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Human adipose-derived mesenchymal progenitor cells engraft into rabbit articular cartilage.

Wang W, He N, Feng C, Liu V, Zhang L, Wang F, He J, Zhu T, Wang S, Qiao W, Li S, Zhou G, Zhang L, Dai C, Cao W - Int J Mol Sci (2015)

Bottom Line: The data showed that haMPC treatment promoted cartilage repair.Signals of human mitochondrial can be directly detected in haMPC treated cartilage.The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo.

View Article: PubMed Central - PubMed

Affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. maxwell.wang@cellbiomedgroup.com.

ABSTRACT
Mesenchymal stem cells (MSCs) are known to have the potential for articular cartilage regeneration, and are suggested for the treatment of osteoarthritis (OA). Here, we investigated whether intra-articular injection of xenogeneic human adipose-derived mesenchymal progenitor cells (haMPCs) promoted articular cartilage repair in rabbit OA model and engrafted into rabbit articular cartilage. The haMPCs were cultured in vitro, and phenotypes and differentiation characteristics of cells were evaluated. OA was induced surgically by anterior cruciate ligament transection (ACLT) and medical meniscectomy of knee joints. At six weeks following surgery, hyaluronic acid (HA) or haMPCs was injected into the knee joints, the contralateral knee served as normal control. All animals were sacrificed at the 16th week post-surgery. Assessments were carried out by macroscopic examination, hematoxylin/eosin (HE) and Safranin-O/Fast green stainings and immunohistochemistry. The data showed that haMPC treatment promoted cartilage repair. Signals of human mitochondrial can be directly detected in haMPC treated cartilage. The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo. These results suggest that intra-articular injection of haMPCs promotes regeneration of articular cartilage in rabbit OA model, and support the notion that MPCs are transplantable between HLA-incompatible individuals.

No MeSH data available.


Related in: MedlinePlus

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ijms-16-12076-f008: Experimental scheme.

Mentions: Adult male New Zealand white rabbits (n = 12) weighing 2.5 ± 0.5 kg were used. All animals were induced OA by ACLT and medial meniscectomy of right knee. The animals were divided randomly into two groups according to the subsequent intra-articular injections which they received: HA group as vehicle control (n = 6) and haMPC group (n = 6), and the contralateral knee served as normal group. The overview of the total experimental design was shown in Figure 8. All animal experimental protocol was approved by Ethical Committee of Shanghai Medical College, Fudan University.


Human adipose-derived mesenchymal progenitor cells engraft into rabbit articular cartilage.

Wang W, He N, Feng C, Liu V, Zhang L, Wang F, He J, Zhu T, Wang S, Qiao W, Li S, Zhou G, Zhang L, Dai C, Cao W - Int J Mol Sci (2015)

Experimental scheme.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490430&req=5

ijms-16-12076-f008: Experimental scheme.
Mentions: Adult male New Zealand white rabbits (n = 12) weighing 2.5 ± 0.5 kg were used. All animals were induced OA by ACLT and medial meniscectomy of right knee. The animals were divided randomly into two groups according to the subsequent intra-articular injections which they received: HA group as vehicle control (n = 6) and haMPC group (n = 6), and the contralateral knee served as normal group. The overview of the total experimental design was shown in Figure 8. All animal experimental protocol was approved by Ethical Committee of Shanghai Medical College, Fudan University.

Bottom Line: The data showed that haMPC treatment promoted cartilage repair.Signals of human mitochondrial can be directly detected in haMPC treated cartilage.The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo.

View Article: PubMed Central - PubMed

Affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. maxwell.wang@cellbiomedgroup.com.

ABSTRACT
Mesenchymal stem cells (MSCs) are known to have the potential for articular cartilage regeneration, and are suggested for the treatment of osteoarthritis (OA). Here, we investigated whether intra-articular injection of xenogeneic human adipose-derived mesenchymal progenitor cells (haMPCs) promoted articular cartilage repair in rabbit OA model and engrafted into rabbit articular cartilage. The haMPCs were cultured in vitro, and phenotypes and differentiation characteristics of cells were evaluated. OA was induced surgically by anterior cruciate ligament transection (ACLT) and medical meniscectomy of knee joints. At six weeks following surgery, hyaluronic acid (HA) or haMPCs was injected into the knee joints, the contralateral knee served as normal control. All animals were sacrificed at the 16th week post-surgery. Assessments were carried out by macroscopic examination, hematoxylin/eosin (HE) and Safranin-O/Fast green stainings and immunohistochemistry. The data showed that haMPC treatment promoted cartilage repair. Signals of human mitochondrial can be directly detected in haMPC treated cartilage. The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo. These results suggest that intra-articular injection of haMPCs promotes regeneration of articular cartilage in rabbit OA model, and support the notion that MPCs are transplantable between HLA-incompatible individuals.

No MeSH data available.


Related in: MedlinePlus