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Modulation of Silica Nanoparticle Uptake into Human Osteoblast Cells by Variation of the Ratio of Amino and Sulfonate Surface Groups: Effects of Serum.

Shahabi S, Treccani L, Dringen R, Rezwan K - ACS Appl Mater Interfaces (2015)

Bottom Line: Irrespective of the original surface charge, serum proteins adsorbed onto the surface, neutralized the zeta potential values, and prevented the aggregation of the tailor-made FFSNPs.In contrast, in serum-containing medium, anionic FFSNPs were internalized by HOB cells more strongly, despite the similar size and surface charge of all types of protein-covered FFSNPs.Thus, at physiological condition, when the presence of proteins is inevitable, sulfonate-functionalized silica NPs are the favorite choice to achieve a desired high rate of NP internalization.

View Article: PubMed Central - PubMed

Affiliation: †Advanced Ceramics, University of Bremen, Am Biologischen Garten 2, 28359 Bremen, Germany.

ABSTRACT
To study the importance of the surface charge for cellular uptake of silica nanoparticles (NPs), we synthesized five different single- or multifunctionalized fluorescent silica NPs (FFSNPs) by introducing various ratios of amino and sulfonate groups into their surface. The zeta potential values of these FFSNPs were customized from highly positive to highly negative, while other physicochemical properties remained almost constant. Irrespective of the original surface charge, serum proteins adsorbed onto the surface, neutralized the zeta potential values, and prevented the aggregation of the tailor-made FFSNPs. Depending on the surface charge and on the absence or presence of serum, two opposite trends were found concerning the cellular uptake of FFSNPs. In the absence of serum, positively charged NPs were more strongly accumulated by human osteoblast (HOB) cells than negatively charged NPs. In contrast, in serum-containing medium, anionic FFSNPs were internalized by HOB cells more strongly, despite the similar size and surface charge of all types of protein-covered FFSNPs. Thus, at physiological condition, when the presence of proteins is inevitable, sulfonate-functionalized silica NPs are the favorite choice to achieve a desired high rate of NP internalization.

No MeSH data available.


Related in: MedlinePlus

SEM and STEM (insets)micrographs of FFSNPs (a) and fluorescence emission spectra of FFSNPsaqueous suspensions at 550 nm excitation and fluorescence microscopymicrographs (b). Scale bars are 200 nm, 10 nm, and 20 μm inall SEM, STEM, and fluorescence microscopy images, respectively.
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fig1: SEM and STEM (insets)micrographs of FFSNPs (a) and fluorescence emission spectra of FFSNPsaqueous suspensions at 550 nm excitation and fluorescence microscopymicrographs (b). Scale bars are 200 nm, 10 nm, and 20 μm inall SEM, STEM, and fluorescence microscopy images, respectively.

Mentions: SEM and STEM analysis revealed that all types of FFSNPs synthesizedare of spherical shape and show a narrow size distribution (Figure 1a) with a mean diameter of 58 nm (Table 1). The fluorescent emission spectra of the FFSNPswere measured at the excitation wavelength of RBITC (550 nm) and areshown in Figure 1b. All FFSNPs presented awell distinct fluorescence signal at the emission wavelength of RBITC.This was further confirmed by fluorescence microscopy, which indicatedthat all FFSNPs feature bright fluorescence.


Modulation of Silica Nanoparticle Uptake into Human Osteoblast Cells by Variation of the Ratio of Amino and Sulfonate Surface Groups: Effects of Serum.

Shahabi S, Treccani L, Dringen R, Rezwan K - ACS Appl Mater Interfaces (2015)

SEM and STEM (insets)micrographs of FFSNPs (a) and fluorescence emission spectra of FFSNPsaqueous suspensions at 550 nm excitation and fluorescence microscopymicrographs (b). Scale bars are 200 nm, 10 nm, and 20 μm inall SEM, STEM, and fluorescence microscopy images, respectively.
© Copyright Policy - editor-choice
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490775&req=5

fig1: SEM and STEM (insets)micrographs of FFSNPs (a) and fluorescence emission spectra of FFSNPsaqueous suspensions at 550 nm excitation and fluorescence microscopymicrographs (b). Scale bars are 200 nm, 10 nm, and 20 μm inall SEM, STEM, and fluorescence microscopy images, respectively.
Mentions: SEM and STEM analysis revealed that all types of FFSNPs synthesizedare of spherical shape and show a narrow size distribution (Figure 1a) with a mean diameter of 58 nm (Table 1). The fluorescent emission spectra of the FFSNPswere measured at the excitation wavelength of RBITC (550 nm) and areshown in Figure 1b. All FFSNPs presented awell distinct fluorescence signal at the emission wavelength of RBITC.This was further confirmed by fluorescence microscopy, which indicatedthat all FFSNPs feature bright fluorescence.

Bottom Line: Irrespective of the original surface charge, serum proteins adsorbed onto the surface, neutralized the zeta potential values, and prevented the aggregation of the tailor-made FFSNPs.In contrast, in serum-containing medium, anionic FFSNPs were internalized by HOB cells more strongly, despite the similar size and surface charge of all types of protein-covered FFSNPs.Thus, at physiological condition, when the presence of proteins is inevitable, sulfonate-functionalized silica NPs are the favorite choice to achieve a desired high rate of NP internalization.

View Article: PubMed Central - PubMed

Affiliation: †Advanced Ceramics, University of Bremen, Am Biologischen Garten 2, 28359 Bremen, Germany.

ABSTRACT
To study the importance of the surface charge for cellular uptake of silica nanoparticles (NPs), we synthesized five different single- or multifunctionalized fluorescent silica NPs (FFSNPs) by introducing various ratios of amino and sulfonate groups into their surface. The zeta potential values of these FFSNPs were customized from highly positive to highly negative, while other physicochemical properties remained almost constant. Irrespective of the original surface charge, serum proteins adsorbed onto the surface, neutralized the zeta potential values, and prevented the aggregation of the tailor-made FFSNPs. Depending on the surface charge and on the absence or presence of serum, two opposite trends were found concerning the cellular uptake of FFSNPs. In the absence of serum, positively charged NPs were more strongly accumulated by human osteoblast (HOB) cells than negatively charged NPs. In contrast, in serum-containing medium, anionic FFSNPs were internalized by HOB cells more strongly, despite the similar size and surface charge of all types of protein-covered FFSNPs. Thus, at physiological condition, when the presence of proteins is inevitable, sulfonate-functionalized silica NPs are the favorite choice to achieve a desired high rate of NP internalization.

No MeSH data available.


Related in: MedlinePlus