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Human adipose-derived mesenchymal progenitor cells engraft into rabbit articular cartilage.

Wang W, He N, Feng C, Liu V, Zhang L, Wang F, He J, Zhu T, Wang S, Qiao W, Li S, Zhou G, Zhang L, Dai C, Cao W - Int J Mol Sci (2015)

Bottom Line: The data showed that haMPC treatment promoted cartilage repair.Signals of human mitochondrial can be directly detected in haMPC treated cartilage.The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo.

View Article: PubMed Central - PubMed

Affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. maxwell.wang@cellbiomedgroup.com.

ABSTRACT
Mesenchymal stem cells (MSCs) are known to have the potential for articular cartilage regeneration, and are suggested for the treatment of osteoarthritis (OA). Here, we investigated whether intra-articular injection of xenogeneic human adipose-derived mesenchymal progenitor cells (haMPCs) promoted articular cartilage repair in rabbit OA model and engrafted into rabbit articular cartilage. The haMPCs were cultured in vitro, and phenotypes and differentiation characteristics of cells were evaluated. OA was induced surgically by anterior cruciate ligament transection (ACLT) and medical meniscectomy of knee joints. At six weeks following surgery, hyaluronic acid (HA) or haMPCs was injected into the knee joints, the contralateral knee served as normal control. All animals were sacrificed at the 16th week post-surgery. Assessments were carried out by macroscopic examination, hematoxylin/eosin (HE) and Safranin-O/Fast green stainings and immunohistochemistry. The data showed that haMPC treatment promoted cartilage repair. Signals of human mitochondrial can be directly detected in haMPC treated cartilage. The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo. These results suggest that intra-articular injection of haMPCs promotes regeneration of articular cartilage in rabbit OA model, and support the notion that MPCs are transplantable between HLA-incompatible individuals.

No MeSH data available.


Related in: MedlinePlus

Histological findings. (A) HE and Safranin-O/Fast green stainings showed that haMPC treatment alleviated fissures and cracks formation; (B) The modified O’Driscoll histological score showed that haMPC treatment had significantly higher score compared with HA group; and (C) The haMPCs significantly increased cartilage thickness compared to HA group. Scale bars = 50 μm. * indicates p < 0.05; ** indicates p < 0.01.
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ijms-16-12076-f004: Histological findings. (A) HE and Safranin-O/Fast green stainings showed that haMPC treatment alleviated fissures and cracks formation; (B) The modified O’Driscoll histological score showed that haMPC treatment had significantly higher score compared with HA group; and (C) The haMPCs significantly increased cartilage thickness compared to HA group. Scale bars = 50 μm. * indicates p < 0.05; ** indicates p < 0.01.

Mentions: Histologically, in normal group, HE staining demonstrated that the surface of normal articular cartilage was smooth and had no fissures and cracks. Superficial zone, mid zone, deep zone, and calcified cartilage were all distinct. The superficial zone of cartilage and the tidemark between the deep zone of and calcified cartilage were intact and clear. In HA group, the articular cartilage had a rough border showing fibrillation formation and the superficial zone of chondrocytes was fragmentary. However, haMPC treatment revealed few fissures, few cracks, and almost continuous superficial zone (Figure 4A).


Human adipose-derived mesenchymal progenitor cells engraft into rabbit articular cartilage.

Wang W, He N, Feng C, Liu V, Zhang L, Wang F, He J, Zhu T, Wang S, Qiao W, Li S, Zhou G, Zhang L, Dai C, Cao W - Int J Mol Sci (2015)

Histological findings. (A) HE and Safranin-O/Fast green stainings showed that haMPC treatment alleviated fissures and cracks formation; (B) The modified O’Driscoll histological score showed that haMPC treatment had significantly higher score compared with HA group; and (C) The haMPCs significantly increased cartilage thickness compared to HA group. Scale bars = 50 μm. * indicates p < 0.05; ** indicates p < 0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490430&req=5

ijms-16-12076-f004: Histological findings. (A) HE and Safranin-O/Fast green stainings showed that haMPC treatment alleviated fissures and cracks formation; (B) The modified O’Driscoll histological score showed that haMPC treatment had significantly higher score compared with HA group; and (C) The haMPCs significantly increased cartilage thickness compared to HA group. Scale bars = 50 μm. * indicates p < 0.05; ** indicates p < 0.01.
Mentions: Histologically, in normal group, HE staining demonstrated that the surface of normal articular cartilage was smooth and had no fissures and cracks. Superficial zone, mid zone, deep zone, and calcified cartilage were all distinct. The superficial zone of cartilage and the tidemark between the deep zone of and calcified cartilage were intact and clear. In HA group, the articular cartilage had a rough border showing fibrillation formation and the superficial zone of chondrocytes was fragmentary. However, haMPC treatment revealed few fissures, few cracks, and almost continuous superficial zone (Figure 4A).

Bottom Line: The data showed that haMPC treatment promoted cartilage repair.Signals of human mitochondrial can be directly detected in haMPC treated cartilage.The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo.

View Article: PubMed Central - PubMed

Affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. maxwell.wang@cellbiomedgroup.com.

ABSTRACT
Mesenchymal stem cells (MSCs) are known to have the potential for articular cartilage regeneration, and are suggested for the treatment of osteoarthritis (OA). Here, we investigated whether intra-articular injection of xenogeneic human adipose-derived mesenchymal progenitor cells (haMPCs) promoted articular cartilage repair in rabbit OA model and engrafted into rabbit articular cartilage. The haMPCs were cultured in vitro, and phenotypes and differentiation characteristics of cells were evaluated. OA was induced surgically by anterior cruciate ligament transection (ACLT) and medical meniscectomy of knee joints. At six weeks following surgery, hyaluronic acid (HA) or haMPCs was injected into the knee joints, the contralateral knee served as normal control. All animals were sacrificed at the 16th week post-surgery. Assessments were carried out by macroscopic examination, hematoxylin/eosin (HE) and Safranin-O/Fast green stainings and immunohistochemistry. The data showed that haMPC treatment promoted cartilage repair. Signals of human mitochondrial can be directly detected in haMPC treated cartilage. The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo. These results suggest that intra-articular injection of haMPCs promotes regeneration of articular cartilage in rabbit OA model, and support the notion that MPCs are transplantable between HLA-incompatible individuals.

No MeSH data available.


Related in: MedlinePlus