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The lipopeptides pseudofactin II and surfactin effectively decrease Candida albicans adhesion and hydrophobicity.

Biniarz P, Baranowska G, Feder-Kubis J, Krasowska A - Antonie Van Leeuwenhoek (2015)

Bottom Line: When microplates were pre-coated with biosurfactants, PF II was less active than SU, but when cells were incubated together with biosurfactants, the activity of both compounds was similar, independent of the CSH of strains.This suggests irreversible changes in the cell wall after the treatment with biosurfactants.Preincubation of C. albicans with biosurfactants caused extraction of cell wall proteins with molecular mass in the range of 10-40 kDa, which is one possible mechanism of action of the tested lipopeptides.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biotechnology, University of Wrocław, ul. Fryderyka Joliot-Curie 14a, 50-383, Wrocław, Poland.

ABSTRACT
A serious problem for humans is the propensity of Candida albicans to adhere to various surfaces and its ability to form biofilms. Surfactants or biosurfactants can affect the cell surfaces of microorganisms and block their adhesion to different substrates. This study investigated adhesion of C. albicans strains differing in cell surface hydrophobicity (CSH) to polystyrene microplates in order to compare the ability of lipopeptide biosurfactants pseudofactin (PF II) and surfactin (SU) to prevent fungal adhesion to polystyrene. The biosurfactants decreased adhesion of tested strains by 35-90 % when microplates were conditioned before the addition of cells. A 80-90 % reduction of adhesion was observed when cells were incubated together with lipopeptides in microplates. When microplates were pre-coated with biosurfactants, PF II was less active than SU, but when cells were incubated together with biosurfactants, the activity of both compounds was similar, independent of the CSH of strains. When cells were preincubated with lipopeptides and then the compounds were washed out, the adhesion of hydrophobic strains increased two times in comparison to control samples. This suggests irreversible changes in the cell wall after the treatment with biosurfactants. CSH of hydrophobic strains decreased only by 20-60 % after incubation with biosurfactants while adhesion decreased by 80-90 %; the changes in cell adhesion can be thus only partially explained through the modification of CSH. Preincubation of C. albicans with biosurfactants caused extraction of cell wall proteins with molecular mass in the range of 10-40 kDa, which is one possible mechanism of action of the tested lipopeptides.

No MeSH data available.


Related in: MedlinePlus

SDS-PAGE electrophoresis of proteins obtained after incubation of C. albicans cells with 0.1 mg/ml PF II or 0.015 mg/ml SU in comparison to control samples (C)
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Fig7: SDS-PAGE electrophoresis of proteins obtained after incubation of C. albicans cells with 0.1 mg/ml PF II or 0.015 mg/ml SU in comparison to control samples (C)

Mentions: One of the mechanisms of action of lipopeptides on C. albicans cells could be a decrease in the level of some compounds (e.g. chitin, β-1,3-glucan) in the cell wall (Bizerra et al. 2011). Some protocols for the fractionation of fungal cell walls include treatment with synthetic surfactants (Pitarch et al. 2002; Klis et al. 2007). Therefore, we isolated several proteins from cell-free supernatants after preincubation of C. albicans cells with biosurfactants and visualized them on silver-stained polyacrylamide gels (Fig. 7). We determined molecular masses of these proteins after SDS-PAGE electrophoresis to be in the range from ~10 to 40 kDa and observed no differences between the action of PF II and SU or between hydrophobic and hydrophilic strains (Fig. 7). Simultaneously, PAS (Periodic acid-Schiff) staining for glycoproteins showed no bands on the gels (data not shown). Therefore, partial disruption of cell wall and extraction of cell surface-associated proteins can be the possible mechanism of the action of lipopeptide biosurfactants on C. albicans.Fig. 7


The lipopeptides pseudofactin II and surfactin effectively decrease Candida albicans adhesion and hydrophobicity.

Biniarz P, Baranowska G, Feder-Kubis J, Krasowska A - Antonie Van Leeuwenhoek (2015)

SDS-PAGE electrophoresis of proteins obtained after incubation of C. albicans cells with 0.1 mg/ml PF II or 0.015 mg/ml SU in comparison to control samples (C)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4491367&req=5

Fig7: SDS-PAGE electrophoresis of proteins obtained after incubation of C. albicans cells with 0.1 mg/ml PF II or 0.015 mg/ml SU in comparison to control samples (C)
Mentions: One of the mechanisms of action of lipopeptides on C. albicans cells could be a decrease in the level of some compounds (e.g. chitin, β-1,3-glucan) in the cell wall (Bizerra et al. 2011). Some protocols for the fractionation of fungal cell walls include treatment with synthetic surfactants (Pitarch et al. 2002; Klis et al. 2007). Therefore, we isolated several proteins from cell-free supernatants after preincubation of C. albicans cells with biosurfactants and visualized them on silver-stained polyacrylamide gels (Fig. 7). We determined molecular masses of these proteins after SDS-PAGE electrophoresis to be in the range from ~10 to 40 kDa and observed no differences between the action of PF II and SU or between hydrophobic and hydrophilic strains (Fig. 7). Simultaneously, PAS (Periodic acid-Schiff) staining for glycoproteins showed no bands on the gels (data not shown). Therefore, partial disruption of cell wall and extraction of cell surface-associated proteins can be the possible mechanism of the action of lipopeptide biosurfactants on C. albicans.Fig. 7

Bottom Line: When microplates were pre-coated with biosurfactants, PF II was less active than SU, but when cells were incubated together with biosurfactants, the activity of both compounds was similar, independent of the CSH of strains.This suggests irreversible changes in the cell wall after the treatment with biosurfactants.Preincubation of C. albicans with biosurfactants caused extraction of cell wall proteins with molecular mass in the range of 10-40 kDa, which is one possible mechanism of action of the tested lipopeptides.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biotechnology, University of Wrocław, ul. Fryderyka Joliot-Curie 14a, 50-383, Wrocław, Poland.

ABSTRACT
A serious problem for humans is the propensity of Candida albicans to adhere to various surfaces and its ability to form biofilms. Surfactants or biosurfactants can affect the cell surfaces of microorganisms and block their adhesion to different substrates. This study investigated adhesion of C. albicans strains differing in cell surface hydrophobicity (CSH) to polystyrene microplates in order to compare the ability of lipopeptide biosurfactants pseudofactin (PF II) and surfactin (SU) to prevent fungal adhesion to polystyrene. The biosurfactants decreased adhesion of tested strains by 35-90 % when microplates were conditioned before the addition of cells. A 80-90 % reduction of adhesion was observed when cells were incubated together with lipopeptides in microplates. When microplates were pre-coated with biosurfactants, PF II was less active than SU, but when cells were incubated together with biosurfactants, the activity of both compounds was similar, independent of the CSH of strains. When cells were preincubated with lipopeptides and then the compounds were washed out, the adhesion of hydrophobic strains increased two times in comparison to control samples. This suggests irreversible changes in the cell wall after the treatment with biosurfactants. CSH of hydrophobic strains decreased only by 20-60 % after incubation with biosurfactants while adhesion decreased by 80-90 %; the changes in cell adhesion can be thus only partially explained through the modification of CSH. Preincubation of C. albicans with biosurfactants caused extraction of cell wall proteins with molecular mass in the range of 10-40 kDa, which is one possible mechanism of action of the tested lipopeptides.

No MeSH data available.


Related in: MedlinePlus