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The Anti-Inflammatory Effects of the Methanolic Extract and Fractions from Davilla elliptica St. Hil. (Dilleniaceae) on Bothrops jararaca Envenomation.

Nishijima CM, Delella FK, Rodrigues CM, Rinaldo D, Lopes-Ferreira MV, da Rocha LR, Vilegas W, Felisbino SL, Hiruma-Lima CA - Int J Mol Sci (2015)

Bottom Line: Therefore, this work was undertaken to investigate the anti-inflammatory effects of the methanolic extract and fractions from D. elliptica and to evaluate the role of matrix metalloproteinases (MMPs) in this process.Our results show that intraperitoneal (i.p.) injection of fractions significantly reduced paw oedema after the carrageenan challenge.These results confirmed the anti-inflammatory activity of the methanolic extract and tannins fraction of D. elliptica and showed that the dermonecrosis properties of B. jararaca venom might be mediated through the inhibition of MMP-9 activity.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiologia, Instituto de Biociências, Universidade Estadual Paulista-UNESP, CEP 18618-970 Botucatu, São Paulo, Brazil. cat.nishi@ig.com.br.

ABSTRACT
Inflammation and haemorrhage are the main characteristics of tissue injury in botropic envenomation. Although some studies have shown that anti-venom prevents systemic reactions, it is not efficient in preventing tissue injury at the site of the bite. Therefore, this work was undertaken to investigate the anti-inflammatory effects of the methanolic extract and fractions from D. elliptica and to evaluate the role of matrix metalloproteinases (MMPs) in this process. Effects of the extract and fractions from D. elliptica were evaluated using a carrageenan-induced paw oedema model in rats, and leukocyte rolling was visualized by intravital. The quantification of MMPs activities (MMP-2 and MMP-9) extracted from the dermis of mice treated with extract and fractions alone or incubated with venom was determined by zymographic analyses. Our results show that intraperitoneal (i.p.) injection of fractions significantly reduced paw oedema after the carrageenan challenge. Treatment with the tannins fraction also resulted in considerable inhibition of the rolling of leukocytes and this fraction was able to decrease the activation of MMP-9. These results confirmed the anti-inflammatory activity of the methanolic extract and tannins fraction of D. elliptica and showed that the dermonecrosis properties of B. jararaca venom might be mediated through the inhibition of MMP-9 activity.

No MeSH data available.


Related in: MedlinePlus

Effect of the methanolic extract from Davilla elliptica on the gelatinolytic activity of MMP-9 and MMP-2 in mouse dermis. (A) Gelatinolytic activity of MMP-2 and MMP-9: PBS (lane 1); Methanolic extract (E) (lane 2); venom (V) (lane 3); venom + methanolic extract (V + E) (lane 4); (B) The gelatinolytic activity of MMP-9 by integrated optical density (IOD); (C) The gelatinolytic activity of MMP-2 by integrated optical density (IOD). The results are presented as fold increases.
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ijms-16-12454-f002: Effect of the methanolic extract from Davilla elliptica on the gelatinolytic activity of MMP-9 and MMP-2 in mouse dermis. (A) Gelatinolytic activity of MMP-2 and MMP-9: PBS (lane 1); Methanolic extract (E) (lane 2); venom (V) (lane 3); venom + methanolic extract (V + E) (lane 4); (B) The gelatinolytic activity of MMP-9 by integrated optical density (IOD); (C) The gelatinolytic activity of MMP-2 by integrated optical density (IOD). The results are presented as fold increases.

Mentions: In this study, after three days of application of B. jararaca venom into the dermis, we observed through the zymography technique that the venom increased the activity of pro-MMP-2, active-MMP-2, pro-MMP-9 and active-MMP-9 and there was no change in the activity of intermediate MMP-2 in relation to the PBS group (Figure 2 and Figure 3). The extract and tannins fraction were able to decrease the activation of MMP-9 induced by the venom, but the same result was not observed with the flavonoids fraction from D. elliptica. The tannins fraction also decreased the activity of pro-MMP-2 in relation to the venom. Similar results were obtained in relation to paw oedema, as we found that only the extract and tannins fraction decreased the oedema induced by carrageenan.


