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Characterization of a Bioflocculant (MBF-UFH) Produced by Bacillus sp. AEMREG7.

Okaiyeto K, Nwodo UU, Mabinya LV, Okoli AS, Okoh AI - Int J Mol Sci (2015)

Bottom Line: The production of the bioflocculant was observed to be closely associated with cell growth.The bioflocculant retained high flocculating activity of 90% after heating at 100 °C for 1 h.MBF-UFH appears to have immense potential as an alternative to conventional chemical flocculants.

View Article: PubMed Central - PubMed

Affiliation: South Africa-Medical Research Council (SA-MRC), Microbial Water Quality Monitoring Centre, University of Fort Hare, 5700 Alice, South Africa. okaiyetofranciskunle@yahoo.ca.

ABSTRACT
A bioflocculant named MBF-UFH produced by a Bacillus species isolated from sediment samples of Algoa Bay of the Eastern Cape Province of South Africa was characterized. The bacterial identification was through 16S rDNA sequencing; nucleotide sequences were deposited in GenBank as Bacillus sp. AEMREG7 with Accession Number KP659187. The production of the bioflocculant was observed to be closely associated with cell growth. The bioflocculant had the highest flocculating activity of 83.2% after 72 h of cultivation, and approximately 1.6 g of purified MBF-UFH was recovered from 1 L of fermentation broth. Its chemical analyses indicated that it is a glycoprotein composed of polysaccharide (76%) and protein (14%). Fourier transform infrared spectroscopy (FTIR) revealed that it consisted of hydroxyl, amide, carboxyl and methoxyl as the functional moieties. Scanning electron microscopy (SEM) revealed the amorphous structure of MBF-UFH and flocculated kaolin clay particles. The maximum flocculating activity of 92.6% against kaolin clay suspension was achieved at 0.3 mg/mL over pH ranges of 3-11 with the peak flocculating rate at pH 8 in the presence of MgCl2. The bioflocculant retained high flocculating activity of 90% after heating at 100 °C for 1 h. MBF-UFH appears to have immense potential as an alternative to conventional chemical flocculants.

No MeSH data available.


Fourier transform infrared (FTIR) spectra of purified MBF-UFH produced by Bacillus sp. AEMREG7.
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ijms-16-12986-f002: Fourier transform infrared (FTIR) spectra of purified MBF-UFH produced by Bacillus sp. AEMREG7.

Mentions: The functional moieties in the molecular chain of MBF-UFH were identified with FTIR spectrophotometry (Figure 2). The spectrum displaced an intense broad stretching peak at 3440 cm−1, which indicated the presence of a hydroxyl or amide group [25,26]. The water solubility of the bioflocculant was attributed to the presence of hydroxyl group forming a hydrogen bond with a water molecule. A weak peak observed at 2367 cm−1 was either CO2 adsorption or may be from the amine group [27]. Furthermore, an asymmetric stretching peak was at 1638 cm−1, which showed the presence of carbonyl group stretching vibration in the peptide [28]. The peak detected at 1412 cm−1 could be ascribed to the symmetric stretching of the –COO− group [18]. The presence of carboxyl groups provides more adsorption sites for particle attachment, so many particles can be adsorbed to the long molecular chain of the bioflocculant [29]. The absorption peaks ranging from 1000–1200 cm−1 were designated to C–O–C and C–O, which indicated the presence of polysaccharides [30,31]. The peak at 1077 cm−1 indicated the presence of methoxyl groups [9]. The characteristics of the FTIR spectrum showed the presence of hydroxyl, amide, carboxyl and methoxyl groups in the MBF-UFH structure as the main functional moieties preferred for flocculation [32].


Characterization of a Bioflocculant (MBF-UFH) Produced by Bacillus sp. AEMREG7.

Okaiyeto K, Nwodo UU, Mabinya LV, Okoli AS, Okoh AI - Int J Mol Sci (2015)

Fourier transform infrared (FTIR) spectra of purified MBF-UFH produced by Bacillus sp. AEMREG7.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490482&req=5

ijms-16-12986-f002: Fourier transform infrared (FTIR) spectra of purified MBF-UFH produced by Bacillus sp. AEMREG7.
Mentions: The functional moieties in the molecular chain of MBF-UFH were identified with FTIR spectrophotometry (Figure 2). The spectrum displaced an intense broad stretching peak at 3440 cm−1, which indicated the presence of a hydroxyl or amide group [25,26]. The water solubility of the bioflocculant was attributed to the presence of hydroxyl group forming a hydrogen bond with a water molecule. A weak peak observed at 2367 cm−1 was either CO2 adsorption or may be from the amine group [27]. Furthermore, an asymmetric stretching peak was at 1638 cm−1, which showed the presence of carbonyl group stretching vibration in the peptide [28]. The peak detected at 1412 cm−1 could be ascribed to the symmetric stretching of the –COO− group [18]. The presence of carboxyl groups provides more adsorption sites for particle attachment, so many particles can be adsorbed to the long molecular chain of the bioflocculant [29]. The absorption peaks ranging from 1000–1200 cm−1 were designated to C–O–C and C–O, which indicated the presence of polysaccharides [30,31]. The peak at 1077 cm−1 indicated the presence of methoxyl groups [9]. The characteristics of the FTIR spectrum showed the presence of hydroxyl, amide, carboxyl and methoxyl groups in the MBF-UFH structure as the main functional moieties preferred for flocculation [32].

Bottom Line: The production of the bioflocculant was observed to be closely associated with cell growth.The bioflocculant retained high flocculating activity of 90% after heating at 100 °C for 1 h.MBF-UFH appears to have immense potential as an alternative to conventional chemical flocculants.

View Article: PubMed Central - PubMed

Affiliation: South Africa-Medical Research Council (SA-MRC), Microbial Water Quality Monitoring Centre, University of Fort Hare, 5700 Alice, South Africa. okaiyetofranciskunle@yahoo.ca.

ABSTRACT
A bioflocculant named MBF-UFH produced by a Bacillus species isolated from sediment samples of Algoa Bay of the Eastern Cape Province of South Africa was characterized. The bacterial identification was through 16S rDNA sequencing; nucleotide sequences were deposited in GenBank as Bacillus sp. AEMREG7 with Accession Number KP659187. The production of the bioflocculant was observed to be closely associated with cell growth. The bioflocculant had the highest flocculating activity of 83.2% after 72 h of cultivation, and approximately 1.6 g of purified MBF-UFH was recovered from 1 L of fermentation broth. Its chemical analyses indicated that it is a glycoprotein composed of polysaccharide (76%) and protein (14%). Fourier transform infrared spectroscopy (FTIR) revealed that it consisted of hydroxyl, amide, carboxyl and methoxyl as the functional moieties. Scanning electron microscopy (SEM) revealed the amorphous structure of MBF-UFH and flocculated kaolin clay particles. The maximum flocculating activity of 92.6% against kaolin clay suspension was achieved at 0.3 mg/mL over pH ranges of 3-11 with the peak flocculating rate at pH 8 in the presence of MgCl2. The bioflocculant retained high flocculating activity of 90% after heating at 100 °C for 1 h. MBF-UFH appears to have immense potential as an alternative to conventional chemical flocculants.

No MeSH data available.