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The Arginine/ADMA Ratio Is Related to the Prevention of Atherosclerotic Plaques in Hypercholesterolemic Rabbits When Giving a Combined Therapy with Atorvastatine and Arginine.

Brinkmann SJ, Wörner EA, Buijs N, Richir M, Cynober L, van Leeuwen PA, Couderc R - Int J Mol Sci (2015)

Bottom Line: The decrease in ADMA levels over time was significantly correlated to fewer aortic lesions in the distal aorta and total aorta.The arginine/ADMA ratio was correlated to cholesterol levels and decrease in cholesterol levels over time in the SA group.A lower arginine/ADMA ratio was significantly correlated to lower NO levels in the S and C group.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, VU University Medical Center, 1081 HV Amsterdam, The Netherlands. sj.brinkmann@vumc.nl.

ABSTRACT

Unlabelled: Supplementation with arginine in combination with atorvastatin is more efficient in reducing the size of an atherosclerotic plaque than treatment with a statin or arginine alone in homozygous Watanabe heritable hyperlipidemic (WHHL) rabbits. We evaluated the mechanism behind this feature by exploring the role of the arginine/asymmetric dimethylarginine (ADMA) ratio, which is the substrate and inhibitor of nitric oxide synthase (NOS) and thereby nitric oxide (NO), respectively.

Methods: Rabbits were fed either an arginine diet (group A, n = 9), standard rabbit chow plus atorvastatin (group S, n = 8), standard rabbit chow plus an arginine diet with atorvastatin (group SA, n = 8) or standard rabbit chow (group C, n = 9) as control. Blood was sampled and the aorta was harvested for topographic and histological analysis. Plasma levels of arginine, ADMA, cholesterol and nitric oxide were determined and the arginine/ADMA ratio was calculated.

Results: The decrease in ADMA levels over time was significantly correlated to fewer aortic lesions in the distal aorta and total aorta. The arginine/ADMA ratio was correlated to cholesterol levels and decrease in cholesterol levels over time in the SA group. A lower arginine/ADMA ratio was significantly correlated to lower NO levels in the S and C group.

Discussion: A balance between arginine and ADMA is an important indicator in the prevention of the development of atherosclerotic plaques.

No MeSH data available.


Related in: MedlinePlus

Pearson correlation between ∆ADMA (T0–T8, n = 4 per group) and aortic lesions in the distal aorta (r = 0.677, p = 0.01) and total aorta (r = 0.599, p = 0.03).
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ijms-16-12230-f002: Pearson correlation between ∆ADMA (T0–T8, n = 4 per group) and aortic lesions in the distal aorta (r = 0.677, p = 0.01) and total aorta (r = 0.599, p = 0.03).

Mentions: At T0 and T8, ADMA and NO levels did not significantly differ between groups. At the end of treatment (T8) ADMA levels decreased in all groups, but not significantly. The decrease in ADMA levels over time (T0–T8), when analyzing all groups together, showed to be significantly correlated to less aortic lesions in the distal aorta (r = 0.677, p = 0.01) and total aorta (r = 0.599, p = 0.03). Thus, the bigger the decrease in ADMA levels over time, the smaller the amount of arteriosclerotic lesions in the distal aorta (see Figure 2).


The Arginine/ADMA Ratio Is Related to the Prevention of Atherosclerotic Plaques in Hypercholesterolemic Rabbits When Giving a Combined Therapy with Atorvastatine and Arginine.

Brinkmann SJ, Wörner EA, Buijs N, Richir M, Cynober L, van Leeuwen PA, Couderc R - Int J Mol Sci (2015)

Pearson correlation between ∆ADMA (T0–T8, n = 4 per group) and aortic lesions in the distal aorta (r = 0.677, p = 0.01) and total aorta (r = 0.599, p = 0.03).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490441&req=5

ijms-16-12230-f002: Pearson correlation between ∆ADMA (T0–T8, n = 4 per group) and aortic lesions in the distal aorta (r = 0.677, p = 0.01) and total aorta (r = 0.599, p = 0.03).
Mentions: At T0 and T8, ADMA and NO levels did not significantly differ between groups. At the end of treatment (T8) ADMA levels decreased in all groups, but not significantly. The decrease in ADMA levels over time (T0–T8), when analyzing all groups together, showed to be significantly correlated to less aortic lesions in the distal aorta (r = 0.677, p = 0.01) and total aorta (r = 0.599, p = 0.03). Thus, the bigger the decrease in ADMA levels over time, the smaller the amount of arteriosclerotic lesions in the distal aorta (see Figure 2).

Bottom Line: The decrease in ADMA levels over time was significantly correlated to fewer aortic lesions in the distal aorta and total aorta.The arginine/ADMA ratio was correlated to cholesterol levels and decrease in cholesterol levels over time in the SA group.A lower arginine/ADMA ratio was significantly correlated to lower NO levels in the S and C group.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, VU University Medical Center, 1081 HV Amsterdam, The Netherlands. sj.brinkmann@vumc.nl.

ABSTRACT

Unlabelled: Supplementation with arginine in combination with atorvastatin is more efficient in reducing the size of an atherosclerotic plaque than treatment with a statin or arginine alone in homozygous Watanabe heritable hyperlipidemic (WHHL) rabbits. We evaluated the mechanism behind this feature by exploring the role of the arginine/asymmetric dimethylarginine (ADMA) ratio, which is the substrate and inhibitor of nitric oxide synthase (NOS) and thereby nitric oxide (NO), respectively.

Methods: Rabbits were fed either an arginine diet (group A, n = 9), standard rabbit chow plus atorvastatin (group S, n = 8), standard rabbit chow plus an arginine diet with atorvastatin (group SA, n = 8) or standard rabbit chow (group C, n = 9) as control. Blood was sampled and the aorta was harvested for topographic and histological analysis. Plasma levels of arginine, ADMA, cholesterol and nitric oxide were determined and the arginine/ADMA ratio was calculated.

Results: The decrease in ADMA levels over time was significantly correlated to fewer aortic lesions in the distal aorta and total aorta. The arginine/ADMA ratio was correlated to cholesterol levels and decrease in cholesterol levels over time in the SA group. A lower arginine/ADMA ratio was significantly correlated to lower NO levels in the S and C group.

Discussion: A balance between arginine and ADMA is an important indicator in the prevention of the development of atherosclerotic plaques.

No MeSH data available.


Related in: MedlinePlus