Limits...
PPL2ab neurons restore sexual responses in aged Drosophila males through dopamine.

Kuo SY, Wu CL, Hsieh MY, Lin CT, Wen RK, Chen LC, Chen YH, Yu YW, Wang HD, Su YJ, Lin CJ, Yang CY, Guan HY, Wang PY, Lan TH, Fu TF - Nat Commun (2015)

Bottom Line: We find that increasing DA levels in a subset of cells in the PPL2ab neuronal cluster is necessary and sufficient for increased sustained courtship in both young and aged male flies.Our results indicate that preventing the age-related decline in DA levels in PPL2ab neurons alleviates diminished courtship behaviours in male Drosophila.These results may provide the foundation for deciphering the circuitry involved in sexual motivation in the male Drosophila brain.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Chemistry, National Chi Nan University, 54561 Nantou, Taiwan.

ABSTRACT
Male sexual desire typically declines with ageing. However, our understanding of the neurobiological basis for this phenomenon is limited by our knowledge of the brain circuitry and neuronal pathways controlling male sexual desire. A number of studies across species suggest that dopamine (DA) affects sexual desire. Here we use genetic tools and behavioural assays to identify a novel subset of DA neurons that regulate age-associated male courtship activity in Drosophila. We find that increasing DA levels in a subset of cells in the PPL2ab neuronal cluster is necessary and sufficient for increased sustained courtship in both young and aged male flies. Our results indicate that preventing the age-related decline in DA levels in PPL2ab neurons alleviates diminished courtship behaviours in male Drosophila. These results may provide the foundation for deciphering the circuitry involved in sexual motivation in the male Drosophila brain.

No MeSH data available.


A small subset of DAergic neurons regulates male courtship activity.TH expression was driven by Gal4 drivers with restricted expression in specific DA neurons and the male courtship index was obtained in flies from each line (see below for details). The results are presented in the bar plot. ANOVAs were used to compare genetically manipulated 10-day-old male flies across the different genetic manipulations or with the relevant heterozygous controls. Significant differences were observed in the courtship index in TH-Gal4>UAS-TH, TH-C1-Gal4>UAS-TH, and murashka-1-Gal4>UAS-TH flies compared with the corresponding Gal4 driver and UAS-TH heterozygous flies. Each column represents the mean of 22 tests. The error bars indicate+s.e.m. The means within each column followed by the same letters are not significantly different at the threshold of P<0.05 by a one-way ANOVA followed by Tukey's test. See Supplementary Figs 1 and 2, and Supplementary Table 1 for representative images and a summary of the driver expression patterns. Genotypes: (1) +/y;+/+;+/UAS-TH; (2) +/y;+/+;TH-Gal4/UAS-TH; (3) +/y;+/+;TH-Gal4/+; (4) +/y; TH-C1-Gal4/+; +/UAS-TH; (5) +/y; TH-C1-Gal4/+; +/+; (6) +/y; TH-D1-Gal4/+; +/UAS-TH; (7) +/y; TH-D1-Gal4/+; +/+; (8) +/y;+/+;murashka-1-Gal4/UAS-TH; (9) +/y;+/+;murashka-1-Gal4/+; (10) NP2758-Gal4/y;+/+;+/UAS-TH; (11) NP2758-Gal4/y;+/+;+/+; (12) c061-Gal4/y;MB-Gal80/+;+/UAS-TH; (13) c061-Gal4/y;MB-Gal80/+;+/+; (14) +/y;MB-Gal80/+;krasavietz-Gal4/UAS-TH; (15) +/y;MB-Gal80/+;krasavietz-Gal4/+; (16) +/y;NP1528-Gal4/+;+/UAS-TH; (17) +/y;NP1528-Gal4/+;+/+; (18) +/y;NP5272-Gal4/+;+/UAS-TH; (19) +/y;NP5272-Gal4/+;+/+; (20) +/y;+/+;HL9-62-1N-Gal4/UAS-TH; and (21) +/y;+/+;HL9-62-1N-Gal4/+.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4491191&req=5

