Limits...
Induction of Apoptosis in Endometrial Cancer (Ishikawa) Cells by Pogostemon cablin Aqueous Extract (PCAE).

Tsai CC, Chang YH, Chang CC, Cheng YM, Ou YC, Chien CC, Hsu YC - Int J Mol Sci (2015)

Bottom Line: Gene expression profiling (GEP) results further suggest that, in addition to its known effects with regard to EC prevention, PCAE may also exert antitumor activity on established EC cells.Many previous studies have identified the chemo-preventive effects of natural plant materials and the potential role of these materials in chemotherapy.This current study used human EC Ishikawa cells to investigate the anti-tumor effects of PCAE in EC cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Kaohsiung 83301, Taiwan. nick@adm.cgmh.org.tw.

ABSTRACT
Pogostemon cablin (PC) is a traditional herbal medicine used in the treatment of the common cold, nausea, diarrhea, and even for headaches and fever. However, the mechanisms underlying the anti-proliferative activity of PC in endometrial cancer (EC) cells have yet to be fully elucidated. This study investigated the anticancer effects of an aqueous extract of Pogostemon cablin (PCAE), specifically induced apoptosis in EC (Ishikawa) cells. Proliferation of EC cells following exposure to PCAE was assessed by an MTT assay. DNA content and the induction of cell cycle apoptosis were analyzed by flow cytometry (FACS Calibur). Protein caspase-3 and, -9 as well as AIF were investigated using Western blot. Our results demonstrate growth inhibition of Ishikawa cells by PCAE. Furthermore, caspase-3 activity caused PCAE-treated cell lines to accumulate in apoptosis. Gene expression profiling (GEP) results further suggest that, in addition to its known effects with regard to EC prevention, PCAE may also exert antitumor activity on established EC cells. Many previous studies have identified the chemo-preventive effects of natural plant materials and the potential role of these materials in chemotherapy. This current study used human EC Ishikawa cells to investigate the anti-tumor effects of PCAE in EC cells. Our results demonstrate that PCAE inhibits the growth of cancer cells and induces apoptosis, which suggests the potential applicability of PCAE as an antitumor agent.

No MeSH data available.


Related in: MedlinePlus

PCAE mediates the survival of EC cells through the inhibition of proliferation. Ishikawa cells were treated with increasing doses of PCAE (0, 1, 2 and 4 mg/mL) for 24 to 72 h in vitro. The survival of PCAE-treated cancer cells was measured using the MTT method. Results are expressed as a percentage of the control, which was considered to be 100%. All data are reported as the mean (±SEM) of at least three separate experiments. Statistical analysis was performed using a t-test, with significant differences (p < 0.05) between treatment and control (PCAE 0 mg/mL) groups, and & vs. the 24 h group and # vs. the 48 h group, delineated by the * symbol.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4490452&req=5

ijms-16-12424-f001: PCAE mediates the survival of EC cells through the inhibition of proliferation. Ishikawa cells were treated with increasing doses of PCAE (0, 1, 2 and 4 mg/mL) for 24 to 72 h in vitro. The survival of PCAE-treated cancer cells was measured using the MTT method. Results are expressed as a percentage of the control, which was considered to be 100%. All data are reported as the mean (±SEM) of at least three separate experiments. Statistical analysis was performed using a t-test, with significant differences (p < 0.05) between treatment and control (PCAE 0 mg/mL) groups, and & vs. the 24 h group and # vs. the 48 h group, delineated by the * symbol.

Mentions: This study hypothesized that PCAE could mediate the survival of Ishikawa cells and thereby inhibit proliferation. The anti-tumor activity of PCAE against Ishikawa cells was investigated in vitro by treating Ishikawa cells with increasing doses of PCAE (0, 1, 2, and 4 mg/mL) over a period of 24 to 72 h. We then measured the proliferation of PCAE-treated cancer cells using the MTT method, the results of which are summarized in Figure 1. Our findings indicate that the survival and proliferation of Ishikawa cells decreased with an increase in the dose of PCAE (y = −14.306 x + 124.02 R2 = 0.6902). A microscopic examination further revealed morphological changes in Ishikawa cells following exposure to PCAE (2 mg/mL) for 6 to 24 h, and PCAE was also shown to induce the death of cancer cells, which resulted in a suspension of dead cells in the medium (data not shown).


