Limits...
Simplification of the modified Gallyas method.

Kuninaka N, Kawaguchi M, Ogawa M, Sato A, Arima K, Murayama S, Saito Y - Neuropathology (2014)

Bottom Line: In Japan, the Gallyas method has improved and is widely used as the "modified Gallyas method".The simplified method provided good staining to all the structures in archival tissues, compared with the modified Gallyas method in a significantly shorter staining time.The lanthanum nitrate step can be omitted from the modified Gallyas method, resulting in reduction in the number of reagents required and shortening of the staining time.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology and Laboratory Medicine, National Center Hospital of Neurology and Psychiatry.

Show MeSH

Related in: MedlinePlus

Comparison between formalin and PFA fixation solutions (representative case: dementia with grains). (A): Formalin fixation. Argyrophilic granules were observed in numbers (black arrows). Non-specific reactions were not observed in peripheral blood vessels, red blood cells, etc. (B): PFA fixation. Although non-specific reactions were observed in peripheral blood vessels and red blood cells, these non-specific reactions did not influence the silver reaction itself (white arrows).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4491351&req=5

fig03: Comparison between formalin and PFA fixation solutions (representative case: dementia with grains). (A): Formalin fixation. Argyrophilic granules were observed in numbers (black arrows). Non-specific reactions were not observed in peripheral blood vessels, red blood cells, etc. (B): PFA fixation. Although non-specific reactions were observed in peripheral blood vessels and red blood cells, these non-specific reactions did not influence the silver reaction itself (white arrows).

Mentions: About fixation of the specimens, PFA fixation caused a non-specific background reaction involving red blood cells and blood vessels that was not observed with formalin fixation. However, this background staining did not cause a problem in diagnosis because the abnormal structures in each disease were absent of background staining (Fig. 3). Both the modified Gallyas and the simplified Gallyas method showed good sensitivity without non-specific staining of blood vessels or red blood cells regardless of the fixation period.


Simplification of the modified Gallyas method.

Kuninaka N, Kawaguchi M, Ogawa M, Sato A, Arima K, Murayama S, Saito Y - Neuropathology (2014)

Comparison between formalin and PFA fixation solutions (representative case: dementia with grains). (A): Formalin fixation. Argyrophilic granules were observed in numbers (black arrows). Non-specific reactions were not observed in peripheral blood vessels, red blood cells, etc. (B): PFA fixation. Although non-specific reactions were observed in peripheral blood vessels and red blood cells, these non-specific reactions did not influence the silver reaction itself (white arrows).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4491351&req=5

fig03: Comparison between formalin and PFA fixation solutions (representative case: dementia with grains). (A): Formalin fixation. Argyrophilic granules were observed in numbers (black arrows). Non-specific reactions were not observed in peripheral blood vessels, red blood cells, etc. (B): PFA fixation. Although non-specific reactions were observed in peripheral blood vessels and red blood cells, these non-specific reactions did not influence the silver reaction itself (white arrows).
Mentions: About fixation of the specimens, PFA fixation caused a non-specific background reaction involving red blood cells and blood vessels that was not observed with formalin fixation. However, this background staining did not cause a problem in diagnosis because the abnormal structures in each disease were absent of background staining (Fig. 3). Both the modified Gallyas and the simplified Gallyas method showed good sensitivity without non-specific staining of blood vessels or red blood cells regardless of the fixation period.

Bottom Line: In Japan, the Gallyas method has improved and is widely used as the "modified Gallyas method".The simplified method provided good staining to all the structures in archival tissues, compared with the modified Gallyas method in a significantly shorter staining time.The lanthanum nitrate step can be omitted from the modified Gallyas method, resulting in reduction in the number of reagents required and shortening of the staining time.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology and Laboratory Medicine, National Center Hospital of Neurology and Psychiatry.

Show MeSH
Related in: MedlinePlus