The Anti-Inflammatory Effects of the Methanolic Extract and Fractions from Davilla elliptica St. Hil. (Dilleniaceae) on Bothrops jararaca Envenomation.

Nishijima CM, Delella FK, Rodrigues CM, Rinaldo D, Lopes-Ferreira MV, da Rocha LR, Vilegas W, Felisbino SL, Hiruma-Lima CA - Int J Mol Sci (2015)

Effect of the methanolic extract from Davilla elliptica on the gelatinolytic activity of MMP-9 and MMP-2 in mouse dermis. (A) Gelatinolytic activity of MMP-2 and MMP-9: PBS (lane 1); Methanolic extract (E) (lane 2); venom (V) (lane 3); venom + methanolic extract (V + E) (lane 4); (B) The gelatinolytic activity of MMP-9 by integrated optical density (IOD); (C) The gelatinolytic activity of MMP-2 by integrated optical density (IOD). The results are presented as fold increases.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490454&req=5

ijms-16-12454-f002: Effect of the methanolic extract from Davilla elliptica on the gelatinolytic activity of MMP-9 and MMP-2 in mouse dermis. (A) Gelatinolytic activity of MMP-2 and MMP-9: PBS (lane 1); Methanolic extract (E) (lane 2); venom (V) (lane 3); venom + methanolic extract (V + E) (lane 4); (B) The gelatinolytic activity of MMP-9 by integrated optical density (IOD); (C) The gelatinolytic activity of MMP-2 by integrated optical density (IOD). The results are presented as fold increases.
Mentions: In this study, after three days of application of B. jararaca venom into the dermis, we observed through the zymography technique that the venom increased the activity of pro-MMP-2, active-MMP-2, pro-MMP-9 and active-MMP-9 and there was no change in the activity of intermediate MMP-2 in relation to the PBS group (Figure 2 and Figure 3). The extract and tannins fraction were able to decrease the activation of MMP-9 induced by the venom, but the same result was not observed with the flavonoids fraction from D. elliptica. The tannins fraction also decreased the activity of pro-MMP-2 in relation to the venom. Similar results were obtained in relation to paw oedema, as we found that only the extract and tannins fraction decreased the oedema induced by carrageenan.

Bottom Line: Therefore, this work was undertaken to investigate the anti-inflammatory effects of the methanolic extract and fractions from D. elliptica and to evaluate the role of matrix metalloproteinases (MMPs) in this process.Our results show that intraperitoneal (i.p.) injection of fractions significantly reduced paw oedema after the carrageenan challenge.These results confirmed the anti-inflammatory activity of the methanolic extract and tannins fraction of D. elliptica and showed that the dermonecrosis properties of B. jararaca venom might be mediated through the inhibition of MMP-9 activity.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiologia, Instituto de Biociências, Universidade Estadual Paulista-UNESP, CEP 18618-970 Botucatu, São Paulo, Brazil. cat.nishi@ig.com.br.

ABSTRACT
Inflammation and haemorrhage are the main characteristics of tissue injury in botropic envenomation. Although some studies have shown that anti-venom prevents systemic reactions, it is not efficient in preventing tissue injury at the site of the bite. Therefore, this work was undertaken to investigate the anti-inflammatory effects of the methanolic extract and fractions from D. elliptica and to evaluate the role of matrix metalloproteinases (MMPs) in this process. Effects of the extract and fractions from D. elliptica were evaluated using a carrageenan-induced paw oedema model in rats, and leukocyte rolling was visualized by intravital. The quantification of MMPs activities (MMP-2 and MMP-9) extracted from the dermis of mice treated with extract and fractions alone or incubated with venom was determined by zymographic analyses. Our results show that intraperitoneal (i.p.) injection of fractions significantly reduced paw oedema after the carrageenan challenge. Treatment with the tannins fraction also resulted in considerable inhibition of the rolling of leukocytes and this fraction was able to decrease the activation of MMP-9. These results confirmed the anti-inflammatory activity of the methanolic extract and tannins fraction of D. elliptica and showed that the dermonecrosis properties of B. jararaca venom might be mediated through the inhibition of MMP-9 activity.

No MeSH data available.


Related in: MedlinePlus