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Specific Colon Cancer Cell Cytotoxicity Induced by Bacteriophage E Gene Expression under Transcriptional Control of Carcinoembryonic Antigen Promoter.

Rama AR, Hernandez R, Perazzoli G, Burgos M, Melguizo C, Vélez C, Prados J - Int J Mol Sci (2015)

Bottom Line: The bacteriophage E gene has demonstrated significant antitumor activity in several cancers, but without any tumor-specific activity.We used the carcinoembryonic antigen promoter (CEA) to direct E gene expression (pCEA-E) towards colon cancer cells. pCEA-E induced a high cell growth inhibition of human HTC-116 colon adenocarcinoma and mouse MC-38 colon cancer cells in comparison to normal human CCD18co colon cells, which have practically undetectable levels of CEA.These results suggest that the CEA promoter is an excellent candidate for directing E gene expression specifically toward colon cancer cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Health Science, University of Jaén, Jaén 23071, Spain. arama@ujaen.es.

ABSTRACT
Colorectal cancer is one of the most prevalent cancers in the world. Patients in advanced stages often develop metastases that require chemotherapy and usually show a poor response, have a low survival rate and develop considerable toxicity with adverse symptoms. Gene therapy may act as an adjuvant therapy in attempts to destroy the tumor without affecting normal host tissue. The bacteriophage E gene has demonstrated significant antitumor activity in several cancers, but without any tumor-specific activity. The use of tumor-specific promoters may help to direct the expression of therapeutic genes so they act against specific cancer cells. We used the carcinoembryonic antigen promoter (CEA) to direct E gene expression (pCEA-E) towards colon cancer cells. pCEA-E induced a high cell growth inhibition of human HTC-116 colon adenocarcinoma and mouse MC-38 colon cancer cells in comparison to normal human CCD18co colon cells, which have practically undetectable levels of CEA. In addition, in vivo analyses of mice bearing tumors induced using MC-38 cells showed a significant decrease in tumor volume after pCEA-E treatment and a low level of Ki-67 in relation to untreated tumors. These results suggest that the CEA promoter is an excellent candidate for directing E gene expression specifically toward colon cancer cells.

No MeSH data available.


Related in: MedlinePlus

Tumor growth inhibition induced by pCEA-E. Treatment with pCEA-E induced a significant reduction in tumor volume at the end of the study period (33 days) in comparison with the growth of pCEA-LacZ-treated or untreated tumors (control) (p < 0.05). Data represent the mean value ± SEM.
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ijms-16-12601-f004: Tumor growth inhibition induced by pCEA-E. Treatment with pCEA-E induced a significant reduction in tumor volume at the end of the study period (33 days) in comparison with the growth of pCEA-LacZ-treated or untreated tumors (control) (p < 0.05). Data represent the mean value ± SEM.

Mentions: As Figure 4 shows, intratumoral treatment with pCEA-E produced significant reductions in tumor volumes after 33 days. Specifically, pCEA-E was able to induce a 36% tumor volume reduction in comparison to the control (p < 0.05). pCEA-LacZ treatment, on the other hand, did not bring about any modifications in tumor growth rates, yielding similar results to those observed in the untreated mouse group. Nevertheless, although pCEA-E treatment produced reductions in tumor volumes, it failed to increase mouse survival rates compared to the untreated control group (data not shown).


Specific Colon Cancer Cell Cytotoxicity Induced by Bacteriophage E Gene Expression under Transcriptional Control of Carcinoembryonic Antigen Promoter.

Rama AR, Hernandez R, Perazzoli G, Burgos M, Melguizo C, Vélez C, Prados J - Int J Mol Sci (2015)

Tumor growth inhibition induced by pCEA-E. Treatment with pCEA-E induced a significant reduction in tumor volume at the end of the study period (33 days) in comparison with the growth of pCEA-LacZ-treated or untreated tumors (control) (p < 0.05). Data represent the mean value ± SEM.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490463&req=5

ijms-16-12601-f004: Tumor growth inhibition induced by pCEA-E. Treatment with pCEA-E induced a significant reduction in tumor volume at the end of the study period (33 days) in comparison with the growth of pCEA-LacZ-treated or untreated tumors (control) (p < 0.05). Data represent the mean value ± SEM.
Mentions: As Figure 4 shows, intratumoral treatment with pCEA-E produced significant reductions in tumor volumes after 33 days. Specifically, pCEA-E was able to induce a 36% tumor volume reduction in comparison to the control (p < 0.05). pCEA-LacZ treatment, on the other hand, did not bring about any modifications in tumor growth rates, yielding similar results to those observed in the untreated mouse group. Nevertheless, although pCEA-E treatment produced reductions in tumor volumes, it failed to increase mouse survival rates compared to the untreated control group (data not shown).

Bottom Line: The bacteriophage E gene has demonstrated significant antitumor activity in several cancers, but without any tumor-specific activity.We used the carcinoembryonic antigen promoter (CEA) to direct E gene expression (pCEA-E) towards colon cancer cells. pCEA-E induced a high cell growth inhibition of human HTC-116 colon adenocarcinoma and mouse MC-38 colon cancer cells in comparison to normal human CCD18co colon cells, which have practically undetectable levels of CEA.These results suggest that the CEA promoter is an excellent candidate for directing E gene expression specifically toward colon cancer cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Health Science, University of Jaén, Jaén 23071, Spain. arama@ujaen.es.

ABSTRACT
Colorectal cancer is one of the most prevalent cancers in the world. Patients in advanced stages often develop metastases that require chemotherapy and usually show a poor response, have a low survival rate and develop considerable toxicity with adverse symptoms. Gene therapy may act as an adjuvant therapy in attempts to destroy the tumor without affecting normal host tissue. The bacteriophage E gene has demonstrated significant antitumor activity in several cancers, but without any tumor-specific activity. The use of tumor-specific promoters may help to direct the expression of therapeutic genes so they act against specific cancer cells. We used the carcinoembryonic antigen promoter (CEA) to direct E gene expression (pCEA-E) towards colon cancer cells. pCEA-E induced a high cell growth inhibition of human HTC-116 colon adenocarcinoma and mouse MC-38 colon cancer cells in comparison to normal human CCD18co colon cells, which have practically undetectable levels of CEA. In addition, in vivo analyses of mice bearing tumors induced using MC-38 cells showed a significant decrease in tumor volume after pCEA-E treatment and a low level of Ki-67 in relation to untreated tumors. These results suggest that the CEA promoter is an excellent candidate for directing E gene expression specifically toward colon cancer cells.

No MeSH data available.


Related in: MedlinePlus