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In Situ Regeneration of Si-based ARROW-B Surface Plasmon Resonance Biosensors.

Hsu HF, Lin YT, Huang YT, Lu MF, Chen CH - J Med Biol Eng (2015)

Bottom Line: SPR was used to monitor the regeneration processes.The experimental results show that the sensing response did not significantly change after the sensor was reused more than 10 times.In situ regenerations of the sensors were achieved.

View Article: PubMed Central - PubMed

Affiliation: Department of Electronic Engineering and Institute of Electronics, National Chiao Tung University, Hsinchu, Taiwan.

ABSTRACT

Si-based antiresonant reflecting optical waveguide type B (ARROW-B) surface plasmon resonance (SPR) biosensors allow label-free high-sensitivity detection of biomolecular interactions in real time. The ARROW-B waveguide, which has a thick guiding layer, provides efficient coupling with a single-mode fiber. The Si-based ARROW-B SPR biosensors were fabricated by using the standard semiconductor fabrication processes with a single-step lithography. A fluid flow system was designed to transport samples or analytes. The waveguide consists of propagation and SPR sensing regions. The propagation regions in the front and rear of the SPR sensing region have a symmetric cladding structure to isolate them from environmental changes. A high-index O-ring is used to seal the liquid flow channel. The intensity interrogation method was used to characterize the sensors. The sensitivity of the biosensors was 3.0 × 10(3) µW/RIU (refractive index unit) with a resolution of 6.2 × 10(-5) RIU. An in situ regeneration process was designed to make the sensors reusable and eliminate re-alignment of the optical measurement system. The regeneration was realized using ammonia-hydrogen peroxide mixture solutions to remove molecules bound on the sensor surface, such as self-assembled 11-mercapto-1undecanoic acid and bovine serum albumin. SPR was used to monitor the regeneration processes. The experimental results show that the sensing response did not significantly change after the sensor was reused more than 10 times. In situ regenerations of the sensors were achieved.

No MeSH data available.


Flow chart of fabrication process for ARROW-B SPR sensor chip
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Fig3: Flow chart of fabrication process for ARROW-B SPR sensor chip

Mentions: The length of the sensing region should be considered to moderately excite SPWs on the Au surface. If the length of the sensing region is too long, the light will be largely consumed in the Au sensing region, making detection difficult. If the length of the sensing region is too short, the guided mode in the ARROW waveguide could not effectively interact with the SPW at the interface of the metal, and thus the response becomes small. As shown in Fig. 3, the long sensing length resulted in relatively large SPR loss without a shift of the SPR dip. The sensitivity was improved, but measurement equipment with a high resolution was required. Thus, the length of sensing region Ls was selected as 4 mm to obtain an ARROW-B SPR sensor chip with sufficiently high sensitivity. Also, this sensing length was long enough for transferring the TM-polarized wave to the gold film surface and for exciting SPWs. The lengths of the front and rear regions were also chosen as 4 mm, which were long enough for the wave propagation to be stable before and after the light passed through the sensing region. One advantage of adopting ARROW-B SPR devices is compatibility with current semiconductor processing technology, allowing mass production.Fig. 3


In Situ Regeneration of Si-based ARROW-B Surface Plasmon Resonance Biosensors.

Hsu HF, Lin YT, Huang YT, Lu MF, Chen CH - J Med Biol Eng (2015)

Flow chart of fabrication process for ARROW-B SPR sensor chip
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4491115&req=5

Fig3: Flow chart of fabrication process for ARROW-B SPR sensor chip
Mentions: The length of the sensing region should be considered to moderately excite SPWs on the Au surface. If the length of the sensing region is too long, the light will be largely consumed in the Au sensing region, making detection difficult. If the length of the sensing region is too short, the guided mode in the ARROW waveguide could not effectively interact with the SPW at the interface of the metal, and thus the response becomes small. As shown in Fig. 3, the long sensing length resulted in relatively large SPR loss without a shift of the SPR dip. The sensitivity was improved, but measurement equipment with a high resolution was required. Thus, the length of sensing region Ls was selected as 4 mm to obtain an ARROW-B SPR sensor chip with sufficiently high sensitivity. Also, this sensing length was long enough for transferring the TM-polarized wave to the gold film surface and for exciting SPWs. The lengths of the front and rear regions were also chosen as 4 mm, which were long enough for the wave propagation to be stable before and after the light passed through the sensing region. One advantage of adopting ARROW-B SPR devices is compatibility with current semiconductor processing technology, allowing mass production.Fig. 3

Bottom Line: SPR was used to monitor the regeneration processes.The experimental results show that the sensing response did not significantly change after the sensor was reused more than 10 times.In situ regenerations of the sensors were achieved.

View Article: PubMed Central - PubMed

Affiliation: Department of Electronic Engineering and Institute of Electronics, National Chiao Tung University, Hsinchu, Taiwan.

ABSTRACT

Si-based antiresonant reflecting optical waveguide type B (ARROW-B) surface plasmon resonance (SPR) biosensors allow label-free high-sensitivity detection of biomolecular interactions in real time. The ARROW-B waveguide, which has a thick guiding layer, provides efficient coupling with a single-mode fiber. The Si-based ARROW-B SPR biosensors were fabricated by using the standard semiconductor fabrication processes with a single-step lithography. A fluid flow system was designed to transport samples or analytes. The waveguide consists of propagation and SPR sensing regions. The propagation regions in the front and rear of the SPR sensing region have a symmetric cladding structure to isolate them from environmental changes. A high-index O-ring is used to seal the liquid flow channel. The intensity interrogation method was used to characterize the sensors. The sensitivity of the biosensors was 3.0 × 10(3) µW/RIU (refractive index unit) with a resolution of 6.2 × 10(-5) RIU. An in situ regeneration process was designed to make the sensors reusable and eliminate re-alignment of the optical measurement system. The regeneration was realized using ammonia-hydrogen peroxide mixture solutions to remove molecules bound on the sensor surface, such as self-assembled 11-mercapto-1undecanoic acid and bovine serum albumin. SPR was used to monitor the regeneration processes. The experimental results show that the sensing response did not significantly change after the sensor was reused more than 10 times. In situ regenerations of the sensors were achieved.

No MeSH data available.