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Two-dimensional isobutyl acetate production pathways to improve carbon yield.

Tashiro Y, Desai SH, Atsumi S - Nat Commun (2015)

Bottom Line: To avoid these problems, we describe here the construction of a metabolic pathway that simultaneously utilizes glucose and acetate.We demonstrate the utility of this approach for isobutyl acetate (IBA) production, wherein IBA production with glucose and acetate achieves a higher carbon yield than with either sole carbon source.These results highlight the potential for this multiple carbon source approach to improve the TMCY and balance redox in biosynthetic pathways.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, University of California, Davis, One Shields Avenue, Davis, California 95616, USA.

ABSTRACT
For an economically competitive biological process, achieving high carbon yield of a target chemical is crucial. In biochemical production, pyruvate and acetyl-CoA are primary building blocks. When sugar is used as the sole biosynthetic substrate, acetyl-CoA is commonly generated by pyruvate decarboxylation. However, pyruvate decarboxylation during acetyl-CoA formation limits the theoretical maximum carbon yield (TMCY) by releasing carbon, and in some cases also leads to redox imbalance. To avoid these problems, we describe here the construction of a metabolic pathway that simultaneously utilizes glucose and acetate. Acetate is utilized to produce acetyl-CoA without carbon loss or redox imbalance. We demonstrate the utility of this approach for isobutyl acetate (IBA) production, wherein IBA production with glucose and acetate achieves a higher carbon yield than with either sole carbon source. These results highlight the potential for this multiple carbon source approach to improve the TMCY and balance redox in biosynthetic pathways.

No MeSH data available.


Related in: MedlinePlus

Isotope tracing of the IBA synthesis pathway using 13C glucose and 13C acetate.(a) IBA structure and predicted MS fragmentation pattern. The location of predicted 13C is represented by the number 13 adjacent to the carbon molecules. IBA was produced using unlabelled substrate (b,e), 12C glucose and 13C acetate (c,f) or 13C glucose and 12C acetate (d,g) in strain 7 (b–d) or 8 (e–g).
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f4: Isotope tracing of the IBA synthesis pathway using 13C glucose and 13C acetate.(a) IBA structure and predicted MS fragmentation pattern. The location of predicted 13C is represented by the number 13 adjacent to the carbon molecules. IBA was produced using unlabelled substrate (b,e), 12C glucose and 13C acetate (c,f) or 13C glucose and 12C acetate (d,g) in strain 7 (b–d) or 8 (e–g).

Mentions: To investigate that both glucose and acetate are contributing towards IBA production, isotopic tracing analysis using 13C glucose or 13C acetate was performed. Strains 7 and 8 (Table 1) were grown with several combinations of carbon sources for IBA production and analysed by capillary gas chromatography mass spectrometry (GC–MS; (Fig. 4).


Two-dimensional isobutyl acetate production pathways to improve carbon yield.

Tashiro Y, Desai SH, Atsumi S - Nat Commun (2015)

Isotope tracing of the IBA synthesis pathway using 13C glucose and 13C acetate.(a) IBA structure and predicted MS fragmentation pattern. The location of predicted 13C is represented by the number 13 adjacent to the carbon molecules. IBA was produced using unlabelled substrate (b,e), 12C glucose and 13C acetate (c,f) or 13C glucose and 12C acetate (d,g) in strain 7 (b–d) or 8 (e–g).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4491173&req=5

f4: Isotope tracing of the IBA synthesis pathway using 13C glucose and 13C acetate.(a) IBA structure and predicted MS fragmentation pattern. The location of predicted 13C is represented by the number 13 adjacent to the carbon molecules. IBA was produced using unlabelled substrate (b,e), 12C glucose and 13C acetate (c,f) or 13C glucose and 12C acetate (d,g) in strain 7 (b–d) or 8 (e–g).
Mentions: To investigate that both glucose and acetate are contributing towards IBA production, isotopic tracing analysis using 13C glucose or 13C acetate was performed. Strains 7 and 8 (Table 1) were grown with several combinations of carbon sources for IBA production and analysed by capillary gas chromatography mass spectrometry (GC–MS; (Fig. 4).

Bottom Line: To avoid these problems, we describe here the construction of a metabolic pathway that simultaneously utilizes glucose and acetate.We demonstrate the utility of this approach for isobutyl acetate (IBA) production, wherein IBA production with glucose and acetate achieves a higher carbon yield than with either sole carbon source.These results highlight the potential for this multiple carbon source approach to improve the TMCY and balance redox in biosynthetic pathways.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, University of California, Davis, One Shields Avenue, Davis, California 95616, USA.

ABSTRACT
For an economically competitive biological process, achieving high carbon yield of a target chemical is crucial. In biochemical production, pyruvate and acetyl-CoA are primary building blocks. When sugar is used as the sole biosynthetic substrate, acetyl-CoA is commonly generated by pyruvate decarboxylation. However, pyruvate decarboxylation during acetyl-CoA formation limits the theoretical maximum carbon yield (TMCY) by releasing carbon, and in some cases also leads to redox imbalance. To avoid these problems, we describe here the construction of a metabolic pathway that simultaneously utilizes glucose and acetate. Acetate is utilized to produce acetyl-CoA without carbon loss or redox imbalance. We demonstrate the utility of this approach for isobutyl acetate (IBA) production, wherein IBA production with glucose and acetate achieves a higher carbon yield than with either sole carbon source. These results highlight the potential for this multiple carbon source approach to improve the TMCY and balance redox in biosynthetic pathways.

No MeSH data available.


Related in: MedlinePlus