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Single Cell Electrical Characterization Techniques.

Mansor MA, Ahmad MR - Int J Mol Sci (2015)

Bottom Line: This growing interest was supported by the emergence of various microfluidic techniques to fulfill high precisions screening, reduced equipment cost and low analysis time for characterization of the single cell's electrical properties, as compared to classical bulky technique.This paper presents a historical review of single cell electrical properties analysis development from classical techniques to recent advances in microfluidic techniques.Technical details of the different microfluidic techniques are highlighted, and the advantages and limitations of various microfluidic devices are discussed.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310-UTM Skudai, Johor, Malaysia. asraf@biomedical.utm.my.

ABSTRACT
Electrical properties of living cells have been proven to play significant roles in understanding of various biological activities including disease progression both at the cellular and molecular levels. Since two decades ago, many researchers have developed tools to analyze the cell's electrical states especially in single cell analysis (SCA). In depth analysis and more fully described activities of cell differentiation and cancer can only be accomplished with single cell analysis. This growing interest was supported by the emergence of various microfluidic techniques to fulfill high precisions screening, reduced equipment cost and low analysis time for characterization of the single cell's electrical properties, as compared to classical bulky technique. This paper presents a historical review of single cell electrical properties analysis development from classical techniques to recent advances in microfluidic techniques. Technical details of the different microfluidic techniques are highlighted, and the advantages and limitations of various microfluidic devices are discussed.

No MeSH data available.


Related in: MedlinePlus

(a) Illustrated a micro electrical impedance spectroscopy system using multielectrode configurations within an analysis cavity. Reprinted with permission from [97]; (b) Shown 3D schematic of the µ-EIS device incorporated with micropillars structure for capture the single cells; (c) Schematic diagram of cell measurement using DEP cell trapping technique. Reprinted with permission from [104].
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ijms-16-12686-f005: (a) Illustrated a micro electrical impedance spectroscopy system using multielectrode configurations within an analysis cavity. Reprinted with permission from [97]; (b) Shown 3D schematic of the µ-EIS device incorporated with micropillars structure for capture the single cells; (c) Schematic diagram of cell measurement using DEP cell trapping technique. Reprinted with permission from [104].

Mentions: First development of micro electrical impedance spectroscopy (µ-EIS) was reported in 2006 [97]. They developed microfluidic device which utilized the negative pressure to capture the single cell into the analysis cavity (Figure 5a). This device was used to measure the electrical impedance of human breast cancer cell lines of different pathological stages (MCF-7, MDA-MB-231, and MDA-MB-435) [18]. However this device has a disadvantage to monitor the cell capturing process using a microscope because the contrast difference in the silicon nitride membrane composing the cell traps area and the surroundings. The same group, Cho et al. [4] developed an array of horizontal cell traps of an µ-EIS device to overcome the limitation of the previous device. Negative pressure was used to capture single cells and impedance measurement was performed to obtain the electrical impedance spectra of metastatic head and neck cancer (HNC) cell lines. This device also can minimize the leakage current due to the position of cells formed in direct contact between cells and electrodes.


Single Cell Electrical Characterization Techniques.

Mansor MA, Ahmad MR - Int J Mol Sci (2015)

(a) Illustrated a micro electrical impedance spectroscopy system using multielectrode configurations within an analysis cavity. Reprinted with permission from [97]; (b) Shown 3D schematic of the µ-EIS device incorporated with micropillars structure for capture the single cells; (c) Schematic diagram of cell measurement using DEP cell trapping technique. Reprinted with permission from [104].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490468&req=5

ijms-16-12686-f005: (a) Illustrated a micro electrical impedance spectroscopy system using multielectrode configurations within an analysis cavity. Reprinted with permission from [97]; (b) Shown 3D schematic of the µ-EIS device incorporated with micropillars structure for capture the single cells; (c) Schematic diagram of cell measurement using DEP cell trapping technique. Reprinted with permission from [104].
Mentions: First development of micro electrical impedance spectroscopy (µ-EIS) was reported in 2006 [97]. They developed microfluidic device which utilized the negative pressure to capture the single cell into the analysis cavity (Figure 5a). This device was used to measure the electrical impedance of human breast cancer cell lines of different pathological stages (MCF-7, MDA-MB-231, and MDA-MB-435) [18]. However this device has a disadvantage to monitor the cell capturing process using a microscope because the contrast difference in the silicon nitride membrane composing the cell traps area and the surroundings. The same group, Cho et al. [4] developed an array of horizontal cell traps of an µ-EIS device to overcome the limitation of the previous device. Negative pressure was used to capture single cells and impedance measurement was performed to obtain the electrical impedance spectra of metastatic head and neck cancer (HNC) cell lines. This device also can minimize the leakage current due to the position of cells formed in direct contact between cells and electrodes.

Bottom Line: This growing interest was supported by the emergence of various microfluidic techniques to fulfill high precisions screening, reduced equipment cost and low analysis time for characterization of the single cell's electrical properties, as compared to classical bulky technique.This paper presents a historical review of single cell electrical properties analysis development from classical techniques to recent advances in microfluidic techniques.Technical details of the different microfluidic techniques are highlighted, and the advantages and limitations of various microfluidic devices are discussed.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310-UTM Skudai, Johor, Malaysia. asraf@biomedical.utm.my.

ABSTRACT
Electrical properties of living cells have been proven to play significant roles in understanding of various biological activities including disease progression both at the cellular and molecular levels. Since two decades ago, many researchers have developed tools to analyze the cell's electrical states especially in single cell analysis (SCA). In depth analysis and more fully described activities of cell differentiation and cancer can only be accomplished with single cell analysis. This growing interest was supported by the emergence of various microfluidic techniques to fulfill high precisions screening, reduced equipment cost and low analysis time for characterization of the single cell's electrical properties, as compared to classical bulky technique. This paper presents a historical review of single cell electrical properties analysis development from classical techniques to recent advances in microfluidic techniques. Technical details of the different microfluidic techniques are highlighted, and the advantages and limitations of various microfluidic devices are discussed.

No MeSH data available.


Related in: MedlinePlus