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PPL2ab neurons restore sexual responses in aged Drosophila males through dopamine.

Kuo SY, Wu CL, Hsieh MY, Lin CT, Wen RK, Chen LC, Chen YH, Yu YW, Wang HD, Su YJ, Lin CJ, Yang CY, Guan HY, Wang PY, Lan TH, Fu TF - Nat Commun (2015)

Bottom Line: We find that increasing DA levels in a subset of cells in the PPL2ab neuronal cluster is necessary and sufficient for increased sustained courtship in both young and aged male flies.Our results indicate that preventing the age-related decline in DA levels in PPL2ab neurons alleviates diminished courtship behaviours in male Drosophila.These results may provide the foundation for deciphering the circuitry involved in sexual motivation in the male Drosophila brain.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Chemistry, National Chi Nan University, 54561 Nantou, Taiwan.

ABSTRACT
Male sexual desire typically declines with ageing. However, our understanding of the neurobiological basis for this phenomenon is limited by our knowledge of the brain circuitry and neuronal pathways controlling male sexual desire. A number of studies across species suggest that dopamine (DA) affects sexual desire. Here we use genetic tools and behavioural assays to identify a novel subset of DA neurons that regulate age-associated male courtship activity in Drosophila. We find that increasing DA levels in a subset of cells in the PPL2ab neuronal cluster is necessary and sufficient for increased sustained courtship in both young and aged male flies. Our results indicate that preventing the age-related decline in DA levels in PPL2ab neurons alleviates diminished courtship behaviours in male Drosophila. These results may provide the foundation for deciphering the circuitry involved in sexual motivation in the male Drosophila brain.

No MeSH data available.


PPL2ab neurons affect male courtship sustainment through DA.(a) A diagram of the LexPR/LexAop (or GeneSwitch/UAS)-inducible system, which was used to temporally increase DA levels specifically in Gal4- and LexA-expressing neurons. Males aged 35 days were fed 1.5 mM RU486 (+) for 5 days, and the courtship strength was tested at the 40-day-old time point. There were significant differences in the courtship index (b) and courtship bout length (c) in the flies that received RU486 and carried the murashka-1-Gal4>UAS-LexPR; LexAop-TH or LG121-LexA>LexAop-GeneSwitch;UAS-TH compared with flies of the same genotype that did not receive RU486 treatment, as well as for the corresponding heterozygous driver and effector genotypes. Each column represents the mean of 15 tests. (d) Decreased DA levels brought about in the LexPR/LexAop-thRNAi (or GeneSwitch/UAS-thRNAi)-inducible system in the PPL2ab neurons inhibited mature male courtship sustainment. Males aged 5 days were fed 1.5 mM RU486 (+) for 5 days and tested for courtship strength at 10 days old. We did not observe any significant differences in the courtship index (e) and courtship bout length (f) in the flies that carried murashka-1-Gal4; UAS-Dcr2>UAS-LexPR;LexAop-thRNAi or LG121-LexA;UAS-Dcr2>LexAop-GeneSwitch;UAS-thRNAi as compared with flies of the same genotype that did not receive RU486 treatment, as well as for the corresponding heterozygous driver and effector genotypes. Each column represents the mean of 15 tests. The error bars indicate+s.e.m. The means for columns followed by the same letters were not significantly different at the threshold of P<0.05 by two-way ANOVA followed by a Bonferroni multiple-comparisons test per grouped columns (separated by a dashed line).
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f4: PPL2ab neurons affect male courtship sustainment through DA.(a) A diagram of the LexPR/LexAop (or GeneSwitch/UAS)-inducible system, which was used to temporally increase DA levels specifically in Gal4- and LexA-expressing neurons. Males aged 35 days were fed 1.5 mM RU486 (+) for 5 days, and the courtship strength was tested at the 40-day-old time point. There were significant differences in the courtship index (b) and courtship bout length (c) in the flies that received RU486 and carried the murashka-1-Gal4>UAS-LexPR; LexAop-TH or LG121-LexA>LexAop-GeneSwitch;UAS-TH compared with flies of the same genotype that did not receive RU486 treatment, as well as for the corresponding heterozygous driver and effector genotypes. Each column represents the mean of 15 tests. (d) Decreased DA levels brought about in the LexPR/LexAop-thRNAi (or GeneSwitch/UAS-thRNAi)-inducible system in the PPL2ab neurons inhibited mature male courtship sustainment. Males aged 5 days were fed 1.5 mM RU486 (+) for 5 days and tested for courtship strength at 10 days old. We did not observe any significant differences in the courtship index (e) and courtship bout length (f) in the flies that carried murashka-1-Gal4; UAS-Dcr2>UAS-LexPR;LexAop-thRNAi or LG121-LexA;UAS-Dcr2>LexAop-GeneSwitch;UAS-thRNAi as compared with flies of the same genotype that did not receive RU486 treatment, as well as for the corresponding heterozygous driver and effector genotypes. Each column represents the mean of 15 tests. The error bars indicate+s.e.m. The means for columns followed by the same letters were not significantly different at the threshold of P<0.05 by two-way ANOVA followed by a Bonferroni multiple-comparisons test per grouped columns (separated by a dashed line).