f1: A small subset of DAergic neurons regulates male courtship activity.TH expression was driven by Gal4 drivers with restricted expression in specific DA neurons and the male courtship index was obtained in flies from each line (see below for details). The results are presented in the bar plot. ANOVAs were used to compare genetically manipulated 10-day-old male flies across the different genetic manipulations or with the relevant heterozygous controls. Significant differences were observed in the courtship index in TH-Gal4>UAS-TH, TH-C1-Gal4>UAS-TH, and murashka-1-Gal4>UAS-TH flies compared with the corresponding Gal4 driver and UAS-TH heterozygous flies. Each column represents the mean of 22 tests. The error bars indicate+s.e.m. The means within each column followed by the same letters are not significantly different at the threshold of P<0.05 by a one-way ANOVA followed by Tukey's test. See Supplementary Figs 1 and 2, and Supplementary Table 1 for representative images and a summary of the driver expression patterns. Genotypes: (1) +/y;+/+;+/UAS-TH; (2) +/y;+/+;TH-Gal4/UAS-TH; (3) +/y;+/+;TH-Gal4/+; (4) +/y; TH-C1-Gal4/+; +/UAS-TH; (5) +/y; TH-C1-Gal4/+; +/+; (6) +/y; TH-D1-Gal4/+; +/UAS-TH; (7) +/y; TH-D1-Gal4/+; +/+; (8) +/y;+/+;murashka-1-Gal4/UAS-TH; (9) +/y;+/+;murashka-1-Gal4/+; (10) NP2758-Gal4/y;+/+;+/UAS-TH; (11) NP2758-Gal4/y;+/+;+/+; (12) c061-Gal4/y;MB-Gal80/+;+/UAS-TH; (13) c061-Gal4/y;MB-Gal80/+;+/+; (14) +/y;MB-Gal80/+;krasavietz-Gal4/UAS-TH; (15) +/y;MB-Gal80/+;krasavietz-Gal4/+; (16) +/y;NP1528-Gal4/+;+/UAS-TH; (17) +/y;NP1528-Gal4/+;+/+; (18) +/y;NP5272-Gal4/+;+/UAS-TH; (19) +/y;NP5272-Gal4/+;+/+; (20) +/y;+/+;HL9-62-1N-Gal4/UAS-TH; and (21) +/y;+/+;HL9-62-1N-Gal4/+.

Mentions: To determine the specific DA neurons that regulate Drosophila courtship sustainment, we used Gal4 drivers to manipulate DA activity in restricted groups of DAergic neurons in the fly brain (Supplementary Fig. 1; murashka-1-Gal4 driver in Fig. 2a; and summarized in Supplementary Table 1). We then analysed the effect of cell-type-specific DA overexpression on courtship intensity in male flies (Fig. 1). Our results revealed that increased expression of TH in TH-Gal4-(ref. 17) and TH-C1-Gal4-positive18 neurons significantly enhanced the male courtship index (Fig. 1). Interestingly, we observed similar results by overexpressing TH in murashka-1-Gal4-positive neurons, which targets a more restricted cell population (Fig. 1). To label the cells targeted by TH-Gal4, TH-C1-Gal4 and murashka-1-Gal4, we crossed each line to the UAS-mCD8::GFP reporter line and counterstained each by TH immunohistochemistry. By assessing the anatomical profile of TH cells coexpressing GFP in each line, we found that expression overlapped between the three Gal4 drivers in a subset of DA neurons in the PPL2ab (Fig. 2a1 and Supplementary Fig. 1g1) and the protocerebral posteriomedial dopaminergic cluster neuron 1/2 (PPM1/2) (Fig. 2a2 and Supplementary Fig. 1g3). These data suggested that male courtship intensity might be regulated by DA release from these neurons.


