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The Anti-Inflammatory Effects of the Methanolic Extract and Fractions from Davilla elliptica St. Hil. (Dilleniaceae) on Bothrops jararaca Envenomation.

Nishijima CM, Delella FK, Rodrigues CM, Rinaldo D, Lopes-Ferreira MV, da Rocha LR, Vilegas W, Felisbino SL, Hiruma-Lima CA - Int J Mol Sci (2015)

Bottom Line: Therefore, this work was undertaken to investigate the anti-inflammatory effects of the methanolic extract and fractions from D. elliptica and to evaluate the role of matrix metalloproteinases (MMPs) in this process.Our results show that intraperitoneal (i.p.) injection of fractions significantly reduced paw oedema after the carrageenan challenge.These results confirmed the anti-inflammatory activity of the methanolic extract and tannins fraction of D. elliptica and showed that the dermonecrosis properties of B. jararaca venom might be mediated through the inhibition of MMP-9 activity.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiologia, Instituto de Biociências, Universidade Estadual Paulista-UNESP, CEP 18618-970 Botucatu, São Paulo, Brazil. cat.nishi@ig.com.br.

ABSTRACT
Inflammation and haemorrhage are the main characteristics of tissue injury in botropic envenomation. Although some studies have shown that anti-venom prevents systemic reactions, it is not efficient in preventing tissue injury at the site of the bite. Therefore, this work was undertaken to investigate the anti-inflammatory effects of the methanolic extract and fractions from D. elliptica and to evaluate the role of matrix metalloproteinases (MMPs) in this process. Effects of the extract and fractions from D. elliptica were evaluated using a carrageenan-induced paw oedema model in rats, and leukocyte rolling was visualized by intravital. The quantification of MMPs activities (MMP-2 and MMP-9) extracted from the dermis of mice treated with extract and fractions alone or incubated with venom was determined by zymographic analyses. Our results show that intraperitoneal (i.p.) injection of fractions significantly reduced paw oedema after the carrageenan challenge. Treatment with the tannins fraction also resulted in considerable inhibition of the rolling of leukocytes and this fraction was able to decrease the activation of MMP-9. These results confirmed the anti-inflammatory activity of the methanolic extract and tannins fraction of D. elliptica and showed that the dermonecrosis properties of B. jararaca venom might be mediated through the inhibition of MMP-9 activity.

No MeSH data available.


Related in: MedlinePlus

Effect of the tannins’ fraction from D. elliptica on leukocyte rolling number after lipopolysaccharides (LPS) topical application to the cremaster muscle in mice (intravital microscopy).Data are means ± S.E.M (n = 11). ANOVA, followed by Dunnett’s test. ** p < 0.01 vs. the saline group within the same period of observation.
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ijms-16-12454-f001: Effect of the tannins’ fraction from D. elliptica on leukocyte rolling number after lipopolysaccharides (LPS) topical application to the cremaster muscle in mice (intravital microscopy).Data are means ± S.E.M (n = 11). ANOVA, followed by Dunnett’s test. ** p < 0.01 vs. the saline group within the same period of observation.

Mentions: Inflammation is the main characteristic of envenomation by the Bothrops genus, and after tissue injury, a variety of inflammatory mediators, such as vasoactive amines and eicosanoids are produced by endothelial cells and resident cells, especially mast cells, macrophages and dendritic cells near the injured site [19]. These mediators lead all of the inflammatory events in response to injury. Moreover, circulating leukocytes, initially neutrophils, migrate to the tissue under the influence of vasodilatation and chemotactic agents [20,21]. This process involves the rolling and adhesion of leukocytes in the microvascular endothelium, followed by transmigration through the vessel wall to the extravascular tissue. Leukocyte rolling was the important parameter for evaluating the anti-inflammatory effect of the tannins’ fraction of D. elliptica (15.62 mg/kg, i.p.). Using intravital microscopy, our results showed (Figure 1) that the tannins’ fraction caused a significant reduction in leukocyte rolling (50%) in the post-capillary venules of the cremaster muscle of mice induced by the topical application of lipopolysaccharides (LPS) (p < 0.01).


