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Single Cell Electrical Characterization Techniques.

Mansor MA, Ahmad MR - Int J Mol Sci (2015)

Bottom Line: This growing interest was supported by the emergence of various microfluidic techniques to fulfill high precisions screening, reduced equipment cost and low analysis time for characterization of the single cell's electrical properties, as compared to classical bulky technique.This paper presents a historical review of single cell electrical properties analysis development from classical techniques to recent advances in microfluidic techniques.Technical details of the different microfluidic techniques are highlighted, and the advantages and limitations of various microfluidic devices are discussed.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310-UTM Skudai, Johor, Malaysia. asraf@biomedical.utm.my.

ABSTRACT
Electrical properties of living cells have been proven to play significant roles in understanding of various biological activities including disease progression both at the cellular and molecular levels. Since two decades ago, many researchers have developed tools to analyze the cell's electrical states especially in single cell analysis (SCA). In depth analysis and more fully described activities of cell differentiation and cancer can only be accomplished with single cell analysis. This growing interest was supported by the emergence of various microfluidic techniques to fulfill high precisions screening, reduced equipment cost and low analysis time for characterization of the single cell's electrical properties, as compared to classical bulky technique. This paper presents a historical review of single cell electrical properties analysis development from classical techniques to recent advances in microfluidic techniques. Technical details of the different microfluidic techniques are highlighted, and the advantages and limitations of various microfluidic devices are discussed.

No MeSH data available.


Related in: MedlinePlus

(a) An illustration of the working principle of electrorotation to analyse single cells; (b) The electrorotation (ROT)-microchip incorporated with the 3D octode. nQDEP (negative quadrupole dielectrophoresis) signal, Asin (ω1t + 0°) and Asin (ω1t + 180°) are used for a single cell trapping, while the ROT signals, Bsin(ω2t + 0°), Bsin (ω2t + 90°), Bsin (ω2t + 180°) and Bsin (ω2t + 270°) are used to simultaneously generate torque. Reprinted with permission from [56].
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ijms-16-12686-f002: (a) An illustration of the working principle of electrorotation to analyse single cells; (b) The electrorotation (ROT)-microchip incorporated with the 3D octode. nQDEP (negative quadrupole dielectrophoresis) signal, Asin (ω1t + 0°) and Asin (ω1t + 180°) are used for a single cell trapping, while the ROT signals, Bsin(ω2t + 0°), Bsin (ω2t + 90°), Bsin (ω2t + 180°) and Bsin (ω2t + 270°) are used to simultaneously generate torque. Reprinted with permission from [56].

Mentions: The quadrupole electrodes connected to sine wave was a famous design in ROT technique [48,49,50]. Figure 2a shows a working principle of ROT, four electrodes were energized by sinusoidal signal generator created rotating electrical field, E. Laser tweezers were used to drag a single cell to the center of a four-electrode chamber, then a single cell, P will rotate in either the same direction (co-field) or in the opposite direction (anti-field) to the rotating field [51]. The direction was taken by the cell depending to the dielectric properties of the cell and suspending medium along with the frequency of the electric field. The dielectric properties of a single cell can then be extracted by utilizing Maxwell’s mixture theory, to associate the complex permittivity of the suspension to the complex permittivity of the cell [49]. More detail on theory and working principle of electrorotation can be found in other articles [52,53,54] and a book [55].


Single Cell Electrical Characterization Techniques.

Mansor MA, Ahmad MR - Int J Mol Sci (2015)

(a) An illustration of the working principle of electrorotation to analyse single cells; (b) The electrorotation (ROT)-microchip incorporated with the 3D octode. nQDEP (negative quadrupole dielectrophoresis) signal, Asin (ω1t + 0°) and Asin (ω1t + 180°) are used for a single cell trapping, while the ROT signals, Bsin(ω2t + 0°), Bsin (ω2t + 90°), Bsin (ω2t + 180°) and Bsin (ω2t + 270°) are used to simultaneously generate torque. Reprinted with permission from [56].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490468&req=5

ijms-16-12686-f002: (a) An illustration of the working principle of electrorotation to analyse single cells; (b) The electrorotation (ROT)-microchip incorporated with the 3D octode. nQDEP (negative quadrupole dielectrophoresis) signal, Asin (ω1t + 0°) and Asin (ω1t + 180°) are used for a single cell trapping, while the ROT signals, Bsin(ω2t + 0°), Bsin (ω2t + 90°), Bsin (ω2t + 180°) and Bsin (ω2t + 270°) are used to simultaneously generate torque. Reprinted with permission from [56].
Mentions: The quadrupole electrodes connected to sine wave was a famous design in ROT technique [48,49,50]. Figure 2a shows a working principle of ROT, four electrodes were energized by sinusoidal signal generator created rotating electrical field, E. Laser tweezers were used to drag a single cell to the center of a four-electrode chamber, then a single cell, P will rotate in either the same direction (co-field) or in the opposite direction (anti-field) to the rotating field [51]. The direction was taken by the cell depending to the dielectric properties of the cell and suspending medium along with the frequency of the electric field. The dielectric properties of a single cell can then be extracted by utilizing Maxwell’s mixture theory, to associate the complex permittivity of the suspension to the complex permittivity of the cell [49]. More detail on theory and working principle of electrorotation can be found in other articles [52,53,54] and a book [55].

Bottom Line: This growing interest was supported by the emergence of various microfluidic techniques to fulfill high precisions screening, reduced equipment cost and low analysis time for characterization of the single cell's electrical properties, as compared to classical bulky technique.This paper presents a historical review of single cell electrical properties analysis development from classical techniques to recent advances in microfluidic techniques.Technical details of the different microfluidic techniques are highlighted, and the advantages and limitations of various microfluidic devices are discussed.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, 81310-UTM Skudai, Johor, Malaysia. asraf@biomedical.utm.my.

ABSTRACT
Electrical properties of living cells have been proven to play significant roles in understanding of various biological activities including disease progression both at the cellular and molecular levels. Since two decades ago, many researchers have developed tools to analyze the cell's electrical states especially in single cell analysis (SCA). In depth analysis and more fully described activities of cell differentiation and cancer can only be accomplished with single cell analysis. This growing interest was supported by the emergence of various microfluidic techniques to fulfill high precisions screening, reduced equipment cost and low analysis time for characterization of the single cell's electrical properties, as compared to classical bulky technique. This paper presents a historical review of single cell electrical properties analysis development from classical techniques to recent advances in microfluidic techniques. Technical details of the different microfluidic techniques are highlighted, and the advantages and limitations of various microfluidic devices are discussed.

No MeSH data available.


Related in: MedlinePlus