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Production of Galactooligosaccharides Using β-Galactosidase Immobilized on Chitosan-Coated Magnetic Nanoparticles with Tris(hydroxymethyl)phosphine as an Optional Coupling Agent.

Chen SC, Duan KJ - Int J Mol Sci (2015)

Bottom Line: However, activity retention of batchwise reactions was similar for both immobilized systems.All the three enzyme systems produced GOS compound with similar concentration profiles, with a maximum GOS yield of 50.5% from 36% (w · v(-1)) lactose on a dry weight basis.The chitosan-coated magnetic Fe3O4 nanoparticles can be regenerated using a desorption/re-adsorption process described in this study.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, Tatung University, Taipei 104, Taiwan. monica94707@yahoo.com.tw.

ABSTRACT
β-Galactosidase was immobilized on chitosan-coated magnetic Fe3O4 nanoparticles and was used to produce galactooligosaccharides (GOS) from lactose. Immobilized enzyme was prepared with or without the coupling agent, tris(hydroxymethyl)phosphine (THP). The two immobilized systems and the free enzyme achieved their maximum activity at pH 6.0 with an optimal temperature of 50 °C. The immobilized enzymes showed higher activities at a wider range of temperatures and pH. Furthermore, the immobilized enzyme coupled with THP showed higher thermal stability than that without THP. However, activity retention of batchwise reactions was similar for both immobilized systems. All the three enzyme systems produced GOS compound with similar concentration profiles, with a maximum GOS yield of 50.5% from 36% (w · v(-1)) lactose on a dry weight basis. The chitosan-coated magnetic Fe3O4 nanoparticles can be regenerated using a desorption/re-adsorption process described in this study.

No MeSH data available.


Total GOS production for the three enzyme systems. (Δ) total GOS of free enzyme; (▽) total GOS of Fe3O4-CS-immobilized enzyme; (☆) total GOS of Fe3O4-CS-THP-immobilized enzyme.
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ijms-16-12499-f008: Total GOS production for the three enzyme systems. (Δ) total GOS of free enzyme; (▽) total GOS of Fe3O4-CS-immobilized enzyme; (☆) total GOS of Fe3O4-CS-THP-immobilized enzyme.

Mentions: When the amounts of di-, tri-, and tetrasaccharides were combined to calculate total GOS produced, the results showed that the immobilized and free enzymes all had a maximum GOS yield of 50.5% on a dry weight basis from 36% lactose (w/v) (Figure 8). It should be noted that only a 41% yield of GOS was obtained when β-galactosidase was immobilized on porous chitosan beads, because diffusion resistance generated reduced lactose concentrations on the surface of the beads [5]. Under a lower lactose concentration, galactoside is more prone to bind with H2O and therefore results a lower yield of GOS from lactose. The nanoparticles take advantage of their high surface area to immobilize sufficient amount of enzyme. Furthermore, the mass transfer resistance of lactose from bulk solution to surface of the nanoparticles is relatively low. It is not surprising that the immobilized enzyme systems performed almost the same reaction characteristics as the free enzyme system.


Production of Galactooligosaccharides Using β-Galactosidase Immobilized on Chitosan-Coated Magnetic Nanoparticles with Tris(hydroxymethyl)phosphine as an Optional Coupling Agent.

Chen SC, Duan KJ - Int J Mol Sci (2015)

Total GOS production for the three enzyme systems. (Δ) total GOS of free enzyme; (▽) total GOS of Fe3O4-CS-immobilized enzyme; (☆) total GOS of Fe3O4-CS-THP-immobilized enzyme.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490457&req=5

ijms-16-12499-f008: Total GOS production for the three enzyme systems. (Δ) total GOS of free enzyme; (▽) total GOS of Fe3O4-CS-immobilized enzyme; (☆) total GOS of Fe3O4-CS-THP-immobilized enzyme.
Mentions: When the amounts of di-, tri-, and tetrasaccharides were combined to calculate total GOS produced, the results showed that the immobilized and free enzymes all had a maximum GOS yield of 50.5% on a dry weight basis from 36% lactose (w/v) (Figure 8). It should be noted that only a 41% yield of GOS was obtained when β-galactosidase was immobilized on porous chitosan beads, because diffusion resistance generated reduced lactose concentrations on the surface of the beads [5]. Under a lower lactose concentration, galactoside is more prone to bind with H2O and therefore results a lower yield of GOS from lactose. The nanoparticles take advantage of their high surface area to immobilize sufficient amount of enzyme. Furthermore, the mass transfer resistance of lactose from bulk solution to surface of the nanoparticles is relatively low. It is not surprising that the immobilized enzyme systems performed almost the same reaction characteristics as the free enzyme system.

Bottom Line: However, activity retention of batchwise reactions was similar for both immobilized systems.All the three enzyme systems produced GOS compound with similar concentration profiles, with a maximum GOS yield of 50.5% from 36% (w · v(-1)) lactose on a dry weight basis.The chitosan-coated magnetic Fe3O4 nanoparticles can be regenerated using a desorption/re-adsorption process described in this study.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioengineering, Tatung University, Taipei 104, Taiwan. monica94707@yahoo.com.tw.

ABSTRACT
β-Galactosidase was immobilized on chitosan-coated magnetic Fe3O4 nanoparticles and was used to produce galactooligosaccharides (GOS) from lactose. Immobilized enzyme was prepared with or without the coupling agent, tris(hydroxymethyl)phosphine (THP). The two immobilized systems and the free enzyme achieved their maximum activity at pH 6.0 with an optimal temperature of 50 °C. The immobilized enzymes showed higher activities at a wider range of temperatures and pH. Furthermore, the immobilized enzyme coupled with THP showed higher thermal stability than that without THP. However, activity retention of batchwise reactions was similar for both immobilized systems. All the three enzyme systems produced GOS compound with similar concentration profiles, with a maximum GOS yield of 50.5% from 36% (w · v(-1)) lactose on a dry weight basis. The chitosan-coated magnetic Fe3O4 nanoparticles can be regenerated using a desorption/re-adsorption process described in this study.

No MeSH data available.