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In Situ Regeneration of Si-based ARROW-B Surface Plasmon Resonance Biosensors.

Hsu HF, Lin YT, Huang YT, Lu MF, Chen CH - J Med Biol Eng (2015)

Bottom Line: SPR was used to monitor the regeneration processes.The experimental results show that the sensing response did not significantly change after the sensor was reused more than 10 times.In situ regenerations of the sensors were achieved.

View Article: PubMed Central - PubMed

Affiliation: Department of Electronic Engineering and Institute of Electronics, National Chiao Tung University, Hsinchu, Taiwan.

ABSTRACT

Si-based antiresonant reflecting optical waveguide type B (ARROW-B) surface plasmon resonance (SPR) biosensors allow label-free high-sensitivity detection of biomolecular interactions in real time. The ARROW-B waveguide, which has a thick guiding layer, provides efficient coupling with a single-mode fiber. The Si-based ARROW-B SPR biosensors were fabricated by using the standard semiconductor fabrication processes with a single-step lithography. A fluid flow system was designed to transport samples or analytes. The waveguide consists of propagation and SPR sensing regions. The propagation regions in the front and rear of the SPR sensing region have a symmetric cladding structure to isolate them from environmental changes. A high-index O-ring is used to seal the liquid flow channel. The intensity interrogation method was used to characterize the sensors. The sensitivity of the biosensors was 3.0 × 10(3) µW/RIU (refractive index unit) with a resolution of 6.2 × 10(-5) RIU. An in situ regeneration process was designed to make the sensors reusable and eliminate re-alignment of the optical measurement system. The regeneration was realized using ammonia-hydrogen peroxide mixture solutions to remove molecules bound on the sensor surface, such as self-assembled 11-mercapto-1undecanoic acid and bovine serum albumin. SPR was used to monitor the regeneration processes. The experimental results show that the sensing response did not significantly change after the sensor was reused more than 10 times. In situ regenerations of the sensors were achieved.

No MeSH data available.


a Real-time measurement results of water, and 0.5, 1 and 2 NaCl solutions. b Comparison of experimental and simulation results
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Fig7: a Real-time measurement results of water, and 0.5, 1 and 2 NaCl solutions. b Comparison of experimental and simulation results

Mentions: NaCl solutions at various concentrations were flowed onto the sensor surface to measure the output power variations for demonstrating the feasibility of the proposed sensor chips. Because the refractive index varies with sodium chloride concentrations, the output power varied with sodium chloride concentrations. The refractive index of d.d. water is 1.3330, and those of 0.5, 1, and 2 M sodium chloride solutions are 1.3381, 1.3430 and 1.3523, respectively. The refractive indices of all sample solutions were measured using a digital refractometer with the precision of ±0.0001. The higher the refractive index of sodium chloride solution, the higher was the detected output power. This tendency agrees with the simulation results. The real-time measurement results and a comparison with the transfer matrix method simulation results are shown in Fig. 7. The sensitivity of the proposed sensor is defined as the ratio of the output power variation to the change of the environment refractive index. The experimental results indicate that the output power was approximately a linear function of the refractive index. The sensitivity of this chip was about 3.0 × 103 μW/RIU. The standard deviation of the output signal of the d.d. water was 0.185 μW and the corresponding resolution was 6.2 × 10−5 RIU [18].Fig. 8


In Situ Regeneration of Si-based ARROW-B Surface Plasmon Resonance Biosensors.

Hsu HF, Lin YT, Huang YT, Lu MF, Chen CH - J Med Biol Eng (2015)

a Real-time measurement results of water, and 0.5, 1 and 2 NaCl solutions. b Comparison of experimental and simulation results
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4491115&req=5

Fig7: a Real-time measurement results of water, and 0.5, 1 and 2 NaCl solutions. b Comparison of experimental and simulation results
Mentions: NaCl solutions at various concentrations were flowed onto the sensor surface to measure the output power variations for demonstrating the feasibility of the proposed sensor chips. Because the refractive index varies with sodium chloride concentrations, the output power varied with sodium chloride concentrations. The refractive index of d.d. water is 1.3330, and those of 0.5, 1, and 2 M sodium chloride solutions are 1.3381, 1.3430 and 1.3523, respectively. The refractive indices of all sample solutions were measured using a digital refractometer with the precision of ±0.0001. The higher the refractive index of sodium chloride solution, the higher was the detected output power. This tendency agrees with the simulation results. The real-time measurement results and a comparison with the transfer matrix method simulation results are shown in Fig. 7. The sensitivity of the proposed sensor is defined as the ratio of the output power variation to the change of the environment refractive index. The experimental results indicate that the output power was approximately a linear function of the refractive index. The sensitivity of this chip was about 3.0 × 103 μW/RIU. The standard deviation of the output signal of the d.d. water was 0.185 μW and the corresponding resolution was 6.2 × 10−5 RIU [18].Fig. 8

Bottom Line: SPR was used to monitor the regeneration processes.The experimental results show that the sensing response did not significantly change after the sensor was reused more than 10 times.In situ regenerations of the sensors were achieved.

View Article: PubMed Central - PubMed

Affiliation: Department of Electronic Engineering and Institute of Electronics, National Chiao Tung University, Hsinchu, Taiwan.

ABSTRACT

Si-based antiresonant reflecting optical waveguide type B (ARROW-B) surface plasmon resonance (SPR) biosensors allow label-free high-sensitivity detection of biomolecular interactions in real time. The ARROW-B waveguide, which has a thick guiding layer, provides efficient coupling with a single-mode fiber. The Si-based ARROW-B SPR biosensors were fabricated by using the standard semiconductor fabrication processes with a single-step lithography. A fluid flow system was designed to transport samples or analytes. The waveguide consists of propagation and SPR sensing regions. The propagation regions in the front and rear of the SPR sensing region have a symmetric cladding structure to isolate them from environmental changes. A high-index O-ring is used to seal the liquid flow channel. The intensity interrogation method was used to characterize the sensors. The sensitivity of the biosensors was 3.0 × 10(3) µW/RIU (refractive index unit) with a resolution of 6.2 × 10(-5) RIU. An in situ regeneration process was designed to make the sensors reusable and eliminate re-alignment of the optical measurement system. The regeneration was realized using ammonia-hydrogen peroxide mixture solutions to remove molecules bound on the sensor surface, such as self-assembled 11-mercapto-1undecanoic acid and bovine serum albumin. SPR was used to monitor the regeneration processes. The experimental results show that the sensing response did not significantly change after the sensor was reused more than 10 times. In situ regenerations of the sensors were achieved.

No MeSH data available.