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The lipopeptides pseudofactin II and surfactin effectively decrease Candida albicans adhesion and hydrophobicity.

Biniarz P, Baranowska G, Feder-Kubis J, Krasowska A - Antonie Van Leeuwenhoek (2015)

Bottom Line: When microplates were pre-coated with biosurfactants, PF II was less active than SU, but when cells were incubated together with biosurfactants, the activity of both compounds was similar, independent of the CSH of strains.This suggests irreversible changes in the cell wall after the treatment with biosurfactants.Preincubation of C. albicans with biosurfactants caused extraction of cell wall proteins with molecular mass in the range of 10-40 kDa, which is one possible mechanism of action of the tested lipopeptides.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biotechnology, University of Wrocław, ul. Fryderyka Joliot-Curie 14a, 50-383, Wrocław, Poland.

ABSTRACT
A serious problem for humans is the propensity of Candida albicans to adhere to various surfaces and its ability to form biofilms. Surfactants or biosurfactants can affect the cell surfaces of microorganisms and block their adhesion to different substrates. This study investigated adhesion of C. albicans strains differing in cell surface hydrophobicity (CSH) to polystyrene microplates in order to compare the ability of lipopeptide biosurfactants pseudofactin (PF II) and surfactin (SU) to prevent fungal adhesion to polystyrene. The biosurfactants decreased adhesion of tested strains by 35-90 % when microplates were conditioned before the addition of cells. A 80-90 % reduction of adhesion was observed when cells were incubated together with lipopeptides in microplates. When microplates were pre-coated with biosurfactants, PF II was less active than SU, but when cells were incubated together with biosurfactants, the activity of both compounds was similar, independent of the CSH of strains. When cells were preincubated with lipopeptides and then the compounds were washed out, the adhesion of hydrophobic strains increased two times in comparison to control samples. This suggests irreversible changes in the cell wall after the treatment with biosurfactants. CSH of hydrophobic strains decreased only by 20-60 % after incubation with biosurfactants while adhesion decreased by 80-90 %; the changes in cell adhesion can be thus only partially explained through the modification of CSH. Preincubation of C. albicans with biosurfactants caused extraction of cell wall proteins with molecular mass in the range of 10-40 kDa, which is one possible mechanism of action of the tested lipopeptides.

No MeSH data available.


Related in: MedlinePlus

Adhesion of C. albicans strains to polystyrene microplates pretreated with 0.035 mg/ml (grey bars) and 0.1 mg/ml (inverse-hatched bars) PF II in PBS or 0.005 mg/ml (hatched bars) and 0.015 mg/ml (white bars) SU in PBS, compared to control adhesion (black bars). Statistical analysis was performed with modified paired t test *P < 0.05, **P < 0.01, ***P < 0.001
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Fig3: Adhesion of C. albicans strains to polystyrene microplates pretreated with 0.035 mg/ml (grey bars) and 0.1 mg/ml (inverse-hatched bars) PF II in PBS or 0.005 mg/ml (hatched bars) and 0.015 mg/ml (white bars) SU in PBS, compared to control adhesion (black bars). Statistical analysis was performed with modified paired t test *P < 0.05, **P < 0.01, ***P < 0.001

Mentions: We observed a decrease in adhesion of all tested C. albicans strains when the microplates were pretreated with PF II before the addition of the microorganisms (pre-adhesion assay) (Fig. 3). PF II was more active in concentrations higher than CMC (0.1 mg/ml) (Fig. 3). We observed a similar concentration-dependent effect for SU used as a standard lipopeptide biosurfactant, which decreased the adhesion even more than PF II (P < 0.001) (Fig. 3). CAF4-2 and DSY653 adhered to the polystyrene microplate surface better than the other strains (P < 0.01) and were able to adhere to a surface pretreated with lipopeptides more strongly than other strains (P < 0.001) (Fig. 3).Fig. 3


The lipopeptides pseudofactin II and surfactin effectively decrease Candida albicans adhesion and hydrophobicity.