PPL2ab neurons restore sexual responses in aged Drosophila males through dopamine.

Kuo SY, Wu CL, Hsieh MY, Lin CT, Wen RK, Chen LC, Chen YH, Yu YW, Wang HD, Su YJ, Lin CJ, Yang CY, Guan HY, Wang PY, Lan TH, Fu TF - Nat Commun (2015)

A small subset of DAergic neurons regulates male courtship activity.TH expression was driven by Gal4 drivers with restricted expression in specific DA neurons and the male courtship index was obtained in flies from each line (see below for details). The results are presented in the bar plot. ANOVAs were used to compare genetically manipulated 10-day-old male flies across the different genetic manipulations or with the relevant heterozygous controls. Significant differences were observed in the courtship index in TH-Gal4>UAS-TH, TH-C1-Gal4>UAS-TH, and murashka-1-Gal4>UAS-TH flies compared with the corresponding Gal4 driver and UAS-TH heterozygous flies. Each column represents the mean of 22 tests. The error bars indicate+s.e.m. The means within each column followed by the same letters are not significantly different at the threshold of P<0.05 by a one-way ANOVA followed by Tukey's test. See Supplementary Figs 1 and 2, and Supplementary Table 1 for representative images and a summary of the driver expression patterns. Genotypes: (1) +/y;+/+;+/UAS-TH; (2) +/y;+/+;TH-Gal4/UAS-TH; (3) +/y;+/+;TH-Gal4/+; (4) +/y; TH-C1-Gal4/+; +/UAS-TH; (5) +/y; TH-C1-Gal4/+; +/+; (6) +/y; TH-D1-Gal4/+; +/UAS-TH; (7) +/y; TH-D1-Gal4/+; +/+; (8) +/y;+/+;murashka-1-Gal4/UAS-TH; (9) +/y;+/+;murashka-1-Gal4/+; (10) NP2758-Gal4/y;+/+;+/UAS-TH; (11) NP2758-Gal4/y;+/+;+/+; (12) c061-Gal4/y;MB-Gal80/+;+/UAS-TH; (13) c061-Gal4/y;MB-Gal80/+;+/+; (14) +/y;MB-Gal80/+;krasavietz-Gal4/UAS-TH; (15) +/y;MB-Gal80/+;krasavietz-Gal4/+; (16) +/y;NP1528-Gal4/+;+/UAS-TH; (17) +/y;NP1528-Gal4/+;+/+; (18) +/y;NP5272-Gal4/+;+/UAS-TH; (19) +/y;NP5272-Gal4/+;+/+; (20) +/y;+/+;HL9-62-1N-Gal4/UAS-TH; and (21) +/y;+/+;HL9-62-1N-Gal4/+.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4491191&req=5