Mentions: We wondered whether the diminished courtship sustainment in aged male flies might be restored by increasing the DA levels in PPL2ab neurons. We temporally induced DA expression in PPL2ab neurons from day 35 to 40 post-eclosion and tested courtship sustainment in the 40-day-old flies (Fig. 4a), we found that the courtship index and courtship bout length were significantly increased in aged males (two-way ANOVA followed by a Bonferroni multiple-comparisons test, P<0.05) (Fig. 4b,c). In contrast, when we induced downregulated DA levels in PPL2ab neurons from day 5 to 10 post-eclosion and tested courtship sustainment in 10-day-old flies (Fig. 4d), we observed a significant decrease in both the courtship index and courtship bout length in mature males (two-way ANOVA followed by a Bonferroni multiple-comparisons test, P<0.05) (Fig. 4e,f). There was a non-significant trend for decreasing courtship bout length towards the decapitated target female in flies with downregulated th in LG121 neurons versus the control group (two-way ANOVA followed by a Bonferroni multiple-comparisons test, P>0.05).


PPL2ab neurons restore sexual responses in aged Drosophila males through dopamine.

Kuo SY, Wu CL, Hsieh MY, Lin CT, Wen RK, Chen LC, Chen YH, Yu YW, Wang HD, Su YJ, Lin CJ, Yang CY, Guan HY, Wang PY, Lan TH, Fu TF - Nat Commun (2015)

PPL2ab neurons affect male courtship sustainment through DA.(a) A diagram of the LexPR/LexAop (or GeneSwitch/UAS)-inducible system, which was used to temporally increase DA levels specifically in Gal4- and LexA-expressing neurons. Males aged 35 days were fed 1.5 mM RU486 (+) for 5 days, and the courtship strength was tested at the 40-day-old time point. There were significant differences in the courtship index (b) and courtship bout length (c) in the flies that received RU486 and carried the murashka-1-Gal4>UAS-LexPR; LexAop-TH or LG121-LexA>LexAop-GeneSwitch;UAS-TH compared with flies of the same genotype that did not receive RU486 treatment, as well as for the corresponding heterozygous driver and effector genotypes. Each column represents the mean of 15 tests. (d) Decreased DA levels brought about in the LexPR/LexAop-thRNAi (or GeneSwitch/UAS-thRNAi)-inducible system in the PPL2ab neurons inhibited mature male courtship sustainment. Males aged 5 days were fed 1.5 mM RU486 (+) for 5 days and tested for courtship strength at 10 days old. We did not observe any significant differences in the courtship index (e) and courtship bout length (f) in the flies that carried murashka-1-Gal4; UAS-Dcr2>UAS-LexPR;LexAop-thRNAi or LG121-LexA;UAS-Dcr2>LexAop-GeneSwitch;UAS-thRNAi as compared with flies of the same genotype that did not receive RU486 treatment, as well as for the corresponding heterozygous driver and effector genotypes. Each column represents the mean of 15 tests. The error bars indicate+s.e.m. The means for columns followed by the same letters were not significantly different at the threshold of P<0.05 by two-way ANOVA followed by a Bonferroni multiple-comparisons test per grouped columns (separated by a dashed line).
© Copyright Policy - open-access
Related In: Results  -  Collection

