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Functional Analysis of the Maize C-Repeat/DRE Motif-Binding Transcription Factor CBF3 Promoter in Response to Abiotic Stress.

Liu J, Wang F, Yu G, Zhang X, Jia C, Qin J, Pan H - Int J Mol Sci (2015)

Bottom Line: PZmCBF3 was activated by cold stress.The MYCCONSENSUSAT elements (CANNTG) were responsible for the ability of PZmCBF3 to respond to cold stress.The results of the present study suggest that PZmCBF3 might play a role in cold tolerance in maize.

View Article: PubMed Central - PubMed

Affiliation: College of Plant Sciences, Jilin University, Changchun 130062, China. jlliu@jlu.edu.cn.

ABSTRACT
The ZmCBF3 gene is a member of AP2/ERF transcription factor family, which is a large family of plant-specific transcription factors that share a well-conserved DNA-binding domain. To understand the regulatory mechanism of ZmCBF3 gene expression, we isolated and characterized the ZmCBF3 promoter (PZmCBF3). Three deletion fragments of PZmCBF3 were generated, C1-C3, from the translation start codon at position -1079, -638, and -234, and fused to the GUS reporter gene. Each deletion construct was analyzed by Agrobacterium-mediated stable transformation and expression in Arabidopsis thaliana. GUS expression assays indicated that the PZmCBF3 exhibited root-specific expression activity. A 234-bp fragment upstream of the ZmCBF3 gene conferred a high level of GUS activity in Arabidopsis. Some cis-acting elements involved in the down-regulation of gene expression were detected in the promoter, encompassing positions -1079 to -234. PZmCBF3 was activated by cold stress. The MYCCONSENSUSAT elements (CANNTG) were responsible for the ability of PZmCBF3 to respond to cold stress. The results of the present study suggest that PZmCBF3 might play a role in cold tolerance in maize.

No MeSH data available.


Related in: MedlinePlus

Nucleotide sequence and putative cis-acting elements of the ZmCBF3 promoter (PZmCBF3). The cis-acting elements are boxed, and arrows indicate their direction. The translation initiation codon is underlined.
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ijms-16-12131-f001: Nucleotide sequence and putative cis-acting elements of the ZmCBF3 promoter (PZmCBF3). The cis-acting elements are boxed, and arrows indicate their direction. The translation initiation codon is underlined.

Mentions: To characterize the promoter of ZmCBF3 (PZmCBF3), the 5′-upstream sequence was cloned from maize (B73) genomic DNA. The cloned DNA region was 1443 bp in length (Figure 1) and was analyzed by using the PLACE and the PlantCARE databases. The putative cis-acting elements were identified and labeled (Figure 1), which were homologous to the cis-acting elements of other plants.


Functional Analysis of the Maize C-Repeat/DRE Motif-Binding Transcription Factor CBF3 Promoter in Response to Abiotic Stress.

Liu J, Wang F, Yu G, Zhang X, Jia C, Qin J, Pan H - Int J Mol Sci (2015)

Nucleotide sequence and putative cis-acting elements of the ZmCBF3 promoter (PZmCBF3). The cis-acting elements are boxed, and arrows indicate their direction. The translation initiation codon is underlined.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490434&req=5

ijms-16-12131-f001: Nucleotide sequence and putative cis-acting elements of the ZmCBF3 promoter (PZmCBF3). The cis-acting elements are boxed, and arrows indicate their direction. The translation initiation codon is underlined.
Mentions: To characterize the promoter of ZmCBF3 (PZmCBF3), the 5′-upstream sequence was cloned from maize (B73) genomic DNA. The cloned DNA region was 1443 bp in length (Figure 1) and was analyzed by using the PLACE and the PlantCARE databases. The putative cis-acting elements were identified and labeled (Figure 1), which were homologous to the cis-acting elements of other plants.

Bottom Line: PZmCBF3 was activated by cold stress.The MYCCONSENSUSAT elements (CANNTG) were responsible for the ability of PZmCBF3 to respond to cold stress.The results of the present study suggest that PZmCBF3 might play a role in cold tolerance in maize.

View Article: PubMed Central - PubMed

Affiliation: College of Plant Sciences, Jilin University, Changchun 130062, China. jlliu@jlu.edu.cn.

ABSTRACT
The ZmCBF3 gene is a member of AP2/ERF transcription factor family, which is a large family of plant-specific transcription factors that share a well-conserved DNA-binding domain. To understand the regulatory mechanism of ZmCBF3 gene expression, we isolated and characterized the ZmCBF3 promoter (PZmCBF3). Three deletion fragments of PZmCBF3 were generated, C1-C3, from the translation start codon at position -1079, -638, and -234, and fused to the GUS reporter gene. Each deletion construct was analyzed by Agrobacterium-mediated stable transformation and expression in Arabidopsis thaliana. GUS expression assays indicated that the PZmCBF3 exhibited root-specific expression activity. A 234-bp fragment upstream of the ZmCBF3 gene conferred a high level of GUS activity in Arabidopsis. Some cis-acting elements involved in the down-regulation of gene expression were detected in the promoter, encompassing positions -1079 to -234. PZmCBF3 was activated by cold stress. The MYCCONSENSUSAT elements (CANNTG) were responsible for the ability of PZmCBF3 to respond to cold stress. The results of the present study suggest that PZmCBF3 might play a role in cold tolerance in maize.

No MeSH data available.


Related in: MedlinePlus