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Do Matrix Metalloproteases and Tissue Inhibitors of Metalloproteases in Tenocytes of the Rotator Cuff Differ with Varying Donor Characteristics?

Klatte-Schulz F, Aleyt T, Pauly S, Geißler S, Gerhardt C, Scheibel M, Wildemann B - Int J Mol Sci (2015)

Bottom Line: Furthermore, the expression and secretion of TIMP-1, -2 and -3 was enhanced with age, muscle fatty infiltration and tear size.The interaction between MMPs and TIMPs is a complex process, since TIMPs are not only inhibitors, but also activators of MMPs.This study shows that MMPs and TIMPs might play an important role in degenerative tendon pathologies.

View Article: PubMed Central - PubMed

Affiliation: Julius Wolff Institute, Center for Musculoskeletal Surgery, Charité-Universitaetsmedizin Berlin, 13353 Berlin, Germany. franka.klatte@charite.de.

ABSTRACT
An imbalance between matrix metalloproteases (MMPs) and the tissue inhibitors of metalloproteases (TIMPs) may have a negative impact on the healing of rotator cuff tears. The aim of the project was to assess a possible relationship between clinical and radiographic characteristics of patients such as the age, sex, as well as the degenerative status of the tendon and the MMPs and TIMPs in their tenocyte-like cells (TLCs). TLCs were isolated from ruptured supraspinatus tendons and quantitative Real-Time PCR and ELISA was performed to analyze the expression and secretion of MMPs and TIMPs. In the present study, MMPs, mostly gelatinases and collagenases such as MMP-2, -9 and -13 showed an increased expression and protein secretion in TLCs of donors with higher age or degenerative status of the tendon. Furthermore, the expression and secretion of TIMP-1, -2 and -3 was enhanced with age, muscle fatty infiltration and tear size. The interaction between MMPs and TIMPs is a complex process, since TIMPs are not only inhibitors, but also activators of MMPs. This study shows that MMPs and TIMPs might play an important role in degenerative tendon pathologies.

No MeSH data available.


Related in: MedlinePlus

MMP and TIMP mRNA expression of all samples. Quantitative Real-Time PCR (qRT-PCR) analysis from torn RC tendons. The data represent the relative gene expression with 18S as reference gene using the ΔCt method with efficiency correction. Data are expressed as the mean ± SD (n = 30) given in logarithmic form. MMP-2, TIMP-1, -2, and -3 showed the highest expression levels, while MMP-9, -10, and -13 showed lowest expression.
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ijms-16-13141-f001: MMP and TIMP mRNA expression of all samples. Quantitative Real-Time PCR (qRT-PCR) analysis from torn RC tendons. The data represent the relative gene expression with 18S as reference gene using the ΔCt method with efficiency correction. Data are expressed as the mean ± SD (n = 30) given in logarithmic form. MMP-2, TIMP-1, -2, and -3 showed the highest expression levels, while MMP-9, -10, and -13 showed lowest expression.

Mentions: Gene expression analysis revealed MMP-2 expression to be the strongest in all TLCs, followed by MMP-3 and MMP-1. MMP-9, -10, and -13 were expressed in very low amounts, whereas MMP-10 and MMP-13 were only expressed in 26 or 28 samples, respectively. High expression levels of TIMP-1, -2 and -3 were found in all cells, while the TIMP-4 mRNA expression was much weaker (Figure 1). Due to the weak expression of MMP-9, -10 and -13, these MMPs were not analyzed on protein level. For the rest of the MMPs and TIMPs, the protein analysis of cell culture supernatants revealed a comparable pattern, where MMP-2 was the most secreted MMP and TIMP-1 and -2 the most secreted TIMPs in the cells (Figure 2). The FCS containing medium, which served as negative control, did not show detectable levels of MMPs or TIMPs in any of the ELISA analysis.


Do Matrix Metalloproteases and Tissue Inhibitors of Metalloproteases in Tenocytes of the Rotator Cuff Differ with Varying Donor Characteristics?

Klatte-Schulz F, Aleyt T, Pauly S, Geißler S, Gerhardt C, Scheibel M, Wildemann B - Int J Mol Sci (2015)

MMP and TIMP mRNA expression of all samples. Quantitative Real-Time PCR (qRT-PCR) analysis from torn RC tendons. The data represent the relative gene expression with 18S as reference gene using the ΔCt method with efficiency correction. Data are expressed as the mean ± SD (n = 30) given in logarithmic form. MMP-2, TIMP-1, -2, and -3 showed the highest expression levels, while MMP-9, -10, and -13 showed lowest expression.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490489&req=5

ijms-16-13141-f001: MMP and TIMP mRNA expression of all samples. Quantitative Real-Time PCR (qRT-PCR) analysis from torn RC tendons. The data represent the relative gene expression with 18S as reference gene using the ΔCt method with efficiency correction. Data are expressed as the mean ± SD (n = 30) given in logarithmic form. MMP-2, TIMP-1, -2, and -3 showed the highest expression levels, while MMP-9, -10, and -13 showed lowest expression.
Mentions: Gene expression analysis revealed MMP-2 expression to be the strongest in all TLCs, followed by MMP-3 and MMP-1. MMP-9, -10, and -13 were expressed in very low amounts, whereas MMP-10 and MMP-13 were only expressed in 26 or 28 samples, respectively. High expression levels of TIMP-1, -2 and -3 were found in all cells, while the TIMP-4 mRNA expression was much weaker (Figure 1). Due to the weak expression of MMP-9, -10 and -13, these MMPs were not analyzed on protein level. For the rest of the MMPs and TIMPs, the protein analysis of cell culture supernatants revealed a comparable pattern, where MMP-2 was the most secreted MMP and TIMP-1 and -2 the most secreted TIMPs in the cells (Figure 2). The FCS containing medium, which served as negative control, did not show detectable levels of MMPs or TIMPs in any of the ELISA analysis.

Bottom Line: Furthermore, the expression and secretion of TIMP-1, -2 and -3 was enhanced with age, muscle fatty infiltration and tear size.The interaction between MMPs and TIMPs is a complex process, since TIMPs are not only inhibitors, but also activators of MMPs.This study shows that MMPs and TIMPs might play an important role in degenerative tendon pathologies.

View Article: PubMed Central - PubMed

Affiliation: Julius Wolff Institute, Center for Musculoskeletal Surgery, Charité-Universitaetsmedizin Berlin, 13353 Berlin, Germany. franka.klatte@charite.de.

ABSTRACT
An imbalance between matrix metalloproteases (MMPs) and the tissue inhibitors of metalloproteases (TIMPs) may have a negative impact on the healing of rotator cuff tears. The aim of the project was to assess a possible relationship between clinical and radiographic characteristics of patients such as the age, sex, as well as the degenerative status of the tendon and the MMPs and TIMPs in their tenocyte-like cells (TLCs). TLCs were isolated from ruptured supraspinatus tendons and quantitative Real-Time PCR and ELISA was performed to analyze the expression and secretion of MMPs and TIMPs. In the present study, MMPs, mostly gelatinases and collagenases such as MMP-2, -9 and -13 showed an increased expression and protein secretion in TLCs of donors with higher age or degenerative status of the tendon. Furthermore, the expression and secretion of TIMP-1, -2 and -3 was enhanced with age, muscle fatty infiltration and tear size. The interaction between MMPs and TIMPs is a complex process, since TIMPs are not only inhibitors, but also activators of MMPs. This study shows that MMPs and TIMPs might play an important role in degenerative tendon pathologies.

No MeSH data available.


Related in: MedlinePlus