Simplification of the modified Gallyas method.
Bottom Line: However, this method is not popularly used in general pathology laboratories because of the need for special reagents, several staining processes, and skilled techniques.We omitted the lanthanum nitrate step from the staining process and verified the adequacy in comparison with the original method as well as immunohistochemistry, using specimens from patients of Alzheimer's disease, argyrophilic grain disease, multiple system atrophy, Pick's disease, and Lewy body disease.The lanthanum nitrate step can be omitted from the modified Gallyas method, resulting in reduction in the number of reagents required and shortening of the staining time.
Affiliation: Department of Pathology and Laboratory Medicine, National Center Hospital of Neurology and Psychiatry.Show MeSH
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Mentions: About fixation of the specimens, PFA fixation caused a non-specific background reaction involving red blood cells and blood vessels that was not observed with formalin fixation. However, this background staining did not cause a problem in diagnosis because the abnormal structures in each disease were absent of background staining (Fig. 3). Both the modified Gallyas and the simplified Gallyas method showed good sensitivity without non-specific staining of blood vessels or red blood cells regardless of the fixation period.
Affiliation: Department of Pathology and Laboratory Medicine, National Center Hospital of Neurology and Psychiatry.