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Characterization of a Bioflocculant (MBF-UFH) Produced by Bacillus sp. AEMREG7.

Okaiyeto K, Nwodo UU, Mabinya LV, Okoli AS, Okoh AI - Int J Mol Sci (2015)

Bottom Line: The production of the bioflocculant was observed to be closely associated with cell growth.The bioflocculant retained high flocculating activity of 90% after heating at 100 °C for 1 h.MBF-UFH appears to have immense potential as an alternative to conventional chemical flocculants.

View Article: PubMed Central - PubMed

Affiliation: South Africa-Medical Research Council (SA-MRC), Microbial Water Quality Monitoring Centre, University of Fort Hare, 5700 Alice, South Africa. okaiyetofranciskunle@yahoo.ca.

ABSTRACT
A bioflocculant named MBF-UFH produced by a Bacillus species isolated from sediment samples of Algoa Bay of the Eastern Cape Province of South Africa was characterized. The bacterial identification was through 16S rDNA sequencing; nucleotide sequences were deposited in GenBank as Bacillus sp. AEMREG7 with Accession Number KP659187. The production of the bioflocculant was observed to be closely associated with cell growth. The bioflocculant had the highest flocculating activity of 83.2% after 72 h of cultivation, and approximately 1.6 g of purified MBF-UFH was recovered from 1 L of fermentation broth. Its chemical analyses indicated that it is a glycoprotein composed of polysaccharide (76%) and protein (14%). Fourier transform infrared spectroscopy (FTIR) revealed that it consisted of hydroxyl, amide, carboxyl and methoxyl as the functional moieties. Scanning electron microscopy (SEM) revealed the amorphous structure of MBF-UFH and flocculated kaolin clay particles. The maximum flocculating activity of 92.6% against kaolin clay suspension was achieved at 0.3 mg/mL over pH ranges of 3-11 with the peak flocculating rate at pH 8 in the presence of MgCl2. The bioflocculant retained high flocculating activity of 90% after heating at 100 °C for 1 h. MBF-UFH appears to have immense potential as an alternative to conventional chemical flocculants.

No MeSH data available.


SEM imaging of purified MBF-UFH produced by Bacillus sp. AEMREG7 (A), kaolin clay particles (B) and kaolin clay suspension flocculated with MBF-UFH (C).
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ijms-16-12986-f003: SEM imaging of purified MBF-UFH produced by Bacillus sp. AEMREG7 (A), kaolin clay particles (B) and kaolin clay suspension flocculated with MBF-UFH (C).

Mentions: Surface morphology structure of the bioflocculant was explicated by scanning electron microscopy (SEM). SEM is a type of electron microscope that divulges the image of a sample by scanning it with a high-energy beam of electrons in a raster scan pattern [25]. The electrons interrelate with the atoms that make up the sample, producing signals that contain information about the sample’s surface structure [33]. The SEM image showed that MBF-UFH has an amorphous structure of a compact nature (Figure 3A). The configuration of this bioflocculant may be accountable for its high flocculation efficiency. Before the flocculation process, the kaolin clay particles appeared to be fine and scattered (Figure 3B), and after the flocculation process, the functional moieties in the molecular chain of MBF-UFH were used for attachment on the kaolin clay particle. Consequently, the interaction between the bioflocculant and kaolin clay particle resulted in the formation of flocs that later aggregated to larger sized flocs, which precipitated out of the suspension as the result of gravity (Figure 3C). This observation showed that bridging played a vital role in the flocculation process [34]. These results concur with previous findings for the purified bioflocculants produced by a consortium of Streptomyces and Cellulomonas species and Nocardiopsis aegyptia sp. nov. [6,17].


Characterization of a Bioflocculant (MBF-UFH) Produced by Bacillus sp. AEMREG7.

Okaiyeto K, Nwodo UU, Mabinya LV, Okoli AS, Okoh AI - Int J Mol Sci (2015)

SEM imaging of purified MBF-UFH produced by Bacillus sp. AEMREG7 (A), kaolin clay particles (B) and kaolin clay suspension flocculated with MBF-UFH (C).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490482&req=5

ijms-16-12986-f003: SEM imaging of purified MBF-UFH produced by Bacillus sp. AEMREG7 (A), kaolin clay particles (B) and kaolin clay suspension flocculated with MBF-UFH (C).
Mentions: Surface morphology structure of the bioflocculant was explicated by scanning electron microscopy (SEM). SEM is a type of electron microscope that divulges the image of a sample by scanning it with a high-energy beam of electrons in a raster scan pattern [25]. The electrons interrelate with the atoms that make up the sample, producing signals that contain information about the sample’s surface structure [33]. The SEM image showed that MBF-UFH has an amorphous structure of a compact nature (Figure 3A). The configuration of this bioflocculant may be accountable for its high flocculation efficiency. Before the flocculation process, the kaolin clay particles appeared to be fine and scattered (Figure 3B), and after the flocculation process, the functional moieties in the molecular chain of MBF-UFH were used for attachment on the kaolin clay particle. Consequently, the interaction between the bioflocculant and kaolin clay particle resulted in the formation of flocs that later aggregated to larger sized flocs, which precipitated out of the suspension as the result of gravity (Figure 3C). This observation showed that bridging played a vital role in the flocculation process [34]. These results concur with previous findings for the purified bioflocculants produced by a consortium of Streptomyces and Cellulomonas species and Nocardiopsis aegyptia sp. nov. [6,17].

Bottom Line: The production of the bioflocculant was observed to be closely associated with cell growth.The bioflocculant retained high flocculating activity of 90% after heating at 100 °C for 1 h.MBF-UFH appears to have immense potential as an alternative to conventional chemical flocculants.

View Article: PubMed Central - PubMed

Affiliation: South Africa-Medical Research Council (SA-MRC), Microbial Water Quality Monitoring Centre, University of Fort Hare, 5700 Alice, South Africa. okaiyetofranciskunle@yahoo.ca.

ABSTRACT
A bioflocculant named MBF-UFH produced by a Bacillus species isolated from sediment samples of Algoa Bay of the Eastern Cape Province of South Africa was characterized. The bacterial identification was through 16S rDNA sequencing; nucleotide sequences were deposited in GenBank as Bacillus sp. AEMREG7 with Accession Number KP659187. The production of the bioflocculant was observed to be closely associated with cell growth. The bioflocculant had the highest flocculating activity of 83.2% after 72 h of cultivation, and approximately 1.6 g of purified MBF-UFH was recovered from 1 L of fermentation broth. Its chemical analyses indicated that it is a glycoprotein composed of polysaccharide (76%) and protein (14%). Fourier transform infrared spectroscopy (FTIR) revealed that it consisted of hydroxyl, amide, carboxyl and methoxyl as the functional moieties. Scanning electron microscopy (SEM) revealed the amorphous structure of MBF-UFH and flocculated kaolin clay particles. The maximum flocculating activity of 92.6% against kaolin clay suspension was achieved at 0.3 mg/mL over pH ranges of 3-11 with the peak flocculating rate at pH 8 in the presence of MgCl2. The bioflocculant retained high flocculating activity of 90% after heating at 100 °C for 1 h. MBF-UFH appears to have immense potential as an alternative to conventional chemical flocculants.

No MeSH data available.