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In Situ Regeneration of Si-based ARROW-B Surface Plasmon Resonance Biosensors.

Hsu HF, Lin YT, Huang YT, Lu MF, Chen CH - J Med Biol Eng (2015)

Bottom Line: SPR was used to monitor the regeneration processes.The experimental results show that the sensing response did not significantly change after the sensor was reused more than 10 times.In situ regenerations of the sensors were achieved.

View Article: PubMed Central - PubMed

Affiliation: Department of Electronic Engineering and Institute of Electronics, National Chiao Tung University, Hsinchu, Taiwan.

ABSTRACT

Si-based antiresonant reflecting optical waveguide type B (ARROW-B) surface plasmon resonance (SPR) biosensors allow label-free high-sensitivity detection of biomolecular interactions in real time. The ARROW-B waveguide, which has a thick guiding layer, provides efficient coupling with a single-mode fiber. The Si-based ARROW-B SPR biosensors were fabricated by using the standard semiconductor fabrication processes with a single-step lithography. A fluid flow system was designed to transport samples or analytes. The waveguide consists of propagation and SPR sensing regions. The propagation regions in the front and rear of the SPR sensing region have a symmetric cladding structure to isolate them from environmental changes. A high-index O-ring is used to seal the liquid flow channel. The intensity interrogation method was used to characterize the sensors. The sensitivity of the biosensors was 3.0 × 10(3) µW/RIU (refractive index unit) with a resolution of 6.2 × 10(-5) RIU. An in situ regeneration process was designed to make the sensors reusable and eliminate re-alignment of the optical measurement system. The regeneration was realized using ammonia-hydrogen peroxide mixture solutions to remove molecules bound on the sensor surface, such as self-assembled 11-mercapto-1undecanoic acid and bovine serum albumin. SPR was used to monitor the regeneration processes. The experimental results show that the sensing response did not significantly change after the sensor was reused more than 10 times. In situ regenerations of the sensors were achieved.

No MeSH data available.


Flow chart of regeneration process
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Fig6: Flow chart of regeneration process

Mentions: An optical measurement system was set up to perform the bioassay experiments, as shown in Fig. 6. In this optical measurement system, a He–Ne laser with the wavelength 0.6328 μm was used as the light source to excite SPWs in the Au sensing region. Since SPWs can be excited only by TM-polarized waves, the laser beam first passed through a polarizer, and only vertically polarized electric field remained. Then, the vertically polarized light was focused into the input channel of the waveguide by an objective lens (10×). At the output end, the light was collected by an objective lens (20×). Finally, the intensity of the output power was collected by a photodiode. In addition, a fixing stage for the SPR sensor chip was utilized. The freshly cleaned sensor chip was mounted onto this stage so that the input light was able to be focused into one of the waveguides. To construct a seamless liquid flow channel on the sensor chip, an O-ring was added on the chip and sandwiched by two plastic slides. For the fluid flow system, a peristaltic pump was used to propel liquid samples in a plastic tube with a 1.6-mm inner diameter to flow through the sensing region of the chip. The flow system was controlled to have a flow rate of 300 μl/min.Fig. 6


In Situ Regeneration of Si-based ARROW-B Surface Plasmon Resonance Biosensors.

Hsu HF, Lin YT, Huang YT, Lu MF, Chen CH - J Med Biol Eng (2015)

Flow chart of regeneration process
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4491115&req=5

Fig6: Flow chart of regeneration process
Mentions: An optical measurement system was set up to perform the bioassay experiments, as shown in Fig. 6. In this optical measurement system, a He–Ne laser with the wavelength 0.6328 μm was used as the light source to excite SPWs in the Au sensing region. Since SPWs can be excited only by TM-polarized waves, the laser beam first passed through a polarizer, and only vertically polarized electric field remained. Then, the vertically polarized light was focused into the input channel of the waveguide by an objective lens (10×). At the output end, the light was collected by an objective lens (20×). Finally, the intensity of the output power was collected by a photodiode. In addition, a fixing stage for the SPR sensor chip was utilized. The freshly cleaned sensor chip was mounted onto this stage so that the input light was able to be focused into one of the waveguides. To construct a seamless liquid flow channel on the sensor chip, an O-ring was added on the chip and sandwiched by two plastic slides. For the fluid flow system, a peristaltic pump was used to propel liquid samples in a plastic tube with a 1.6-mm inner diameter to flow through the sensing region of the chip. The flow system was controlled to have a flow rate of 300 μl/min.Fig. 6

Bottom Line: SPR was used to monitor the regeneration processes.The experimental results show that the sensing response did not significantly change after the sensor was reused more than 10 times.In situ regenerations of the sensors were achieved.

View Article: PubMed Central - PubMed

Affiliation: Department of Electronic Engineering and Institute of Electronics, National Chiao Tung University, Hsinchu, Taiwan.

ABSTRACT

Si-based antiresonant reflecting optical waveguide type B (ARROW-B) surface plasmon resonance (SPR) biosensors allow label-free high-sensitivity detection of biomolecular interactions in real time. The ARROW-B waveguide, which has a thick guiding layer, provides efficient coupling with a single-mode fiber. The Si-based ARROW-B SPR biosensors were fabricated by using the standard semiconductor fabrication processes with a single-step lithography. A fluid flow system was designed to transport samples or analytes. The waveguide consists of propagation and SPR sensing regions. The propagation regions in the front and rear of the SPR sensing region have a symmetric cladding structure to isolate them from environmental changes. A high-index O-ring is used to seal the liquid flow channel. The intensity interrogation method was used to characterize the sensors. The sensitivity of the biosensors was 3.0 × 10(3) µW/RIU (refractive index unit) with a resolution of 6.2 × 10(-5) RIU. An in situ regeneration process was designed to make the sensors reusable and eliminate re-alignment of the optical measurement system. The regeneration was realized using ammonia-hydrogen peroxide mixture solutions to remove molecules bound on the sensor surface, such as self-assembled 11-mercapto-1undecanoic acid and bovine serum albumin. SPR was used to monitor the regeneration processes. The experimental results show that the sensing response did not significantly change after the sensor was reused more than 10 times. In situ regenerations of the sensors were achieved.

No MeSH data available.