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Synergistic and Antagonistic Action of Phytochrome (Phy) A and PhyB during Seedling De-Etiolation in Arabidopsis thaliana.

Su L, Hou P, Song M, Zheng X, Guo L, Xiao Y, Yan L, Li W, Yang J - Int J Mol Sci (2015)

Bottom Line: In this work, we compared hypocotyl elongation of the phyA-211 phyB-9 double mutant with the wild type, the phyA-211 and phyB-9 single mutants under different intensities of far-red (FR), red (R), blue (B) and white (W) light.We confirmed that phyA and phyB synergistically promote seedling de-etiolation in B-, B plus R-, W- and high R-light conditions.Gene expression analyses of RBCS members and HY5 suggest that phyB and phyA act antagonistically on seedling development under FR light.

View Article: PubMed Central - PubMed

Affiliation: Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China. suliang_mp5@163.com.

ABSTRACT
It has been reported that Arabidopsis phytochrome (phy) A and phyB are crucial photoreceptors that display synergistic and antagonistic action during seedling de-etiolation in multiple light signaling pathways. However, the functional relationship between phyA and phyB is not fully understood under different kinds of light and in response to different intensities of such light. In this work, we compared hypocotyl elongation of the phyA-211 phyB-9 double mutant with the wild type, the phyA-211 and phyB-9 single mutants under different intensities of far-red (FR), red (R), blue (B) and white (W) light. We confirmed that phyA and phyB synergistically promote seedling de-etiolation in B-, B plus R-, W- and high R-light conditions. The correlation of endogenous ELONGATED HYPOCOTYL 5 (HY5) protein levels with the trend of hypocotyl elongation of all lines indicate that both phyA and phyB promote seedling photomorphogenesis in a synergistic manner in high-irradiance white light. Gene expression analyses of RBCS members and HY5 suggest that phyB and phyA act antagonistically on seedling development under FR light.

No MeSH data available.


Related in: MedlinePlus

PhyA and phyB synergistically repress hypocotyl elongation under blue (B) light condition; (A) Morphology of the WT Col-0, phyA-211, phyB-9 and phyA-211 phyB-9 grown under B light (142 μmol·m−2·s−1) for four days. Bar = 2 mm; (B) Quantification of hypocotyl lengths of seedlings shown in A. The means of three replicates (at least 30 seedlings each replicate) are shown ± SE; (C) PhyB shows a synergistic effect with phyA to promote seedling de-etiolation responses under different intensities of B light. The means of three replicates (at least 30 seedlings each replicate) are shown ± SE; and (D) PhyA and phyB synergistically promote seedling de-etiolation under different intensities of R plus B light. The means of three replicates (at least 30 seedlings each replicate) are shown ± SE.
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ijms-16-12199-f002: PhyA and phyB synergistically repress hypocotyl elongation under blue (B) light condition; (A) Morphology of the WT Col-0, phyA-211, phyB-9 and phyA-211 phyB-9 grown under B light (142 μmol·m−2·s−1) for four days. Bar = 2 mm; (B) Quantification of hypocotyl lengths of seedlings shown in A. The means of three replicates (at least 30 seedlings each replicate) are shown ± SE; (C) PhyB shows a synergistic effect with phyA to promote seedling de-etiolation responses under different intensities of B light. The means of three replicates (at least 30 seedlings each replicate) are shown ± SE; and (D) PhyA and phyB synergistically promote seedling de-etiolation under different intensities of R plus B light. The means of three replicates (at least 30 seedlings each replicate) are shown ± SE.

Mentions: Cryptochromes (CRY), including CRY1 and CRY2, are major photoreceptors mediating B light signaling [5]. Under the B light condition, the phyA mutant exhibited a longer hypocotyl than the WT, and the phyA cry1, phyB cry1 and phyAphyB double mutants had longer hypocotyls than those of cry1 or phyA single mutants, respectively, suggesting that both phyA and phyB are involved in B light signaling [12,22,23]. Although less difference in hypocotyl length was observed between the phyB-9 mutant and the WT, the hypocotyl length of the phyA-211 phyB-9 double mutant was much longer than that of either the phyA-211 or phyB-9 mutant (Figure 2A,B). These results suggest that a mutual promotion between phyA and phyB may be responsible for the excessive hypocotyl elongation of the phyA-211 phyB-9 double mutant in response to B light.


