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Genome-Wide Analysis of Sorbitol Dehydrogenase (SDH) Genes and Their Differential Expression in Two Sand Pear (Pyrus pyrifolia) Fruits.

Dai M, Shi Z, Xu C - Int J Mol Sci (2015)

Bottom Line: Tissue-specific expression of these six members was observed in nine tissues or organs of sand pear, with the greatest abundance found in functional leaf petioles, followed by the flesh of young fruit.The transcript abundance of PpySDHs was higher in "Cuiguan" than in "Cuiyu", accompanied by a higher content of sugars and higher ratio of fructose to sorbitol maintained in the former cultivar at harvest.In conclusion, it was suggested that multiple members of the SDH gene family are possibly involved in sand pear fruit development and sugar accumulation and may affect both the sugar amount and sugar composition.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. daimeisong@163.com.

ABSTRACT
Through RNA-seq of a mixed fruit sample, fourteen expressed sorbitol dehydrogenase (SDH) genes have been identified from sand pear (Pyrus pyrifolia Nakai). Comparative phylogenetic analysis of these PpySDHs with those from other plants supported the closest relationship of sand pear with Chinese white pear (P. bretschneideri). The expression levels varied greatly among members, and the strongest six (PpySDH2, PpySDH4, PpySDH8, PpySDH12, PpySDH13 and PpySDH14) accounted for 96% of total transcript abundance of PpySDHs. Tissue-specific expression of these six members was observed in nine tissues or organs of sand pear, with the greatest abundance found in functional leaf petioles, followed by the flesh of young fruit. Expression patterns of these six PpySDH genes during fruit development were analyzed in two sand pear cultivars, "Cuiguan" and "Cuiyu". Overall, expression of PpySDHs peaked twice, first at the fruitlet stage and again at or near harvest. The transcript abundance of PpySDHs was higher in "Cuiguan" than in "Cuiyu", accompanied by a higher content of sugars and higher ratio of fructose to sorbitol maintained in the former cultivar at harvest. In conclusion, it was suggested that multiple members of the SDH gene family are possibly involved in sand pear fruit development and sugar accumulation and may affect both the sugar amount and sugar composition.

No MeSH data available.


Relative expression levels of six selected PpySDH genes in nine tissues of sand pear (P. pyrifolia Nakai. cv. Cuiyu). The expression of each gene was first normalized against 18S rRNA (internal control) by using the 2−ΔCt method, and then, the strongest expression, PpySDH2 in functional leaf petiole in this figure, served as a calibrator (1.0); expression of the remaining genes was expressed as a multiple of the calibrator. Error bars indicate the standard error (SE) from three biological replicates. Different letters within each chart indicate means that are significantly different (p ≤ 0.05).
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ijms-16-13065-f003: Relative expression levels of six selected PpySDH genes in nine tissues of sand pear (P. pyrifolia Nakai. cv. Cuiyu). The expression of each gene was first normalized against 18S rRNA (internal control) by using the 2−ΔCt method, and then, the strongest expression, PpySDH2 in functional leaf petiole in this figure, served as a calibrator (1.0); expression of the remaining genes was expressed as a multiple of the calibrator. Error bars indicate the standard error (SE) from three biological replicates. Different letters within each chart indicate means that are significantly different (p ≤ 0.05).

Mentions: Tissue-specific expression patterns of the above-mentioned six most strongly-expressed PpySDHs were analyzed with nine tissues or organs of “Cuiyu” sand pear. The strongest expression was observed for PpySDH2 in functional leaf petiole, followed by several other members in leaf petiole tissue (Figure 3). Expression of four members, PpySDH2, PpySDH4, PpySDH12 and PpySDH14, was also high in young flesh tissues. In mature fruit flesh, PpySDH4 was the main expressed member. Expression was relatively low in functional leaves, young leaves and seeds for all six members analyzed (Figure 3).


Genome-Wide Analysis of Sorbitol Dehydrogenase (SDH) Genes and Their Differential Expression in Two Sand Pear (Pyrus pyrifolia) Fruits.

Dai M, Shi Z, Xu C - Int J Mol Sci (2015)

Relative expression levels of six selected PpySDH genes in nine tissues of sand pear (P. pyrifolia Nakai. cv. Cuiyu). The expression of each gene was first normalized against 18S rRNA (internal control) by using the 2−ΔCt method, and then, the strongest expression, PpySDH2 in functional leaf petiole in this figure, served as a calibrator (1.0); expression of the remaining genes was expressed as a multiple of the calibrator. Error bars indicate the standard error (SE) from three biological replicates. Different letters within each chart indicate means that are significantly different (p ≤ 0.05).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490486&req=5

ijms-16-13065-f003: Relative expression levels of six selected PpySDH genes in nine tissues of sand pear (P. pyrifolia Nakai. cv. Cuiyu). The expression of each gene was first normalized against 18S rRNA (internal control) by using the 2−ΔCt method, and then, the strongest expression, PpySDH2 in functional leaf petiole in this figure, served as a calibrator (1.0); expression of the remaining genes was expressed as a multiple of the calibrator. Error bars indicate the standard error (SE) from three biological replicates. Different letters within each chart indicate means that are significantly different (p ≤ 0.05).
Mentions: Tissue-specific expression patterns of the above-mentioned six most strongly-expressed PpySDHs were analyzed with nine tissues or organs of “Cuiyu” sand pear. The strongest expression was observed for PpySDH2 in functional leaf petiole, followed by several other members in leaf petiole tissue (Figure 3). Expression of four members, PpySDH2, PpySDH4, PpySDH12 and PpySDH14, was also high in young flesh tissues. In mature fruit flesh, PpySDH4 was the main expressed member. Expression was relatively low in functional leaves, young leaves and seeds for all six members analyzed (Figure 3).

Bottom Line: Tissue-specific expression of these six members was observed in nine tissues or organs of sand pear, with the greatest abundance found in functional leaf petioles, followed by the flesh of young fruit.The transcript abundance of PpySDHs was higher in "Cuiguan" than in "Cuiyu", accompanied by a higher content of sugars and higher ratio of fructose to sorbitol maintained in the former cultivar at harvest.In conclusion, it was suggested that multiple members of the SDH gene family are possibly involved in sand pear fruit development and sugar accumulation and may affect both the sugar amount and sugar composition.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. daimeisong@163.com.

ABSTRACT
Through RNA-seq of a mixed fruit sample, fourteen expressed sorbitol dehydrogenase (SDH) genes have been identified from sand pear (Pyrus pyrifolia Nakai). Comparative phylogenetic analysis of these PpySDHs with those from other plants supported the closest relationship of sand pear with Chinese white pear (P. bretschneideri). The expression levels varied greatly among members, and the strongest six (PpySDH2, PpySDH4, PpySDH8, PpySDH12, PpySDH13 and PpySDH14) accounted for 96% of total transcript abundance of PpySDHs. Tissue-specific expression of these six members was observed in nine tissues or organs of sand pear, with the greatest abundance found in functional leaf petioles, followed by the flesh of young fruit. Expression patterns of these six PpySDH genes during fruit development were analyzed in two sand pear cultivars, "Cuiguan" and "Cuiyu". Overall, expression of PpySDHs peaked twice, first at the fruitlet stage and again at or near harvest. The transcript abundance of PpySDHs was higher in "Cuiguan" than in "Cuiyu", accompanied by a higher content of sugars and higher ratio of fructose to sorbitol maintained in the former cultivar at harvest. In conclusion, it was suggested that multiple members of the SDH gene family are possibly involved in sand pear fruit development and sugar accumulation and may affect both the sugar amount and sugar composition.

No MeSH data available.