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In Situ Regeneration of Si-based ARROW-B Surface Plasmon Resonance Biosensors.

Hsu HF, Lin YT, Huang YT, Lu MF, Chen CH - J Med Biol Eng (2015)

Bottom Line: SPR was used to monitor the regeneration processes.The experimental results show that the sensing response did not significantly change after the sensor was reused more than 10 times.In situ regenerations of the sensors were achieved.

View Article: PubMed Central - PubMed

Affiliation: Department of Electronic Engineering and Institute of Electronics, National Chiao Tung University, Hsinchu, Taiwan.

ABSTRACT

Si-based antiresonant reflecting optical waveguide type B (ARROW-B) surface plasmon resonance (SPR) biosensors allow label-free high-sensitivity detection of biomolecular interactions in real time. The ARROW-B waveguide, which has a thick guiding layer, provides efficient coupling with a single-mode fiber. The Si-based ARROW-B SPR biosensors were fabricated by using the standard semiconductor fabrication processes with a single-step lithography. A fluid flow system was designed to transport samples or analytes. The waveguide consists of propagation and SPR sensing regions. The propagation regions in the front and rear of the SPR sensing region have a symmetric cladding structure to isolate them from environmental changes. A high-index O-ring is used to seal the liquid flow channel. The intensity interrogation method was used to characterize the sensors. The sensitivity of the biosensors was 3.0 × 10(3) µW/RIU (refractive index unit) with a resolution of 6.2 × 10(-5) RIU. An in situ regeneration process was designed to make the sensors reusable and eliminate re-alignment of the optical measurement system. The regeneration was realized using ammonia-hydrogen peroxide mixture solutions to remove molecules bound on the sensor surface, such as self-assembled 11-mercapto-1undecanoic acid and bovine serum albumin. SPR was used to monitor the regeneration processes. The experimental results show that the sensing response did not significantly change after the sensor was reused more than 10 times. In situ regenerations of the sensors were achieved.

No MeSH data available.


a–d SPR responses of four real-time regenerations
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Fig9: a–d SPR responses of four real-time regenerations

Mentions: The results of repeated experiments of the removal of 11-MUA from the gold surface are shown in Fig. 8. The SPR responses of the PBS baseline and 11-MUA were 26.45 ± 1.02 and 14.70 ± 3.06 μW, respectively. Though the SPR responses of 11-MUA showed some variation, which might have resulted from inaccurate experiment control of 11-MUA binding, the variation of SPR responses of the PBS baseline was less than 4 % after 12 regenerations, confirming the reusability of this sensor. Figure 9 shows real-time measurement results of four regenerations with 11-MUA and BSA binding. In each regeneration, the SPR responses in the PBS baselines after 11-MUA and BSA bindings are clearly changed. The numerical analysis of Fig. 9 is shown in Fig. 10. The SPR response of BSA is 9.71 ± 1.52 μW. The results show the capability of biomolecular immobilization on the sensor. Therefore, the reusability of this sensor was verified. This reusable ARROW-B SPR biosensor can also detect other biomolecules being able to be bounded with the 11-MUA, e.g., peptides and proteins.Fig. 9


In Situ Regeneration of Si-based ARROW-B Surface Plasmon Resonance Biosensors.

Hsu HF, Lin YT, Huang YT, Lu MF, Chen CH - J Med Biol Eng (2015)

a–d SPR responses of four real-time regenerations
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4491115&req=5

Fig9: a–d SPR responses of four real-time regenerations
Mentions: The results of repeated experiments of the removal of 11-MUA from the gold surface are shown in Fig. 8. The SPR responses of the PBS baseline and 11-MUA were 26.45 ± 1.02 and 14.70 ± 3.06 μW, respectively. Though the SPR responses of 11-MUA showed some variation, which might have resulted from inaccurate experiment control of 11-MUA binding, the variation of SPR responses of the PBS baseline was less than 4 % after 12 regenerations, confirming the reusability of this sensor. Figure 9 shows real-time measurement results of four regenerations with 11-MUA and BSA binding. In each regeneration, the SPR responses in the PBS baselines after 11-MUA and BSA bindings are clearly changed. The numerical analysis of Fig. 9 is shown in Fig. 10. The SPR response of BSA is 9.71 ± 1.52 μW. The results show the capability of biomolecular immobilization on the sensor. Therefore, the reusability of this sensor was verified. This reusable ARROW-B SPR biosensor can also detect other biomolecules being able to be bounded with the 11-MUA, e.g., peptides and proteins.Fig. 9

Bottom Line: SPR was used to monitor the regeneration processes.The experimental results show that the sensing response did not significantly change after the sensor was reused more than 10 times.In situ regenerations of the sensors were achieved.

View Article: PubMed Central - PubMed

Affiliation: Department of Electronic Engineering and Institute of Electronics, National Chiao Tung University, Hsinchu, Taiwan.

ABSTRACT

Si-based antiresonant reflecting optical waveguide type B (ARROW-B) surface plasmon resonance (SPR) biosensors allow label-free high-sensitivity detection of biomolecular interactions in real time. The ARROW-B waveguide, which has a thick guiding layer, provides efficient coupling with a single-mode fiber. The Si-based ARROW-B SPR biosensors were fabricated by using the standard semiconductor fabrication processes with a single-step lithography. A fluid flow system was designed to transport samples or analytes. The waveguide consists of propagation and SPR sensing regions. The propagation regions in the front and rear of the SPR sensing region have a symmetric cladding structure to isolate them from environmental changes. A high-index O-ring is used to seal the liquid flow channel. The intensity interrogation method was used to characterize the sensors. The sensitivity of the biosensors was 3.0 × 10(3) µW/RIU (refractive index unit) with a resolution of 6.2 × 10(-5) RIU. An in situ regeneration process was designed to make the sensors reusable and eliminate re-alignment of the optical measurement system. The regeneration was realized using ammonia-hydrogen peroxide mixture solutions to remove molecules bound on the sensor surface, such as self-assembled 11-mercapto-1undecanoic acid and bovine serum albumin. SPR was used to monitor the regeneration processes. The experimental results show that the sensing response did not significantly change after the sensor was reused more than 10 times. In situ regenerations of the sensors were achieved.

No MeSH data available.