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The Stable Level of Glutamine synthetase 2 Plays an Important Role in Rice Growth and in Carbon-Nitrogen Metabolic Balance.

Bao A, Zhao Z, Ding G, Shi L, Xu F, Cai H - Int J Mol Sci (2015)

Bottom Line: The results revealed that the GS2-cosuppressed plants exhibited a poor plant growth phenotype and a poor nitrogen transport ability, which led to nitrogen accumulation and a decline in the carbon/nitrogen ratio in the stems.Interestingly, there was a higher concentration of soluble proteins and a lower concentration of carbohydrates in the GS2-cosuppressed plants at the seedling stage, while a contrasting result was displayed at the tillering stage.These results indicated the important role of a stable level of GS2 transcription during normal rice development and the importance of the carbon-nitrogen metabolic balance in rice growth.

View Article: PubMed Central - PubMed

Affiliation: Microelement Research Center, Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. baoaili19870212@163.com.

ABSTRACT
Glutamine synthetase 2 (GS2) is a key enzyme involved in the ammonium metabolism in plant leaves. In our previous study, we obtained GS2-cosuppressed plants, which displayed a normal growth phenotype at the seedling stage, while at the tillering stage they showed a chlorosis phenotype. In this study, to investigate the chlorosis mechanism, we systematically analyzed the plant growth, carbon-nitrogen metabolism and gene expressions between the GS2-cosuppressed rice and wild-type plants. The results revealed that the GS2-cosuppressed plants exhibited a poor plant growth phenotype and a poor nitrogen transport ability, which led to nitrogen accumulation and a decline in the carbon/nitrogen ratio in the stems. Interestingly, there was a higher concentration of soluble proteins and a lower concentration of carbohydrates in the GS2-cosuppressed plants at the seedling stage, while a contrasting result was displayed at the tillering stage. The analysis of the metabolic profile showed a significant increase of sugars and organic acids. Additionally, gene expression patterns were different in root and leaf of GS2-cosuppressed plants between the seedling and tillering stage. These results indicated the important role of a stable level of GS2 transcription during normal rice development and the importance of the carbon-nitrogen metabolic balance in rice growth.

No MeSH data available.


Related in: MedlinePlus

Summary of the differences between the seedling stage and the tillering stage in the GS2 transgenic plant. The symbols ↑ and ⊺ indicate induction and repression, respectively.
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ijms-16-12713-f007: Summary of the differences between the seedling stage and the tillering stage in the GS2 transgenic plant. The symbols ↑ and ⊺ indicate induction and repression, respectively.

Mentions: In this study, we systematically analyzed the differences in the growth phenotype, carbon-nitrogen metabolic status and gene expression profile between GS2-cosuppressed rice and wild-type Zhonghua 11 at both the seedling stage and the tillering stage under three different nitrogen conditions (N, G and N + G). Results demonstrated the close relationships between the GS2 mRNA levels and carbon-nitrogen metabolic status, as well as the plant growth phenotype. Figure 7 summarizes the differences between the seedling stage and the tillering stage in the GS2 transgenic plants.


The Stable Level of Glutamine synthetase 2 Plays an Important Role in Rice Growth and in Carbon-Nitrogen Metabolic Balance.

Bao A, Zhao Z, Ding G, Shi L, Xu F, Cai H - Int J Mol Sci (2015)

Summary of the differences between the seedling stage and the tillering stage in the GS2 transgenic plant. The symbols ↑ and ⊺ indicate induction and repression, respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490469&req=5

ijms-16-12713-f007: Summary of the differences between the seedling stage and the tillering stage in the GS2 transgenic plant. The symbols ↑ and ⊺ indicate induction and repression, respectively.
Mentions: In this study, we systematically analyzed the differences in the growth phenotype, carbon-nitrogen metabolic status and gene expression profile between GS2-cosuppressed rice and wild-type Zhonghua 11 at both the seedling stage and the tillering stage under three different nitrogen conditions (N, G and N + G). Results demonstrated the close relationships between the GS2 mRNA levels and carbon-nitrogen metabolic status, as well as the plant growth phenotype. Figure 7 summarizes the differences between the seedling stage and the tillering stage in the GS2 transgenic plants.

Bottom Line: The results revealed that the GS2-cosuppressed plants exhibited a poor plant growth phenotype and a poor nitrogen transport ability, which led to nitrogen accumulation and a decline in the carbon/nitrogen ratio in the stems.Interestingly, there was a higher concentration of soluble proteins and a lower concentration of carbohydrates in the GS2-cosuppressed plants at the seedling stage, while a contrasting result was displayed at the tillering stage.These results indicated the important role of a stable level of GS2 transcription during normal rice development and the importance of the carbon-nitrogen metabolic balance in rice growth.

View Article: PubMed Central - PubMed

Affiliation: Microelement Research Center, Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China. baoaili19870212@163.com.

ABSTRACT
Glutamine synthetase 2 (GS2) is a key enzyme involved in the ammonium metabolism in plant leaves. In our previous study, we obtained GS2-cosuppressed plants, which displayed a normal growth phenotype at the seedling stage, while at the tillering stage they showed a chlorosis phenotype. In this study, to investigate the chlorosis mechanism, we systematically analyzed the plant growth, carbon-nitrogen metabolism and gene expressions between the GS2-cosuppressed rice and wild-type plants. The results revealed that the GS2-cosuppressed plants exhibited a poor plant growth phenotype and a poor nitrogen transport ability, which led to nitrogen accumulation and a decline in the carbon/nitrogen ratio in the stems. Interestingly, there was a higher concentration of soluble proteins and a lower concentration of carbohydrates in the GS2-cosuppressed plants at the seedling stage, while a contrasting result was displayed at the tillering stage. The analysis of the metabolic profile showed a significant increase of sugars and organic acids. Additionally, gene expression patterns were different in root and leaf of GS2-cosuppressed plants between the seedling and tillering stage. These results indicated the important role of a stable level of GS2 transcription during normal rice development and the importance of the carbon-nitrogen metabolic balance in rice growth.

No MeSH data available.


Related in: MedlinePlus