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The lipopeptides pseudofactin II and surfactin effectively decrease Candida albicans adhesion and hydrophobicity.

Biniarz P, Baranowska G, Feder-Kubis J, Krasowska A - Antonie Van Leeuwenhoek (2015)

Bottom Line: When microplates were pre-coated with biosurfactants, PF II was less active than SU, but when cells were incubated together with biosurfactants, the activity of both compounds was similar, independent of the CSH of strains.This suggests irreversible changes in the cell wall after the treatment with biosurfactants.Preincubation of C. albicans with biosurfactants caused extraction of cell wall proteins with molecular mass in the range of 10-40 kDa, which is one possible mechanism of action of the tested lipopeptides.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biotechnology, University of Wrocław, ul. Fryderyka Joliot-Curie 14a, 50-383, Wrocław, Poland.

ABSTRACT
A serious problem for humans is the propensity of Candida albicans to adhere to various surfaces and its ability to form biofilms. Surfactants or biosurfactants can affect the cell surfaces of microorganisms and block their adhesion to different substrates. This study investigated adhesion of C. albicans strains differing in cell surface hydrophobicity (CSH) to polystyrene microplates in order to compare the ability of lipopeptide biosurfactants pseudofactin (PF II) and surfactin (SU) to prevent fungal adhesion to polystyrene. The biosurfactants decreased adhesion of tested strains by 35-90 % when microplates were conditioned before the addition of cells. A 80-90 % reduction of adhesion was observed when cells were incubated together with lipopeptides in microplates. When microplates were pre-coated with biosurfactants, PF II was less active than SU, but when cells were incubated together with biosurfactants, the activity of both compounds was similar, independent of the CSH of strains. When cells were preincubated with lipopeptides and then the compounds were washed out, the adhesion of hydrophobic strains increased two times in comparison to control samples. This suggests irreversible changes in the cell wall after the treatment with biosurfactants. CSH of hydrophobic strains decreased only by 20-60 % after incubation with biosurfactants while adhesion decreased by 80-90 %; the changes in cell adhesion can be thus only partially explained through the modification of CSH. Preincubation of C. albicans with biosurfactants caused extraction of cell wall proteins with molecular mass in the range of 10-40 kDa, which is one possible mechanism of action of the tested lipopeptides.

No MeSH data available.


Related in: MedlinePlus

Cell surface hydrophobicity (CSH) of C. albicans strains pretreated with 0.035 mg/ml (grey bars) and 0.1 mg/ml (inverse hatched bars) PF II in PBS or 0.005 mg/ml (hatched bars) and 0.015 mg/ml (white bars) SU in PBS, compared to control samples (black bars). The CSH was measured in the presence of biosurfactants in Candida suspension (a) or after rinsing out the surface-active compounds (b). Statistical analysis was performed by modified paired t test *P < 0.05, **P < 0.01, ***P < 0.001
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Fig6: Cell surface hydrophobicity (CSH) of C. albicans strains pretreated with 0.035 mg/ml (grey bars) and 0.1 mg/ml (inverse hatched bars) PF II in PBS or 0.005 mg/ml (hatched bars) and 0.015 mg/ml (white bars) SU in PBS, compared to control samples (black bars). The CSH was measured in the presence of biosurfactants in Candida suspension (a) or after rinsing out the surface-active compounds (b). Statistical analysis was performed by modified paired t test *P < 0.05, **P < 0.01, ***P < 0.001

Mentions: After a 2-h incubation with PF II or SU, CSH of C. albicans CAF4-2 and DSY653 significantly decreased and this effect was concentration-dependent. Monomers of PF II influenced CAF4-2 and DSY653 more strongly than monomers of SU. Other tested strains seemed resistant to the influence of biosurfactants (Fig. 6a). On the other hand, when biosurfactants were washed out, CSH level of hydrophobic cells recovered (Fig. 6b). In this assay the time of incubation with biosurfactants was 2 h and these conditions can be compared to experiments with adhesion of cells treated with biosurfactants (Fig. 4). CSH of hydrophobic strains decreased only by 20–60 % (Fig. 6) while adhesion decreased by 80–90 % (Fig. 4). Also the potential irreversible changes in the cell surface of C. albicans caused by lipopeptides have an impact on adhesion but not on CSH of hydrophobic strains (cf. Figs. 5, 6). This result suggests that decrease in cell adhesion by lipopeptides can be only partially explained by the modification of CSH and should be considered only in the case of hydrophobic strains CAF4-2 and DSY653.Fig. 6


The lipopeptides pseudofactin II and surfactin effectively decrease Candida albicans adhesion and hydrophobicity.

