Limits...
Single Cell Electrical Characterization Techniques.

Mansor MA, Ahmad MR - Int J Mol Sci (2015)

Bottom Line: This growing interest was supported by the emergence of various microfluidic techniques to fulfill high precisions screening, reduced equipment cost and low analysis time for characterization of the single cell's electrical properties, as compared to classical bulky technique.This paper presents a historical review of single cell electrical properties analysis development from classical techniques to recent advances in microfluidic techniques.Technical details of the different microfluidic techniques are highlighted, and the advantages and limitations of various microfluidic devices are discussed.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310-UTM Skudai, Johor, Malaysia. asraf@biomedical.utm.my.

ABSTRACT
Electrical properties of living cells have been proven to play significant roles in understanding of various biological activities including disease progression both at the cellular and molecular levels. Since two decades ago, many researchers have developed tools to analyze the cell's electrical states especially in single cell analysis (SCA). In depth analysis and more fully described activities of cell differentiation and cancer can only be accomplished with single cell analysis. This growing interest was supported by the emergence of various microfluidic techniques to fulfill high precisions screening, reduced equipment cost and low analysis time for characterization of the single cell's electrical properties, as compared to classical bulky technique. This paper presents a historical review of single cell electrical properties analysis development from classical techniques to recent advances in microfluidic techniques. Technical details of the different microfluidic techniques are highlighted, and the advantages and limitations of various microfluidic devices are discussed.

No MeSH data available.


Related in: MedlinePlus

Schematic diagram of the Coulter counter working principle. Reprinted with permission from [72].
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4490468&req=5

ijms-16-12686-f003: Schematic diagram of the Coulter counter working principle. Reprinted with permission from [72].

Mentions: Flow cytometry is a fundamental and powerful analytical tool in cell biology and cellular disease diagnosis for many years. Flow cytometry has an ability to address some problems in single cell analysis such as identifiying, counting and sorting cells [68,69]. Based on laser-induced fluorescence detection in flow cytometry for single-cell studies within cell populations of relatively large sizes [70], flow cytometry creates an ideal scenario to analyze single cell electrical properties from a cell population. Coulter [71] developed the first flow cytometry tool having capability to measure the electrical properties of single particles, which is known as the microfluidic Coulter counter. A Coulter counter measures the changing of DC resistance between two electrically isolated fluid-filled chambers when microparticles act as an insulating layer at DC pass through a small connecting orifice. Figure 3 illustrates the working principle of the Coulter counter, where two large electrodes are placed on connecting chamber. When a particles or biological cells flow through a sensing aperture which has current flow, it will displace the conductive fluid and alters the resistance. The current flow was decreased as a particle passes through and for this reason, individual cells can be counted and sized [72]. The Coulter counter is limited to counting cells and classifying cell types based on size due to challenging of selecting electrode design and channel geometry [73]. The Microfluidic Coulter counter is incapable of characterizing electrical properties of cell. In order to determine the electrical properties of cells, Sohn et al. [74] developed flow cytometry based on capacitance principle to measure the DNA content of fixed eukaryotic cells. Electrical properties of individual cells were referred to distinct peaks measured by a capacitance bridge at 1 kHz frequency.


Single Cell Electrical Characterization Techniques.

Mansor MA, Ahmad MR - Int J Mol Sci (2015)

Schematic diagram of the Coulter counter working principle. Reprinted with permission from [72].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490468&req=5

ijms-16-12686-f003: Schematic diagram of the Coulter counter working principle. Reprinted with permission from [72].
Mentions: Flow cytometry is a fundamental and powerful analytical tool in cell biology and cellular disease diagnosis for many years. Flow cytometry has an ability to address some problems in single cell analysis such as identifiying, counting and sorting cells [68,69]. Based on laser-induced fluorescence detection in flow cytometry for single-cell studies within cell populations of relatively large sizes [70], flow cytometry creates an ideal scenario to analyze single cell electrical properties from a cell population. Coulter [71] developed the first flow cytometry tool having capability to measure the electrical properties of single particles, which is known as the microfluidic Coulter counter. A Coulter counter measures the changing of DC resistance between two electrically isolated fluid-filled chambers when microparticles act as an insulating layer at DC pass through a small connecting orifice. Figure 3 illustrates the working principle of the Coulter counter, where two large electrodes are placed on connecting chamber. When a particles or biological cells flow through a sensing aperture which has current flow, it will displace the conductive fluid and alters the resistance. The current flow was decreased as a particle passes through and for this reason, individual cells can be counted and sized [72]. The Coulter counter is limited to counting cells and classifying cell types based on size due to challenging of selecting electrode design and channel geometry [73]. The Microfluidic Coulter counter is incapable of characterizing electrical properties of cell. In order to determine the electrical properties of cells, Sohn et al. [74] developed flow cytometry based on capacitance principle to measure the DNA content of fixed eukaryotic cells. Electrical properties of individual cells were referred to distinct peaks measured by a capacitance bridge at 1 kHz frequency.

Bottom Line: This growing interest was supported by the emergence of various microfluidic techniques to fulfill high precisions screening, reduced equipment cost and low analysis time for characterization of the single cell's electrical properties, as compared to classical bulky technique.This paper presents a historical review of single cell electrical properties analysis development from classical techniques to recent advances in microfluidic techniques.Technical details of the different microfluidic techniques are highlighted, and the advantages and limitations of various microfluidic devices are discussed.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310-UTM Skudai, Johor, Malaysia. asraf@biomedical.utm.my.

ABSTRACT
Electrical properties of living cells have been proven to play significant roles in understanding of various biological activities including disease progression both at the cellular and molecular levels. Since two decades ago, many researchers have developed tools to analyze the cell's electrical states especially in single cell analysis (SCA). In depth analysis and more fully described activities of cell differentiation and cancer can only be accomplished with single cell analysis. This growing interest was supported by the emergence of various microfluidic techniques to fulfill high precisions screening, reduced equipment cost and low analysis time for characterization of the single cell's electrical properties, as compared to classical bulky technique. This paper presents a historical review of single cell electrical properties analysis development from classical techniques to recent advances in microfluidic techniques. Technical details of the different microfluidic techniques are highlighted, and the advantages and limitations of various microfluidic devices are discussed.

No MeSH data available.


Related in: MedlinePlus