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Human adipose-derived mesenchymal progenitor cells engraft into rabbit articular cartilage.

Wang W, He N, Feng C, Liu V, Zhang L, Wang F, He J, Zhu T, Wang S, Qiao W, Li S, Zhou G, Zhang L, Dai C, Cao W - Int J Mol Sci (2015)

Bottom Line: The data showed that haMPC treatment promoted cartilage repair.Signals of human mitochondrial can be directly detected in haMPC treated cartilage.The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo.

View Article: PubMed Central - PubMed

Affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. maxwell.wang@cellbiomedgroup.com.

ABSTRACT
Mesenchymal stem cells (MSCs) are known to have the potential for articular cartilage regeneration, and are suggested for the treatment of osteoarthritis (OA). Here, we investigated whether intra-articular injection of xenogeneic human adipose-derived mesenchymal progenitor cells (haMPCs) promoted articular cartilage repair in rabbit OA model and engrafted into rabbit articular cartilage. The haMPCs were cultured in vitro, and phenotypes and differentiation characteristics of cells were evaluated. OA was induced surgically by anterior cruciate ligament transection (ACLT) and medical meniscectomy of knee joints. At six weeks following surgery, hyaluronic acid (HA) or haMPCs was injected into the knee joints, the contralateral knee served as normal control. All animals were sacrificed at the 16th week post-surgery. Assessments were carried out by macroscopic examination, hematoxylin/eosin (HE) and Safranin-O/Fast green stainings and immunohistochemistry. The data showed that haMPC treatment promoted cartilage repair. Signals of human mitochondrial can be directly detected in haMPC treated cartilage. The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo. These results suggest that intra-articular injection of haMPCs promotes regeneration of articular cartilage in rabbit OA model, and support the notion that MPCs are transplantable between HLA-incompatible individuals.

No MeSH data available.


Related in: MedlinePlus

HLA-I but not HLA-II-DR was expressed in haMPC treatment group. In normal and HA treated cartilage, HLA-I and HLA-II-DR were negative. HLA-I but not HLA-II-DR was expressed in haMPC treated cartilage. Immunohistochemical analyses of HLA-I and HLA-II-DR in human tonsil tissue were used as positive control and negative (isotype) control. Scale bars = 50 μm.
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ijms-16-12076-f007: HLA-I but not HLA-II-DR was expressed in haMPC treatment group. In normal and HA treated cartilage, HLA-I and HLA-II-DR were negative. HLA-I but not HLA-II-DR was expressed in haMPC treated cartilage. Immunohistochemical analyses of HLA-I and HLA-II-DR in human tonsil tissue were used as positive control and negative (isotype) control. Scale bars = 50 μm.

Mentions: Since we found haMPCs engraft into rabbit cartilage, the next step we want to know whether engrafted haMPCs express HLA-I and HLA-II-DR in vivo. Using anti-human monoclonal antibodies, we found engrafted haMPCs express HLA-I but not HLA-II-DR (Figure 7), which are consistent with in vitro phenotypic data (Figure 1). Moreover, HLA-I positive cells locate at the superficial area of rabbit cartilage consistent with engraftment data (Figure 7).


Human adipose-derived mesenchymal progenitor cells engraft into rabbit articular cartilage.

Wang W, He N, Feng C, Liu V, Zhang L, Wang F, He J, Zhu T, Wang S, Qiao W, Li S, Zhou G, Zhang L, Dai C, Cao W - Int J Mol Sci (2015)

HLA-I but not HLA-II-DR was expressed in haMPC treatment group. In normal and HA treated cartilage, HLA-I and HLA-II-DR were negative. HLA-I but not HLA-II-DR was expressed in haMPC treated cartilage. Immunohistochemical analyses of HLA-I and HLA-II-DR in human tonsil tissue were used as positive control and negative (isotype) control. Scale bars = 50 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490430&req=5

ijms-16-12076-f007: HLA-I but not HLA-II-DR was expressed in haMPC treatment group. In normal and HA treated cartilage, HLA-I and HLA-II-DR were negative. HLA-I but not HLA-II-DR was expressed in haMPC treated cartilage. Immunohistochemical analyses of HLA-I and HLA-II-DR in human tonsil tissue were used as positive control and negative (isotype) control. Scale bars = 50 μm.
Mentions: Since we found haMPCs engraft into rabbit cartilage, the next step we want to know whether engrafted haMPCs express HLA-I and HLA-II-DR in vivo. Using anti-human monoclonal antibodies, we found engrafted haMPCs express HLA-I but not HLA-II-DR (Figure 7), which are consistent with in vitro phenotypic data (Figure 1). Moreover, HLA-I positive cells locate at the superficial area of rabbit cartilage consistent with engraftment data (Figure 7).

Bottom Line: The data showed that haMPC treatment promoted cartilage repair.Signals of human mitochondrial can be directly detected in haMPC treated cartilage.The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo.

View Article: PubMed Central - PubMed

Affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. maxwell.wang@cellbiomedgroup.com.

ABSTRACT
Mesenchymal stem cells (MSCs) are known to have the potential for articular cartilage regeneration, and are suggested for the treatment of osteoarthritis (OA). Here, we investigated whether intra-articular injection of xenogeneic human adipose-derived mesenchymal progenitor cells (haMPCs) promoted articular cartilage repair in rabbit OA model and engrafted into rabbit articular cartilage. The haMPCs were cultured in vitro, and phenotypes and differentiation characteristics of cells were evaluated. OA was induced surgically by anterior cruciate ligament transection (ACLT) and medical meniscectomy of knee joints. At six weeks following surgery, hyaluronic acid (HA) or haMPCs was injected into the knee joints, the contralateral knee served as normal control. All animals were sacrificed at the 16th week post-surgery. Assessments were carried out by macroscopic examination, hematoxylin/eosin (HE) and Safranin-O/Fast green stainings and immunohistochemistry. The data showed that haMPC treatment promoted cartilage repair. Signals of human mitochondrial can be directly detected in haMPC treated cartilage. The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo. These results suggest that intra-articular injection of haMPCs promotes regeneration of articular cartilage in rabbit OA model, and support the notion that MPCs are transplantable between HLA-incompatible individuals.

No MeSH data available.


Related in: MedlinePlus