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f4: PPL2ab neurons affect male courtship sustainment through DA.(a) A diagram of the LexPR/LexAop (or GeneSwitch/UAS)-inducible system, which was used to temporally increase DA levels specifically in Gal4- and LexA-expressing neurons. Males aged 35 days were fed 1.5 mM RU486 (+) for 5 days, and the courtship strength was tested at the 40-day-old time point. There were significant differences in the courtship index (b) and courtship bout length (c) in the flies that received RU486 and carried the murashka-1-Gal4>UAS-LexPR; LexAop-TH or LG121-LexA>LexAop-GeneSwitch;UAS-TH compared with flies of the same genotype that did not receive RU486 treatment, as well as for the corresponding heterozygous driver and effector genotypes. Each column represents the mean of 15 tests. (d) Decreased DA levels brought about in the LexPR/LexAop-thRNAi (or GeneSwitch/UAS-thRNAi)-inducible system in the PPL2ab neurons inhibited mature male courtship sustainment. Males aged 5 days were fed 1.5 mM RU486 (+) for 5 days and tested for courtship strength at 10 days old. We did not observe any significant differences in the courtship index (e) and courtship bout length (f) in the flies that carried murashka-1-Gal4; UAS-Dcr2>UAS-LexPR;LexAop-thRNAi or LG121-LexA;UAS-Dcr2>LexAop-GeneSwitch;UAS-thRNAi as compared with flies of the same genotype that did not receive RU486 treatment, as well as for the corresponding heterozygous driver and effector genotypes. Each column represents the mean of 15 tests. The error bars indicate+s.e.m. The means for columns followed by the same letters were not significantly different at the threshold of P<0.05 by two-way ANOVA followed by a Bonferroni multiple-comparisons test per grouped columns (separated by a dashed line).
Mentions: We wondered whether the diminished courtship sustainment in aged male flies might be restored by increasing the DA levels in PPL2ab neurons. We temporally induced DA expression in PPL2ab neurons from day 35 to 40 post-eclosion and tested courtship sustainment in the 40-day-old flies (Fig. 4a), we found that the courtship index and courtship bout length were significantly increased in aged males (two-way ANOVA followed by a Bonferroni multiple-comparisons test, P<0.05) (Fig. 4b,c). In contrast, when we induced downregulated DA levels in PPL2ab neurons from day 5 to 10 post-eclosion and tested courtship sustainment in 10-day-old flies (Fig. 4d), we observed a significant decrease in both the courtship index and courtship bout length in mature males (two-way ANOVA followed by a Bonferroni multiple-comparisons test, P<0.05) (Fig. 4e,f). There was a non-significant trend for decreasing courtship bout length towards the decapitated target female in flies with downregulated th in LG121 neurons versus the control group (two-way ANOVA followed by a Bonferroni multiple-comparisons test, P>0.05).

Bottom Line: We find that increasing DA levels in a subset of cells in the PPL2ab neuronal cluster is necessary and sufficient for increased sustained courtship in both young and aged male flies.Our results indicate that preventing the age-related decline in DA levels in PPL2ab neurons alleviates diminished courtship behaviours in male Drosophila.These results may provide the foundation for deciphering the circuitry involved in sexual motivation in the male Drosophila brain.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Chemistry, National Chi Nan University, 54561 Nantou, Taiwan.

ABSTRACT
Male sexual desire typically declines with ageing. However, our understanding of the neurobiological basis for this phenomenon is limited by our knowledge of the brain circuitry and neuronal pathways controlling male sexual desire. A number of studies across species suggest that dopamine (DA) affects sexual desire. Here we use genetic tools and behavioural assays to identify a novel subset of DA neurons that regulate age-associated male courtship activity in Drosophila. We find that increasing DA levels in a subset of cells in the PPL2ab neuronal cluster is necessary and sufficient for increased sustained courtship in both young and aged male flies. Our results indicate that preventing the age-related decline in DA levels in PPL2ab neurons alleviates diminished courtship behaviours in male Drosophila. These results may provide the foundation for deciphering the circuitry involved in sexual motivation in the male Drosophila brain.

No MeSH data available.