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Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia.

Souders CA, Maynard SE, Yan J, Wang Y, Boatright NK, Sedan J, Balyozian D, Cheslock PS, Molrine DC, Simas TA - Int J Mol Sci (2015)

Bottom Line: Here we describe the development and use of diagnostic monoclonal antibodies specific to the two main splice variants of sFlt1, sFlt1-1 and sFlt1-14.These antibodies were selected for their sensitivity and specificity to their respective sFlt1 isoform in a capture ELISA format.It may be possible to improve current diagnostic platforms if more specific antibodies are utilized.

View Article: PubMed Central - PubMed

Affiliation: MassBiologics of the University of Massachusetts Medical School, Boston, MA 02126, USA. Colby.Souders@UMassMed.edu.

ABSTRACT
Angiogenic biomarkers, including soluble fms-like tyrosine kinase 1 (sFlt1), are thought to be predictors of preeclampsia onset; however, improvement is needed before a widespread diagnostic test can be utilized. Here we describe the development and use of diagnostic monoclonal antibodies specific to the two main splice variants of sFlt1, sFlt1-1 and sFlt1-14. These antibodies were selected for their sensitivity and specificity to their respective sFlt1 isoform in a capture ELISA format. Data from this pilot study suggest that sFlt1-1 may be more predictive of preeclampsia than total sFlt1. It may be possible to improve current diagnostic platforms if more specific antibodies are utilized.

No MeSH data available.


Related in: MedlinePlus

Receiver operator curves generated from the sensitivity and specificity of sFlt1-1 and VEGFR-1 preeclampsia predictions at gestational windows 1 and 2 in (A) all samples measured and (B) a high-risk subset of these women with chronic hypertension and/or diabetes mellitus.
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ijms-16-12436-f006: Receiver operator curves generated from the sensitivity and specificity of sFlt1-1 and VEGFR-1 preeclampsia predictions at gestational windows 1 and 2 in (A) all samples measured and (B) a high-risk subset of these women with chronic hypertension and/or diabetes mellitus.

Mentions: These results suggest measurement of sFlt1 isoforms, particularly sFlt1-1, may be more predictive of preeclampsia as compared to VEGFR-1 (total sFlt1). Thus, receiver operator curves (ROC) were generated for subjects who had samples at both GW1 and GW2 time points (Figure 6). The area under the curve (AUC) for sFlt1-1 was greater as compared to VEGFR-1 for both GW1 and GW2 (Figure 6A) and, furthermore, the sFlt1-1 AUC at GW1 was comparable to that of VEGFR-1 at GW2. For subjects who developed preeclampsia, the GW1 sample was collected, on average, 10.2 weeks before preeclampsia diagnosis while collection at GW2 was a mean of 6.99 weeks prior to diagnosis, suggesting that sFlt1-1 may be as predictive as VEGFR-1 at least three weeks earlier. Similarly, the AUC is greater for sFlt1-1 compared to VEGFR-1 at both gestational windows for the subset of women with chronic hypertension and/or diabetes mellitus (Figure 6B).


Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia.

Souders CA, Maynard SE, Yan J, Wang Y, Boatright NK, Sedan J, Balyozian D, Cheslock PS, Molrine DC, Simas TA - Int J Mol Sci (2015)

Receiver operator curves generated from the sensitivity and specificity of sFlt1-1 and VEGFR-1 preeclampsia predictions at gestational windows 1 and 2 in (A) all samples measured and (B) a high-risk subset of these women with chronic hypertension and/or diabetes mellitus.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490453&req=5

ijms-16-12436-f006: Receiver operator curves generated from the sensitivity and specificity of sFlt1-1 and VEGFR-1 preeclampsia predictions at gestational windows 1 and 2 in (A) all samples measured and (B) a high-risk subset of these women with chronic hypertension and/or diabetes mellitus.
Mentions: These results suggest measurement of sFlt1 isoforms, particularly sFlt1-1, may be more predictive of preeclampsia as compared to VEGFR-1 (total sFlt1). Thus, receiver operator curves (ROC) were generated for subjects who had samples at both GW1 and GW2 time points (Figure 6). The area under the curve (AUC) for sFlt1-1 was greater as compared to VEGFR-1 for both GW1 and GW2 (Figure 6A) and, furthermore, the sFlt1-1 AUC at GW1 was comparable to that of VEGFR-1 at GW2. For subjects who developed preeclampsia, the GW1 sample was collected, on average, 10.2 weeks before preeclampsia diagnosis while collection at GW2 was a mean of 6.99 weeks prior to diagnosis, suggesting that sFlt1-1 may be as predictive as VEGFR-1 at least three weeks earlier. Similarly, the AUC is greater for sFlt1-1 compared to VEGFR-1 at both gestational windows for the subset of women with chronic hypertension and/or diabetes mellitus (Figure 6B).

Bottom Line: Here we describe the development and use of diagnostic monoclonal antibodies specific to the two main splice variants of sFlt1, sFlt1-1 and sFlt1-14.These antibodies were selected for their sensitivity and specificity to their respective sFlt1 isoform in a capture ELISA format.It may be possible to improve current diagnostic platforms if more specific antibodies are utilized.

View Article: PubMed Central - PubMed

Affiliation: MassBiologics of the University of Massachusetts Medical School, Boston, MA 02126, USA. Colby.Souders@UMassMed.edu.

ABSTRACT
Angiogenic biomarkers, including soluble fms-like tyrosine kinase 1 (sFlt1), are thought to be predictors of preeclampsia onset; however, improvement is needed before a widespread diagnostic test can be utilized. Here we describe the development and use of diagnostic monoclonal antibodies specific to the two main splice variants of sFlt1, sFlt1-1 and sFlt1-14. These antibodies were selected for their sensitivity and specificity to their respective sFlt1 isoform in a capture ELISA format. Data from this pilot study suggest that sFlt1-1 may be more predictive of preeclampsia than total sFlt1. It may be possible to improve current diagnostic platforms if more specific antibodies are utilized.

No MeSH data available.


Related in: MedlinePlus