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Genome-Wide Analysis of Sorbitol Dehydrogenase (SDH) Genes and Their Differential Expression in Two Sand Pear (Pyrus pyrifolia) Fruits.

Dai M, Shi Z, Xu C - Int J Mol Sci (2015)

Bottom Line: Tissue-specific expression of these six members was observed in nine tissues or organs of sand pear, with the greatest abundance found in functional leaf petioles, followed by the flesh of young fruit.The transcript abundance of PpySDHs was higher in "Cuiguan" than in "Cuiyu", accompanied by a higher content of sugars and higher ratio of fructose to sorbitol maintained in the former cultivar at harvest.In conclusion, it was suggested that multiple members of the SDH gene family are possibly involved in sand pear fruit development and sugar accumulation and may affect both the sugar amount and sugar composition.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. daimeisong@163.com.

ABSTRACT
Through RNA-seq of a mixed fruit sample, fourteen expressed sorbitol dehydrogenase (SDH) genes have been identified from sand pear (Pyrus pyrifolia Nakai). Comparative phylogenetic analysis of these PpySDHs with those from other plants supported the closest relationship of sand pear with Chinese white pear (P. bretschneideri). The expression levels varied greatly among members, and the strongest six (PpySDH2, PpySDH4, PpySDH8, PpySDH12, PpySDH13 and PpySDH14) accounted for 96% of total transcript abundance of PpySDHs. Tissue-specific expression of these six members was observed in nine tissues or organs of sand pear, with the greatest abundance found in functional leaf petioles, followed by the flesh of young fruit. Expression patterns of these six PpySDH genes during fruit development were analyzed in two sand pear cultivars, "Cuiguan" and "Cuiyu". Overall, expression of PpySDHs peaked twice, first at the fruitlet stage and again at or near harvest. The transcript abundance of PpySDHs was higher in "Cuiguan" than in "Cuiyu", accompanied by a higher content of sugars and higher ratio of fructose to sorbitol maintained in the former cultivar at harvest. In conclusion, it was suggested that multiple members of the SDH gene family are possibly involved in sand pear fruit development and sugar accumulation and may affect both the sugar amount and sugar composition.

No MeSH data available.


Phylogenetic tree of 83 SDH homologous genes from 12 plant species, including 14 PpySDH genes (●) identified from the P. pyrifolia transcriptome. The phylogenetic tree was generated using the neighbor joining (NJ) method in Molecular Evolutionary Genetics Analysis version 6 (MEGA6) [19]. Numbers given at branch nodes are the bootstrap values of the confidence level, as percentages calculated from 1000 random replications.
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ijms-16-13065-f001: Phylogenetic tree of 83 SDH homologous genes from 12 plant species, including 14 PpySDH genes (●) identified from the P. pyrifolia transcriptome. The phylogenetic tree was generated using the neighbor joining (NJ) method in Molecular Evolutionary Genetics Analysis version 6 (MEGA6) [19]. Numbers given at branch nodes are the bootstrap values of the confidence level, as percentages calculated from 1000 random replications.

Mentions: In order to investigate phylogenetic relationships and the molecular evolutionary history of the sequences, each member of the plant SDH family was used to construct the phylogenetic tree by using the neighbor joining (NJ) method in Molecular Evolutionary Genetics Analysis version 6 (MEGA6) [19]. All SDH genes fall into three major clades, with Group 1 containing most of the SDH genes from Rosaceae species. Group 2 and Group 3 each have only two genes from a single species (Figure 1).


Genome-Wide Analysis of Sorbitol Dehydrogenase (SDH) Genes and Their Differential Expression in Two Sand Pear (Pyrus pyrifolia) Fruits.

Dai M, Shi Z, Xu C - Int J Mol Sci (2015)

Phylogenetic tree of 83 SDH homologous genes from 12 plant species, including 14 PpySDH genes (●) identified from the P. pyrifolia transcriptome. The phylogenetic tree was generated using the neighbor joining (NJ) method in Molecular Evolutionary Genetics Analysis version 6 (MEGA6) [19]. Numbers given at branch nodes are the bootstrap values of the confidence level, as percentages calculated from 1000 random replications.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490486&req=5

ijms-16-13065-f001: Phylogenetic tree of 83 SDH homologous genes from 12 plant species, including 14 PpySDH genes (●) identified from the P. pyrifolia transcriptome. The phylogenetic tree was generated using the neighbor joining (NJ) method in Molecular Evolutionary Genetics Analysis version 6 (MEGA6) [19]. Numbers given at branch nodes are the bootstrap values of the confidence level, as percentages calculated from 1000 random replications.
Mentions: In order to investigate phylogenetic relationships and the molecular evolutionary history of the sequences, each member of the plant SDH family was used to construct the phylogenetic tree by using the neighbor joining (NJ) method in Molecular Evolutionary Genetics Analysis version 6 (MEGA6) [19]. All SDH genes fall into three major clades, with Group 1 containing most of the SDH genes from Rosaceae species. Group 2 and Group 3 each have only two genes from a single species (Figure 1).

Bottom Line: Tissue-specific expression of these six members was observed in nine tissues or organs of sand pear, with the greatest abundance found in functional leaf petioles, followed by the flesh of young fruit.The transcript abundance of PpySDHs was higher in "Cuiguan" than in "Cuiyu", accompanied by a higher content of sugars and higher ratio of fructose to sorbitol maintained in the former cultivar at harvest.In conclusion, it was suggested that multiple members of the SDH gene family are possibly involved in sand pear fruit development and sugar accumulation and may affect both the sugar amount and sugar composition.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. daimeisong@163.com.

ABSTRACT
Through RNA-seq of a mixed fruit sample, fourteen expressed sorbitol dehydrogenase (SDH) genes have been identified from sand pear (Pyrus pyrifolia Nakai). Comparative phylogenetic analysis of these PpySDHs with those from other plants supported the closest relationship of sand pear with Chinese white pear (P. bretschneideri). The expression levels varied greatly among members, and the strongest six (PpySDH2, PpySDH4, PpySDH8, PpySDH12, PpySDH13 and PpySDH14) accounted for 96% of total transcript abundance of PpySDHs. Tissue-specific expression of these six members was observed in nine tissues or organs of sand pear, with the greatest abundance found in functional leaf petioles, followed by the flesh of young fruit. Expression patterns of these six PpySDH genes during fruit development were analyzed in two sand pear cultivars, "Cuiguan" and "Cuiyu". Overall, expression of PpySDHs peaked twice, first at the fruitlet stage and again at or near harvest. The transcript abundance of PpySDHs was higher in "Cuiguan" than in "Cuiyu", accompanied by a higher content of sugars and higher ratio of fructose to sorbitol maintained in the former cultivar at harvest. In conclusion, it was suggested that multiple members of the SDH gene family are possibly involved in sand pear fruit development and sugar accumulation and may affect both the sugar amount and sugar composition.

No MeSH data available.