants.Preincubation of C. albicans with biosurfactants caused extraction of cell wall proteins with molecular mass in the range of 10-40 kDa, which is one possible mechanism of action of the tested lipopeptides.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biotechnology, University of Wrocław, ul. Fryderyka Joliot-Curie 14a, 50-383, Wrocław, Poland.

ABSTRACT
A serious problem for humans is the propensity of Candida albicans to adhere to various surfaces and its ability to form biofilms. Surfactants or biosurfactants can affect the cell surfaces of microorganisms and block their adhesion to different substrates. This study investigated adhesion of C. albicans strains differing in cell surface hydrophobicity (CSH) to polystyrene microplates in order to compare the ability of lipopeptide biosurfactants pseudofactin (PF II) and surfactin (SU) to prevent fungal adhesion to polystyrene. The biosurfactants decreased adhesion of tested strains by 35-90 % when microplates were conditioned before the addition of cells. A 80-90 % reduction of adhesion was observed when cells were incubated together with lipopeptides in microplates. When microplates were pre-coated with biosurfactants, PF II was less active than SU, but when cells were incubated together with biosurfactants, the activity of both compounds was similar, independent of the CSH of strains. When cells were preincubated with lipopeptides and then the compounds were washed out, the adhesion of hydrophobic strains increased two times in comparison to control samples. This suggests irreversible changes in the cell wall after the treatment with biosurfactants. CSH of hydrophobic strains decreased only by 20-60 % after incubation with biosurfactants while adhesion decreased by 80-90 %; the changes in cell adhesion can be thus only partially explained through the modification of CSH. Preincubation of C. albicans with biosurfactants caused extraction of cell wall proteins with molecular mass in the range of 10-40 kDa, which is one possible mechanism of action of the tested lipopeptides.

No MeSH data available.


Related in: MedlinePlus

Propidium iodine (PI) fluorescence and differential interference contrast (DIC) microphotographs of C. albicans cells incubated with 0.1 mg/ml PF II or 0.015 mg/ml SU in comparison with control samples (C) and 1 % SDS as positive controls
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Fig8: Propidium iodine (PI) fluorescence and differential interference contrast (DIC) microphotographs of C. albicans cells incubated with 0.1 mg/ml PF II or 0.015 mg/ml SU in comparison with control samples (C) and 1 % SDS as positive controls

Mentions: To exclude the possibility of contamination of cell-free supernatants (Fig. 7) with cytoplasmic proteins, we analyzed viability and membrane permeability of Candida cells with fluorescence microscopy (Fig. 8). The lack of propidium iodine (PI) fluorescence in control samples and cells incubated with lipopeptides indicate that cells were viable and membranes permeability was undisturbed (Fig. 8), which also confirms viability results shown earlier (Fig. 2). In contrast, cells treated with 1 % SDS showed significant fluorescence of dead cells.Fig. 8


The lipopeptides pseudofactin II and surfactin effectively decrease Candida albicans adhesion and hydrophobicity.

Biniarz P, Baranowska G, Feder-Kubis J, Krasowska A - Antonie Van Leeuwenhoek (2015)

Propidium iodine (PI) fluorescence and differential interference contrast (DIC) microphotographs of C. albicans cells incubated with 0.1 mg/ml PF II or 0.015 mg/ml SU in comparison with control samples (C) and 1 % SDS as positive controls
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4491367&req=5

Fig8: Propidium iodine (PI) fluorescence and differential interference contrast (DIC) microphotographs of C. albicans cells incubated with 0.1 mg/ml PF II or 0.015 mg/ml SU in comparison with control samples (C) and 1 % SDS as positive controls
Mentions: To exclude the possibility of contamination of cell-free supernatants (Fig. 7) with cytoplasmic proteins, we analyzed viability and membrane permeability of Candida cells with fluorescence microscopy (Fig. 8). The lack of propidium iodine (PI) fluorescence in control samples and cells incubated with lipopeptides indicate that cells were viable and membranes permeability was undisturbed (Fig. 8), which also confirms viability results shown earlier (Fig. 2). In contrast, cells treated with 1 % SDS showed significant fluorescence of dead cells.Fig. 8

Bottom Line: When microplates were pre-coated with biosurfactants, PF II was less active than SU, but when cells were incubated together with biosurfactants, the activity of both compounds was similar, independent of the CSH of strains.This suggests irreversible changes in the cell wall after the treatment with biosurfactants.Preincubation of C. albicans with biosurfactants caused extraction of cell wall proteins with molecular mass in the range of 10-40 kDa, which is one possible mechanism of action of the tested lipopeptides.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biotechnology, University of Wrocław, ul. Fryderyka Joliot-Curie 14a, 50-383, Wrocław, Poland.

ABSTRACT
A serious problem for humans is the propensity of Candida albicans to adhere to various surfaces and its ability to form biofilms. Surfactants or biosurfactants can affect the cell surfaces of microorganisms and block their adhesion to different substrates. This study investigated adhesion of C. albicans strains differing in cell surface hydrophobicity (CSH) to polystyrene microplates in order to compare the ability of lipopeptide biosurfactants pseudofactin (PF II) and surfactin (SU) to prevent fungal adhesion to polystyrene. The biosurfactants decreased adhesion of tested strains by 35-90 % when microplates were conditioned before the addition of cells. A 80-90 % reduction of adhesion was observed when cells were incubated together with lipopeptides in microplates. When microplates were pre-coated with biosurfactants, PF II was less active than SU, but when cells were incubated together with biosurfactants, the activity of both compounds was similar, independent of the CSH of strains. When cells were preincubated with lipopeptides and then the compounds were washed out, the adhesion of hydrophobic strains increased two times in comparison to control samples. This suggests irreversible changes in the cell wall after the treatment with biosurfactants. CSH of hydrophobic strains decreased only by 20-60 % after incubation with biosurfactants while adhesion decreased by 80-90 %; the changes in cell adhesion can be thus only partially explained through the modification of CSH. Preincubation of C. albicans with biosurfactants caused extraction of cell wall proteins with molecular mass in the range of 10-40 kDa, which is one possible mechanism of action of the tested lipopeptides.

No MeSH data available.


Related in: MedlinePlus