The Anti-Inflammatory Effects of the Methanolic Extract and Fractions from Davilla elliptica St. Hil. (Dilleniaceae) on Bothrops jararaca Envenomation.

Nishijima CM, Delella FK, Rodrigues CM, Rinaldo D, Lopes-Ferreira MV, da Rocha LR, Vilegas W, Felisbino SL, Hiruma-Lima CA - Int J Mol Sci (2015)

Effect of the tannins’ fraction from D. elliptica on leukocyte rolling number after lipopolysaccharides (LPS) topical application to the cremaster muscle in mice (intravital microscopy).Data are means ± S.E.M (n = 11). ANOVA, followed by Dunnett’s test. ** p < 0.01 vs. the saline group within the same period of observation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490454&req=5

ijms-16-12454-f001: Effect of the tannins’ fraction from D. elliptica on leukocyte rolling number after lipopolysaccharides (LPS) topical application to the cremaster muscle in mice (intravital microscopy).Data are means ± S.E.M (n = 11). ANOVA, followed by Dunnett’s test. ** p < 0.01 vs. the saline group within the same period of observation.
Mentions: Inflammation is the main characteristic of envenomation by the Bothrops genus, and after tissue injury, a variety of inflammatory mediators, such as vasoactive amines and eicosanoids are produced by endothelial cells and resident cells, especially mast cells, macrophages and dendritic cells near the injured site [19]. These mediators lead all of the inflammatory events in response to injury. Moreover, circulating leukocytes, initially neutrophils, migrate to the tissue under the influence of vasodilatation and chemotactic agents [20,21]. This process involves the rolling and adhesion of leukocytes in the microvascular endothelium, followed by transmigration through the vessel wall to the extravascular tissue. Leukocyte rolling was the important parameter for evaluating the anti-inflammatory effect of the tannins’ fraction of D. elliptica (15.62 mg/kg, i.p.). Using intravital microscopy, our results showed (Figure 1) that the tannins’ fraction caused a significant reduction in leukocyte rolling (50%) in the post-capillary venules of the cremaster muscle of mice induced by the topical application of lipopolysaccharides (LPS) (p < 0.01).

Bottom Line: Therefore, this work was undertaken to investigate the anti-inflammatory effects of the methanolic extract and fractions from D. elliptica and to evaluate the role of matrix metalloproteinases (MMPs) in this process.Our results show that intraperitoneal (i.p.) injection of fractions significantly reduced paw oedema after the carrageenan challenge.These results confirmed the anti-inflammatory activity of the methanolic extract and tannins fraction of D. elliptica and showed that the dermonecrosis properties of B. jararaca venom might be mediated through the inhibition of MMP-9 activity.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Fisiologia, Instituto de Biociências, Universidade Estadual Paulista-UNESP, CEP 18618-970 Botucatu, São Paulo, Brazil. cat.nishi@ig.com.br.

ABSTRACT
Inflammation and haemorrhage are the main characteristics of tissue injury in botropic envenomation. Although some studies have shown that anti-venom prevents systemic reactions, it is not efficient in preventing tissue injury at the site of the bite. Therefore, this work was undertaken to investigate the anti-inflammatory effects of the methanolic extract and fractions from D. elliptica and to evaluate the role of matrix metalloproteinases (MMPs) in this process. Effects of the extract and fractions from D. elliptica were evaluated using a carrageenan-induced paw oedema model in rats, and leukocyte rolling was visualized by intravital. The quantification of MMPs activities (MMP-2 and MMP-9) extracted from the dermis of mice treated with extract and fractions alone or incubated with venom was determined by zymographic analyses. Our results show that intraperitoneal (i.p.) injection of fractions significantly reduced paw oedema after the carrageenan challenge. Treatment with the tannins fraction also resulted in considerable inhibition of the rolling of leukocytes and this fraction was able to decrease the activation of MMP-9. These results confirmed the anti-inflammatory activity of the methanolic extract and tannins fraction of D. elliptica and showed that the dermonecrosis properties of B. jararaca venom might be mediated through the inhibition of MMP-9 activity.

No MeSH data available.


Related in: MedlinePlus