Induction of Apoptosis in Endometrial Cancer (Ishikawa) Cells by Pogostemon cablin Aqueous Extract (PCAE).

Tsai CC, Chang YH, Chang CC, Cheng YM, Ou YC, Chien CC, Hsu YC - Int J Mol Sci (2015)

PCAE mediates the survival of EC cells through the inhibition of proliferation. Ishikawa cells were treated with increasing doses of PCAE (0, 1, 2 and 4 mg/mL) for 24 to 72 h in vitro. The survival of PCAE-treated cancer cells was measured using the MTT method. Results are expressed as a percentage of the control, which was considered to be 100%. All data are reported as the mean (±SEM) of at least three separate experiments. Statistical analysis was performed using a t-test, with significant differences (p < 0.05) between treatment and control (PCAE 0 mg/mL) groups, and & vs. the 24 h group and # vs. the 48 h group, delineated by the * symbol.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490452&req=5

ijms-16-12424-f001: PCAE mediates the survival of EC cells through the inhibition of proliferation. Ishikawa cells were treated with increasing doses of PCAE (0, 1, 2 and 4 mg/mL) for 24 to 72 h in vitro. The survival of PCAE-treated cancer cells was measured using the MTT method. Results are expressed as a percentage of the control, which was considered to be 100%. All data are reported as the mean (±SEM) of at least three separate experiments. Statistical analysis was performed using a t-test, with significant differences (p < 0.05) between treatment and control (PCAE 0 mg/mL) groups, and & vs. the 24 h group and # vs. the 48 h group, delineated by the * symbol.
Mentions: This study hypothesized that PCAE could mediate the survival of Ishikawa cells and thereby inhibit proliferation. The anti-tumor activity of PCAE against Ishikawa cells was investigated in vitro by treating Ishikawa cells with increasing doses of PCAE (0, 1, 2, and 4 mg/mL) over a period of 24 to 72 h. We then measured the proliferation of PCAE-treated cancer cells using the MTT method, the results of which are summarized in Figure 1. Our findings indicate that the survival and proliferation of Ishikawa cells decreased with an increase in the dose of PCAE (y = −14.306 x + 124.02 R2 = 0.6902). A microscopic examination further revealed morphological changes in Ishikawa cells following exposure to PCAE (2 mg/mL) for 6 to 24 h, and PCAE was also shown to induce the death of cancer cells, which resulted in a suspension of dead cells in the medium (data not shown).

Bottom Line: Gene expression profiling (GEP) results further suggest that, in addition to its known effects with regard to EC prevention, PCAE may also exert antitumor activity on established EC cells.Many previous studies have identified the chemo-preventive effects of natural plant materials and the potential role of these materials in chemotherapy.This current study used human EC Ishikawa cells to investigate the anti-tumor effects of PCAE in EC cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Kaohsiung 83301, Taiwan. nick@adm.cgmh.org.tw.

ABSTRACT
Pogostemon cablin (PC) is a traditional herbal medicine used in the treatment of the common cold, nausea, diarrhea, and even for headaches and fever. However, the mechanisms underlying the anti-proliferative activity of PC in endometrial cancer (EC) cells have yet to be fully elucidated. This study investigated the anticancer effects of an aqueous extract of Pogostemon cablin (PCAE), specifically induced apoptosis in EC (Ishikawa) cells. Proliferation of EC cells following exposure to PCAE was assessed by an MTT assay. DNA content and the induction of cell cycle apoptosis were analyzed by flow cytometry (FACS Calibur). Protein caspase-3 and, -9 as well as AIF were investigated using Western blot. Our results demonstrate growth inhibition of Ishikawa cells by PCAE. Furthermore, caspase-3 activity caused PCAE-treated cell lines to accumulate in apoptosis. Gene expression profiling (GEP) results further suggest that, in addition to its known effects with regard to EC prevention, PCAE may also exert antitumor activity on established EC cells. Many previous studies have identified the chemo-preventive effects of natural plant materials and the potential role of these materials in chemotherapy. This current study used human EC Ishikawa cells to investigate the anti-tumor effects of PCAE in EC cells. Our results demonstrate that PCAE inhibits the growth of cancer cells and induces apoptosis, which suggests the potential applicability of PCAE as an antitumor agent.

No MeSH data available.


Related in: MedlinePlus