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The Effect of Growth Hormone Administration on the Regulation of Mitochondrial Apoptosis in-Vivo.

Keane J, Tajouri L, Gray B - Int J Mol Sci (2015)

Bottom Line: Results showed that rhGH significantly decreased Bak protein concentrations compared to placebo samples for up to 8 days post treatment.While cytosolic miRNA expression was not found to be significantly affected by rhGH, measurement of the expression of miR-125b in mitochondrial fractions showed a significant down-regulation eight days post-rhGH administration.These findings suggest that rhGH induces short-term anti-apoptotic effects which may be partially mediated through a novel pathway that alters the concentration of mitochondrially-associated miRNAs.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Health Science and Medicine, Bond University, Gold Coast, Queensland 4227, Australia. james.keane@alumni.bond.edu.au.

ABSTRACT
The purpose of this study was to determine whether recombinant human growth hormone (rhGH) would show any significant effects on the expression of apoptosis regulating proteins in peripheral blood mononuclear cells (PBMCs). Additionally, the potential for post-transcriptional regulation of gene expression by miRNA was assessed in two cellular compartments, the cytosol and the mitochondria. Ten male subjects were subcutaneously injected with either rhGH (1 mg) or saline (0.9%) for seven consecutive days in a double-blinded fashion. Blood sampling was undertaken prior to treatment administration and over a period of three weeks following treatment cessation. Bcl-2 and Bak gene and protein expression levels were measured in PBMCs, while attention was also directed to the expression of miR-181a and miR-125b, known translational inhibitors of Bcl-2 and Bak respectively. Results showed that rhGH significantly decreased Bak protein concentrations compared to placebo samples for up to 8 days post treatment. While cytosolic miRNA expression was not found to be significantly affected by rhGH, measurement of the expression of miR-125b in mitochondrial fractions showed a significant down-regulation eight days post-rhGH administration. These findings suggest that rhGH induces short-term anti-apoptotic effects which may be partially mediated through a novel pathway that alters the concentration of mitochondrially-associated miRNAs.

No MeSH data available.


(A) Changes from baseline measurements in the expression of Bak mRNA levels from PBMCs in rhGH treated compared to placebo treated samples; (B) Changes from baseline measurements in the expression of Bcl-2 mRNA levels from PBMCs in rhGH treated compared to placebo treated samples.
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ijms-16-12753-f002: (A) Changes from baseline measurements in the expression of Bak mRNA levels from PBMCs in rhGH treated compared to placebo treated samples; (B) Changes from baseline measurements in the expression of Bcl-2 mRNA levels from PBMCs in rhGH treated compared to placebo treated samples.

Mentions: Seven days of administration of rhGH (1 mg per day) was not found to exhibit any significant effect on Bak mRNA differences in expression from baseline levels for any measured time period (1, 8, 15 and 22 days following cessation of the treatment program) compared to placebo treated controls (Figure 2A). Similarly, rhGH treated samples were not found to have any significant effect on Bcl-2 mRNA changes in expression from baseline levels in comparison to placebo treated samples at any time point analysed post treatment (Figure 2B).


The Effect of Growth Hormone Administration on the Regulation of Mitochondrial Apoptosis in-Vivo.

Keane J, Tajouri L, Gray B - Int J Mol Sci (2015)

(A) Changes from baseline measurements in the expression of Bak mRNA levels from PBMCs in rhGH treated compared to placebo treated samples; (B) Changes from baseline measurements in the expression of Bcl-2 mRNA levels from PBMCs in rhGH treated compared to placebo treated samples.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490471&req=5

ijms-16-12753-f002: (A) Changes from baseline measurements in the expression of Bak mRNA levels from PBMCs in rhGH treated compared to placebo treated samples; (B) Changes from baseline measurements in the expression of Bcl-2 mRNA levels from PBMCs in rhGH treated compared to placebo treated samples.
Mentions: Seven days of administration of rhGH (1 mg per day) was not found to exhibit any significant effect on Bak mRNA differences in expression from baseline levels for any measured time period (1, 8, 15 and 22 days following cessation of the treatment program) compared to placebo treated controls (Figure 2A). Similarly, rhGH treated samples were not found to have any significant effect on Bcl-2 mRNA changes in expression from baseline levels in comparison to placebo treated samples at any time point analysed post treatment (Figure 2B).

Bottom Line: Results showed that rhGH significantly decreased Bak protein concentrations compared to placebo samples for up to 8 days post treatment.While cytosolic miRNA expression was not found to be significantly affected by rhGH, measurement of the expression of miR-125b in mitochondrial fractions showed a significant down-regulation eight days post-rhGH administration.These findings suggest that rhGH induces short-term anti-apoptotic effects which may be partially mediated through a novel pathway that alters the concentration of mitochondrially-associated miRNAs.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Health Science and Medicine, Bond University, Gold Coast, Queensland 4227, Australia. james.keane@alumni.bond.edu.au.

ABSTRACT
The purpose of this study was to determine whether recombinant human growth hormone (rhGH) would show any significant effects on the expression of apoptosis regulating proteins in peripheral blood mononuclear cells (PBMCs). Additionally, the potential for post-transcriptional regulation of gene expression by miRNA was assessed in two cellular compartments, the cytosol and the mitochondria. Ten male subjects were subcutaneously injected with either rhGH (1 mg) or saline (0.9%) for seven consecutive days in a double-blinded fashion. Blood sampling was undertaken prior to treatment administration and over a period of three weeks following treatment cessation. Bcl-2 and Bak gene and protein expression levels were measured in PBMCs, while attention was also directed to the expression of miR-181a and miR-125b, known translational inhibitors of Bcl-2 and Bak respectively. Results showed that rhGH significantly decreased Bak protein concentrations compared to placebo samples for up to 8 days post treatment. While cytosolic miRNA expression was not found to be significantly affected by rhGH, measurement of the expression of miR-125b in mitochondrial fractions showed a significant down-regulation eight days post-rhGH administration. These findings suggest that rhGH induces short-term anti-apoptotic effects which may be partially mediated through a novel pathway that alters the concentration of mitochondrially-associated miRNAs.

No MeSH data available.