Biniarz P, Baranowska G, Feder-Kubis J, Krasowska A - Antonie Van Leeuwenhoek (2015)

Adhesion of C. albicans strains to polystyrene microplates pretreated with 0.035 mg/ml (grey bars) and 0.1 mg/ml (inverse-hatched bars) PF II in PBS or 0.005 mg/ml (hatched bars) and 0.015 mg/ml (white bars) SU in PBS, compared to control adhesion (black bars). Statistical analysis was performed with modified paired t test *P < 0.05, **P < 0.01, ***P < 0.001
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4491367&req=5

Fig3: Adhesion of C. albicans strains to polystyrene microplates pretreated with 0.035 mg/ml (grey bars) and 0.1 mg/ml (inverse-hatched bars) PF II in PBS or 0.005 mg/ml (hatched bars) and 0.015 mg/ml (white bars) SU in PBS, compared to control adhesion (black bars). Statistical analysis was performed with modified paired t test *P < 0.05, **P < 0.01, ***P < 0.001
Mentions: We observed a decrease in adhesion of all tested C. albicans strains when the microplates were pretreated with PF II before the addition of the microorganisms (pre-adhesion assay) (Fig. 3). PF II was more active in concentrations higher than CMC (0.1 mg/ml) (Fig. 3). We observed a similar concentration-dependent effect for SU used as a standard lipopeptide biosurfactant, which decreased the adhesion even more than PF II (P < 0.001) (Fig. 3). CAF4-2 and DSY653 adhered to the polystyrene microplate surface better than the other strains (P < 0.01) and were able to adhere to a surface pretreated with lipopeptides more strongly than other strains (P < 0.001) (Fig. 3).Fig. 3

Bottom Line: When microplates were pre-coated with biosurfactants, PF II was less active than SU, but when cells were incubated together with biosurfactants, the activity of both compounds was similar, independent of the CSH of strains.This suggests irreversible changes in the cell wall after the treatment with biosurfactants.Preincubation of C. albicans with biosurfactants caused extraction of cell wall proteins with molecular mass in the range of 10-40 kDa, which is one possible mechanism of action of the tested lipopeptides.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biotechnology, University of Wrocław, ul. Fryderyka Joliot-Curie 14a, 50-383, Wrocław, Poland.

ABSTRACT
A serious problem for humans is the propensity of Candida albicans to adhere to various surfaces and its ability to form biofilms. Surfactants or biosurfactants can affect the cell surfaces of microorganisms and block their adhesion to different substrates. This study investigated adhesion of C. albicans strains differing in cell surface hydrophobicity (CSH) to polystyrene microplates in order to compare the ability of lipopeptide biosurfactants pseudofactin (PF II) and surfactin (SU) to prevent fungal adhesion to polystyrene. The biosurfactants decreased adhesion of tested strains by 35-90 % when microplates were conditioned before the addition of cells. A 80-90 % reduction of adhesion was observed when cells were incubated together with lipopeptides in microplates. When microplates were pre-coated with biosurfactants, PF II was less active than SU, but when cells were incubated together with biosurfactants, the activity of both compounds was similar, independent of the CSH of strains. When cells were preincubated with lipopeptides and then the compounds were washed out, the adhesion of hydrophobic strains increased two times in comparison to control samples. This suggests irreversible changes in the cell wall after the treatment with biosurfactants. CSH of hydrophobic strains decreased only by 20-60 % after incubation with biosurfactants while adhesion decreased by 80-90 %; the changes in cell adhesion can be thus only partially explained through the modification of CSH. Preincubation of C. albicans with biosurfactants caused extraction of cell wall proteins with molecular mass in the range of 10-40 kDa, which is one possible mechanism of action of the tested lipopeptides.

No MeSH data available.


Related in: MedlinePlus