f1: A small subset of DAergic neurons regulates male courtship activity.TH expression was driven by Gal4 drivers with restricted expression in specific DA neurons and the male courtship index was obtained in flies from each line (see below for details). The results are presented in the bar plot. ANOVAs were used to compare genetically manipulated 10-day-old male flies across the different genetic manipulations or with the relevant heterozygous controls. Significant differences were observed in the courtship index in TH-Gal4>UAS-TH, TH-C1-Gal4>UAS-TH, and murashka-1-Gal4>UAS-TH flies compared with the corresponding Gal4 driver and UAS-TH heterozygous flies. Each column represents the mean of 22 tests. The error bars indicate+s.e.m. The means within each column followed by the same letters are not significantly different at the threshold of P<0.05 by a one-way ANOVA followed by Tukey's test. See Supplementary Figs 1 and 2, and Supplementary Table 1 for representative images and a summary of the driver expression patterns. Genotypes: (1) +/y;+/+;+/UAS-TH; (2) +/y;+/+;TH-Gal4/UAS-TH; (3) +/y;+/+;TH-Gal4/+; (4) +/y; TH-C1-Gal4/+; +/UAS-TH; (5) +/y; TH-C1-Gal4/+; +/+; (6) +/y; TH-D1-Gal4/+; +/UAS-TH; (7) +/y; TH-D1-Gal4/+; +/+; (8) +/y;+/+;murashka-1-Gal4/UAS-TH; (9) +/y;+/+;murashka-1-Gal4/+; (10) NP2758-Gal4/y;+/+;+/UAS-TH; (11) NP2758-Gal4/y;+/+;+/+; (12) c061-Gal4/y;MB-Gal80/+;+/UAS-TH; (13) c061-Gal4/y;MB-Gal80/+;+/+; (14) +/y;MB-Gal80/+;krasavietz-Gal4/UAS-TH; (15) +/y;MB-Gal80/+;krasavietz-Gal4/+; (16) +/y;NP1528-Gal4/+;+/UAS-TH; (17) +/y;NP1528-Gal4/+;+/+; (18) +/y;NP5272-Gal4/+;+/UAS-TH; (19) +/y;NP5272-Gal4/+;+/+; (20) +/y;+/+;HL9-62-1N-Gal4/UAS-TH; and (21) +/y;+/+;HL9-62-1N-Gal4/+.
Mentions: To determine the specific DA neurons that regulate Drosophila courtship sustainment, we used Gal4 drivers to manipulate DA activity in restricted groups of DAergic neurons in the fly brain (Supplementary Fig. 1; murashka-1-Gal4 driver in Fig. 2a; and summarized in Supplementary Table 1). We then analysed the effect of cell-type-specific DA overexpression on courtship intensity in male flies (Fig. 1). Our results revealed that increased expression of TH in TH-Gal4-(ref. 17) and TH-C1-Gal4-positive18 neurons significantly enhanced the male courtship index (Fig. 1). Interestingly, we observed similar results by overexpressing TH in murashka-1-Gal4-positive neurons, which targets a more restricted cell population (Fig. 1). To label the cells targeted by TH-Gal4, TH-C1-Gal4 and murashka-1-Gal4, we crossed each line to the UAS-mCD8::GFP reporter line and counterstained each by TH immunohistochemistry. By assessing the anatomical profile of TH cells coexpressing GFP in each line, we found that expression overlapped between the three Gal4 drivers in a subset of DA neurons in the PPL2ab (Fig. 2a1 and Supplementary Fig. 1g1) and the protocerebral posteriomedial dopaminergic cluster neuron 1/2 (PPM1/2) (Fig. 2a2 and Supplementary Fig. 1g3). These data suggested that male courtship intensity might be regulated by DA release from these neurons.

Bottom Line: We find that increasing DA levels in a subset of cells in the PPL2ab neuronal cluster is necessary and sufficient for increased sustained courtship in both young and aged male flies.Our results indicate that preventing the age-related decline in DA levels in PPL2ab neurons alleviates diminished courtship behaviours in male Drosophila.These results may provide the foundation for deciphering the circuitry involved in sexual motivation in the male Drosophila brain.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Chemistry, National Chi Nan University, 54561 Nantou, Taiwan.

ABSTRACT
Male sexual desire typically declines with ageing. However, our understanding of the neurobiological basis for this phenomenon is limited by our knowledge of the brain circuitry and neuronal pathways controlling male sexual desire. A number of studies across species suggest that dopamine (DA) affects sexual desire. Here we use genetic tools and behavioural assays to identify a novel subset of DA neurons that regulate age-associated male courtship activity in Drosophila. We find that increasing DA levels in a subset of cells in the PPL2ab neuronal cluster is necessary and sufficient for increased sustained courtship in both young and aged male flies. Our results indicate that preventing the age-related decline in DA levels in PPL2ab neurons alleviates diminished courtship behaviours in male Drosophila. These results may provide the foundation for deciphering the circuitry involved in sexual motivation in the male Drosophila brain.

No MeSH data available.