Biniarz P, Baranowska G, Feder-Kubis J, Krasowska A - Antonie Van Leeuwenhoek (2015)

Cell surface hydrophobicity (CSH) of C. albicans strains pretreated with 0.035 mg/ml (grey bars) and 0.1 mg/ml (inverse hatched bars) PF II in PBS or 0.005 mg/ml (hatched bars) and 0.015 mg/ml (white bars) SU in PBS, compared to control samples (black bars). The CSH was measured in the presence of biosurfactants in Candida suspension (a) or after rinsing out the surface-active compounds (b). Statistical analysis was performed by modified paired t test *P < 0.05, **P < 0.01, ***P < 0.001
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4491367&req=5

Fig6: Cell surface hydrophobicity (CSH) of C. albicans strains pretreated with 0.035 mg/ml (grey bars) and 0.1 mg/ml (inverse hatched bars) PF II in PBS or 0.005 mg/ml (hatched bars) and 0.015 mg/ml (white bars) SU in PBS, compared to control samples (black bars). The CSH was measured in the presence of biosurfactants in Candida suspension (a) or after rinsing out the surface-active compounds (b). Statistical analysis was performed by modified paired t test *P < 0.05, **P < 0.01, ***P < 0.001
Mentions: After a 2-h incubation with PF II or SU, CSH of C. albicans CAF4-2 and DSY653 significantly decreased and this effect was concentration-dependent. Monomers of PF II influenced CAF4-2 and DSY653 more strongly than monomers of SU. Other tested strains seemed resistant to the influence of biosurfactants (Fig. 6a). On the other hand, when biosurfactants were washed out, CSH level of hydrophobic cells recovered (Fig. 6b). In this assay the time of incubation with biosurfactants was 2 h and these conditions can be compared to experiments with adhesion of cells treated with biosurfactants (Fig. 4). CSH of hydrophobic strains decreased only by 20–60 % (Fig. 6) while adhesion decreased by 80–90 % (Fig. 4). Also the potential irreversible changes in the cell surface of C. albicans caused by lipopeptides have an impact on adhesion but not on CSH of hydrophobic strains (cf. Figs. 5, 6). This result suggests that decrease in cell adhesion by lipopeptides can be only partially explained by the modification of CSH and should be considered only in the case of hydrophobic strains CAF4-2 and DSY653.Fig. 6

Bottom Line: When microplates were pre-coated with biosurfactants, PF II was less active than SU, but when cells were incubated together with biosurfactants, the activity of both compounds was similar, independent of the CSH of strains.This suggests irreversible changes in the cell wall after the treatment with biosurfactants.Preincubation of C. albicans with biosurfactants caused extraction of cell wall proteins with molecular mass in the range of 10-40 kDa, which is one possible mechanism of action of the tested lipopeptides.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biotechnology, University of Wrocław, ul. Fryderyka Joliot-Curie 14a, 50-383, Wrocław, Poland.

ABSTRACT
A serious problem for humans is the propensity of Candida albicans to adhere to various surfaces and its ability to form biofilms. Surfactants or biosurfactants can affect the cell surfaces of microorganisms and block their adhesion to different substrates. This study investigated adhesion of C. albicans strains differing in cell surface hydrophobicity (CSH) to polystyrene microplates in order to compare the ability of lipopeptide biosurfactants pseudofactin (PF II) and surfactin (SU) to prevent fungal adhesion to polystyrene. The biosurfactants decreased adhesion of tested strains by 35-90 % when microplates were conditioned before the addition of cells. A 80-90 % reduction of adhesion was observed when cells were incubated together with lipopeptides in microplates. When microplates were pre-coated with biosurfactants, PF II was less active than SU, but when cells were incubated together with biosurfactants, the activity of both compounds was similar, independent of the CSH of strains. When cells were preincubated with lipopeptides and then the compounds were washed out, the adhesion of hydrophobic strains increased two times in comparison to control samples. This suggests irreversible changes in the cell wall after the treatment with biosurfactants. CSH of hydrophobic strains decreased only by 20-60 % after incubation with biosurfactants while adhesion decreased by 80-90 %; the changes in cell adhesion can be thus only partially explained through the modification of CSH. Preincubation of C. albicans with biosurfactants caused extraction of cell wall proteins with molecular mass in the range of 10-40 kDa, which is one possible mechanism of action of the tested lipopeptides.

No MeSH data available.


Related in: MedlinePlus