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The Arginine/ADMA Ratio Is Related to the Prevention of Atherosclerotic Plaques in Hypercholesterolemic Rabbits When Giving a Combined Therapy with Atorvastatine and Arginine.

Brinkmann SJ, Wörner EA, Buijs N, Richir M, Cynober L, van Leeuwen PA, Couderc R - Int J Mol Sci (2015)

Bottom Line: The decrease in ADMA levels over time was significantly correlated to fewer aortic lesions in the distal aorta and total aorta.The arginine/ADMA ratio was correlated to cholesterol levels and decrease in cholesterol levels over time in the SA group.A lower arginine/ADMA ratio was significantly correlated to lower NO levels in the S and C group.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, VU University Medical Center, 1081 HV Amsterdam, The Netherlands. sj.brinkmann@vumc.nl.

ABSTRACT

Unlabelled: Supplementation with arginine in combination with atorvastatin is more efficient in reducing the size of an atherosclerotic plaque than treatment with a statin or arginine alone in homozygous Watanabe heritable hyperlipidemic (WHHL) rabbits. We evaluated the mechanism behind this feature by exploring the role of the arginine/asymmetric dimethylarginine (ADMA) ratio, which is the substrate and inhibitor of nitric oxide synthase (NOS) and thereby nitric oxide (NO), respectively.

Methods: Rabbits were fed either an arginine diet (group A, n = 9), standard rabbit chow plus atorvastatin (group S, n = 8), standard rabbit chow plus an arginine diet with atorvastatin (group SA, n = 8) or standard rabbit chow (group C, n = 9) as control. Blood was sampled and the aorta was harvested for topographic and histological analysis. Plasma levels of arginine, ADMA, cholesterol and nitric oxide were determined and the arginine/ADMA ratio was calculated.

Results: The decrease in ADMA levels over time was significantly correlated to fewer aortic lesions in the distal aorta and total aorta. The arginine/ADMA ratio was correlated to cholesterol levels and decrease in cholesterol levels over time in the SA group. A lower arginine/ADMA ratio was significantly correlated to lower NO levels in the S and C group.

Discussion: A balance between arginine and ADMA is an important indicator in the prevention of the development of atherosclerotic plaques.

No MeSH data available.


Related in: MedlinePlus

Pearson correlation between arginine/ADMA ratio T8 and NO levels at T8 in the statine (n = 8) and control group (n = 9) (S: r = 0.709, p = 0.049, C: r = 0.694, p = 0.056).
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ijms-16-12230-f004: Pearson correlation between arginine/ADMA ratio T8 and NO levels at T8 in the statine (n = 8) and control group (n = 9) (S: r = 0.709, p = 0.049, C: r = 0.694, p = 0.056).

Mentions: At T0, no significant difference between groups was found in arginine/ADMA ratio levels. The ratio was significantly increased at T8 in group A and SA (p < 0.05). A Pearson’s correlation test revealed the correlation between the arginine/ADMA ratio and cholesterol levels at T8, most pronounced in the SA group (r = −0.462). The arginine/ADMA ratio and cholesterol levels at T8 correlated positively (r = 0.279) in group A. In addition, the decrease in cholesterol over time was strongly correlated to the arginine/ADMA ratio in the S and SA group (S: r = 0.461, SA: 0.699) (see Figure 3). A lower arginine/ADMA ratio was significantly correlated to lower NO levels in the S and C group (S: r = 0.709, p = 0.049, C: r = 0.697, p = 0.056) (see Figure 4).


The Arginine/ADMA Ratio Is Related to the Prevention of Atherosclerotic Plaques in Hypercholesterolemic Rabbits When Giving a Combined Therapy with Atorvastatine and Arginine.

Brinkmann SJ, Wörner EA, Buijs N, Richir M, Cynober L, van Leeuwen PA, Couderc R - Int J Mol Sci (2015)

Pearson correlation between arginine/ADMA ratio T8 and NO levels at T8 in the statine (n = 8) and control group (n = 9) (S: r = 0.709, p = 0.049, C: r = 0.694, p = 0.056).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490441&req=5

ijms-16-12230-f004: Pearson correlation between arginine/ADMA ratio T8 and NO levels at T8 in the statine (n = 8) and control group (n = 9) (S: r = 0.709, p = 0.049, C: r = 0.694, p = 0.056).
Mentions: At T0, no significant difference between groups was found in arginine/ADMA ratio levels. The ratio was significantly increased at T8 in group A and SA (p < 0.05). A Pearson’s correlation test revealed the correlation between the arginine/ADMA ratio and cholesterol levels at T8, most pronounced in the SA group (r = −0.462). The arginine/ADMA ratio and cholesterol levels at T8 correlated positively (r = 0.279) in group A. In addition, the decrease in cholesterol over time was strongly correlated to the arginine/ADMA ratio in the S and SA group (S: r = 0.461, SA: 0.699) (see Figure 3). A lower arginine/ADMA ratio was significantly correlated to lower NO levels in the S and C group (S: r = 0.709, p = 0.049, C: r = 0.697, p = 0.056) (see Figure 4).

Bottom Line: The decrease in ADMA levels over time was significantly correlated to fewer aortic lesions in the distal aorta and total aorta.The arginine/ADMA ratio was correlated to cholesterol levels and decrease in cholesterol levels over time in the SA group.A lower arginine/ADMA ratio was significantly correlated to lower NO levels in the S and C group.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, VU University Medical Center, 1081 HV Amsterdam, The Netherlands. sj.brinkmann@vumc.nl.

ABSTRACT

Unlabelled: Supplementation with arginine in combination with atorvastatin is more efficient in reducing the size of an atherosclerotic plaque than treatment with a statin or arginine alone in homozygous Watanabe heritable hyperlipidemic (WHHL) rabbits. We evaluated the mechanism behind this feature by exploring the role of the arginine/asymmetric dimethylarginine (ADMA) ratio, which is the substrate and inhibitor of nitric oxide synthase (NOS) and thereby nitric oxide (NO), respectively.

Methods: Rabbits were fed either an arginine diet (group A, n = 9), standard rabbit chow plus atorvastatin (group S, n = 8), standard rabbit chow plus an arginine diet with atorvastatin (group SA, n = 8) or standard rabbit chow (group C, n = 9) as control. Blood was sampled and the aorta was harvested for topographic and histological analysis. Plasma levels of arginine, ADMA, cholesterol and nitric oxide were determined and the arginine/ADMA ratio was calculated.

Results: The decrease in ADMA levels over time was significantly correlated to fewer aortic lesions in the distal aorta and total aorta. The arginine/ADMA ratio was correlated to cholesterol levels and decrease in cholesterol levels over time in the SA group. A lower arginine/ADMA ratio was significantly correlated to lower NO levels in the S and C group.

Discussion: A balance between arginine and ADMA is an important indicator in the prevention of the development of atherosclerotic plaques.

No MeSH data available.


Related in: MedlinePlus