Synergistic and Antagonistic Action of Phytochrome (Phy) A and PhyB during Seedling De-Etiolation in Arabidopsis thaliana.

Su L, Hou P, Song M, Zheng X, Guo L, Xiao Y, Yan L, Li W, Yang J - Int J Mol Sci (2015)

PhyA and phyB synergistically repress hypocotyl elongation under blue (B) light condition; (A) Morphology of the WT Col-0, phyA-211, phyB-9 and phyA-211 phyB-9 grown under B light (142 μmol·m−2·s−1) for four days. Bar = 2 mm; (B) Quantification of hypocotyl lengths of seedlings shown in A. The means of three replicates (at least 30 seedlings each replicate) are shown ± SE; (C) PhyB shows a synergistic effect with phyA to promote seedling de-etiolation responses under different intensities of B light. The means of three replicates (at least 30 seedlings each replicate) are shown ± SE; and (D) PhyA and phyB synergistically promote seedling de-etiolation under different intensities of R plus B light. The means of three replicates (at least 30 seedlings each replicate) are shown ± SE.
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Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4490439&req=5

ijms-16-12199-f002: PhyA and phyB synergistically repress hypocotyl elongation under blue (B) light condition; (A) Morphology of the WT Col-0, phyA-211, phyB-9 and phyA-211 phyB-9 grown under B light (142 μmol·m−2·s−1) for four days. Bar = 2 mm; (B) Quantification of hypocotyl lengths of seedlings shown in A. The means of three replicates (at least 30 seedlings each replicate) are shown ± SE; (C) PhyB shows a synergistic effect with phyA to promote seedling de-etiolation responses under different intensities of B light. The means of three replicates (at least 30 seedlings each replicate) are shown ± SE; and (D) PhyA and phyB synergistically promote seedling de-etiolation under different intensities of R plus B light. The means of three replicates (at least 30 seedlings each replicate) are shown ± SE.
Mentions: Cryptochromes (CRY), including CRY1 and CRY2, are major photoreceptors mediating B light signaling [5]. Under the B light condition, the phyA mutant exhibited a longer hypocotyl than the WT, and the phyA cry1, phyB cry1 and phyAphyB double mutants had longer hypocotyls than those of cry1 or phyA single mutants, respectively, suggesting that both phyA and phyB are involved in B light signaling [12,22,23]. Although less difference in hypocotyl length was observed between the phyB-9 mutant and the WT, the hypocotyl length of the phyA-211 phyB-9 double mutant was much longer than that of either the phyA-211 or phyB-9 mutant (Figure 2A,B). These results suggest that a mutual promotion between phyA and phyB may be responsible for the excessive hypocotyl elongation of the phyA-211 phyB-9 double mutant in response to B light.

Bottom Line: In this work, we compared hypocotyl elongation of the phyA-211 phyB-9 double mutant with the wild type, the phyA-211 and phyB-9 single mutants under different intensities of far-red (FR), red (R), blue (B) and white (W) light.We confirmed that phyA and phyB synergistically promote seedling de-etiolation in B-, B plus R-, W- and high R-light conditions.Gene expression analyses of RBCS members and HY5 suggest that phyB and phyA act antagonistically on seedling development under FR light.

View Article: PubMed Central - PubMed

Affiliation: Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China. suliang_mp5@163.com.

ABSTRACT
It has been reported that Arabidopsis phytochrome (phy) A and phyB are crucial photoreceptors that display synergistic and antagonistic action during seedling de-etiolation in multiple light signaling pathways. However, the functional relationship between phyA and phyB is not fully understood under different kinds of light and in response to different intensities of such light. In this work, we compared hypocotyl elongation of the phyA-211 phyB-9 double mutant with the wild type, the phyA-211 and phyB-9 single mutants under different intensities of far-red (FR), red (R), blue (B) and white (W) light. We confirmed that phyA and phyB synergistically promote seedling de-etiolation in B-, B plus R-, W- and high R-light conditions. The correlation of endogenous ELONGATED HYPOCOTYL 5 (HY5) protein levels with the trend of hypocotyl elongation of all lines indicate that both phyA and phyB promote seedling photomorphogenesis in a synergistic manner in high-irradiance white light. Gene expression analyses of RBCS members and HY5 suggest that phyB and phyA act antagonistically on seedling development under FR light.

No MeSH data available.


Related in: MedlinePlus