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Characterization of a Bioflocculant (MBF-UFH) Produced by Bacillus sp. AEMREG7.

Okaiyeto K, Nwodo UU, Mabinya LV, Okoli AS, Okoh AI - Int J Mol Sci (2015)

Bottom Line: The production of the bioflocculant was observed to be closely associated with cell growth.The bioflocculant retained high flocculating activity of 90% after heating at 100 °C for 1 h.MBF-UFH appears to have immense potential as an alternative to conventional chemical flocculants.

View Article: PubMed Central - PubMed

Affiliation: South Africa-Medical Research Council (SA-MRC), Microbial Water Quality Monitoring Centre, University of Fort Hare, 5700 Alice, South Africa. okaiyetofranciskunle@yahoo.ca.

ABSTRACT
A bioflocculant named MBF-UFH produced by a Bacillus species isolated from sediment samples of Algoa Bay of the Eastern Cape Province of South Africa was characterized. The bacterial identification was through 16S rDNA sequencing; nucleotide sequences were deposited in GenBank as Bacillus sp. AEMREG7 with Accession Number KP659187. The production of the bioflocculant was observed to be closely associated with cell growth. The bioflocculant had the highest flocculating activity of 83.2% after 72 h of cultivation, and approximately 1.6 g of purified MBF-UFH was recovered from 1 L of fermentation broth. Its chemical analyses indicated that it is a glycoprotein composed of polysaccharide (76%) and protein (14%). Fourier transform infrared spectroscopy (FTIR) revealed that it consisted of hydroxyl, amide, carboxyl and methoxyl as the functional moieties. Scanning electron microscopy (SEM) revealed the amorphous structure of MBF-UFH and flocculated kaolin clay particles. The maximum flocculating activity of 92.6% against kaolin clay suspension was achieved at 0.3 mg/mL over pH ranges of 3-11 with the peak flocculating rate at pH 8 in the presence of MgCl2. The bioflocculant retained high flocculating activity of 90% after heating at 100 °C for 1 h. MBF-UFH appears to have immense potential as an alternative to conventional chemical flocculants.

No MeSH data available.


Thermal stability of purified MBF-UFH produced by Bacillus sp. AEMREG7.
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ijms-16-12986-f006: Thermal stability of purified MBF-UFH produced by Bacillus sp. AEMREG7.

Mentions: The thermal stability of the purified bioflocculant was examined at 50, 60, 70, 80, 90 and 100 °C for 1 h at each temperature. As depicted in Figure 6, MBF-UFH had an exceptional flocculating activity of 90% over all of the temperature regimes. The high flocculating efficiency showed by MBF-UFH at 100 °C indicated that it is thermally stable and consisted predominantly of polysaccharide [46,47]. The thermal stability may be due to the presence of a hydroxyl group involved in the formation of hydrogen bonds in MBF-UFH structure [44]. The stability of MBF-UFH was similar to the bioflocculant produced by C. glutamicum, which retained high flocculating activity of 96.9% at 80 °C, but the stability decreased slightly on increasing the temperature to 100 °C [47]. Besides, the bioflocculant produced by Aspergillus flavus was thermally stable, which retained high flocculating activity above 90% over a temperature range of 10–100 °C [24]. Nevertheless, Salehizadel et al. [48] reported a less stable bioflocculant that lost about 50% of the flocculating activity after heating for 15 min at 100 °C.


Characterization of a Bioflocculant (MBF-UFH) Produced by Bacillus sp. AEMREG7.

Okaiyeto K, Nwodo UU, Mabinya LV, Okoli AS, Okoh AI - Int J Mol Sci (2015)

Thermal stability of purified MBF-UFH produced by Bacillus sp. AEMREG7.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490482&req=5

ijms-16-12986-f006: Thermal stability of purified MBF-UFH produced by Bacillus sp. AEMREG7.
Mentions: The thermal stability of the purified bioflocculant was examined at 50, 60, 70, 80, 90 and 100 °C for 1 h at each temperature. As depicted in Figure 6, MBF-UFH had an exceptional flocculating activity of 90% over all of the temperature regimes. The high flocculating efficiency showed by MBF-UFH at 100 °C indicated that it is thermally stable and consisted predominantly of polysaccharide [46,47]. The thermal stability may be due to the presence of a hydroxyl group involved in the formation of hydrogen bonds in MBF-UFH structure [44]. The stability of MBF-UFH was similar to the bioflocculant produced by C. glutamicum, which retained high flocculating activity of 96.9% at 80 °C, but the stability decreased slightly on increasing the temperature to 100 °C [47]. Besides, the bioflocculant produced by Aspergillus flavus was thermally stable, which retained high flocculating activity above 90% over a temperature range of 10–100 °C [24]. Nevertheless, Salehizadel et al. [48] reported a less stable bioflocculant that lost about 50% of the flocculating activity after heating for 15 min at 100 °C.

Bottom Line: The production of the bioflocculant was observed to be closely associated with cell growth.The bioflocculant retained high flocculating activity of 90% after heating at 100 °C for 1 h.MBF-UFH appears to have immense potential as an alternative to conventional chemical flocculants.

View Article: PubMed Central - PubMed

Affiliation: South Africa-Medical Research Council (SA-MRC), Microbial Water Quality Monitoring Centre, University of Fort Hare, 5700 Alice, South Africa. okaiyetofranciskunle@yahoo.ca.

ABSTRACT
A bioflocculant named MBF-UFH produced by a Bacillus species isolated from sediment samples of Algoa Bay of the Eastern Cape Province of South Africa was characterized. The bacterial identification was through 16S rDNA sequencing; nucleotide sequences were deposited in GenBank as Bacillus sp. AEMREG7 with Accession Number KP659187. The production of the bioflocculant was observed to be closely associated with cell growth. The bioflocculant had the highest flocculating activity of 83.2% after 72 h of cultivation, and approximately 1.6 g of purified MBF-UFH was recovered from 1 L of fermentation broth. Its chemical analyses indicated that it is a glycoprotein composed of polysaccharide (76%) and protein (14%). Fourier transform infrared spectroscopy (FTIR) revealed that it consisted of hydroxyl, amide, carboxyl and methoxyl as the functional moieties. Scanning electron microscopy (SEM) revealed the amorphous structure of MBF-UFH and flocculated kaolin clay particles. The maximum flocculating activity of 92.6% against kaolin clay suspension was achieved at 0.3 mg/mL over pH ranges of 3-11 with the peak flocculating rate at pH 8 in the presence of MgCl2. The bioflocculant retained high flocculating activity of 90% after heating at 100 °C for 1 h. MBF-UFH appears to have immense potential as an alternative to conventional chemical flocculants.

No MeSH data available.