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Human adipose-derived mesenchymal progenitor cells engraft into rabbit articular cartilage.

Wang W, He N, Feng C, Liu V, Zhang L, Wang F, He J, Zhu T, Wang S, Qiao W, Li S, Zhou G, Zhang L, Dai C, Cao W - Int J Mol Sci (2015)

Bottom Line: The data showed that haMPC treatment promoted cartilage repair.Signals of human mitochondrial can be directly detected in haMPC treated cartilage.The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo.

View Article: PubMed Central - PubMed

Affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. maxwell.wang@cellbiomedgroup.com.

ABSTRACT
Mesenchymal stem cells (MSCs) are known to have the potential for articular cartilage regeneration, and are suggested for the treatment of osteoarthritis (OA). Here, we investigated whether intra-articular injection of xenogeneic human adipose-derived mesenchymal progenitor cells (haMPCs) promoted articular cartilage repair in rabbit OA model and engrafted into rabbit articular cartilage. The haMPCs were cultured in vitro, and phenotypes and differentiation characteristics of cells were evaluated. OA was induced surgically by anterior cruciate ligament transection (ACLT) and medical meniscectomy of knee joints. At six weeks following surgery, hyaluronic acid (HA) or haMPCs was injected into the knee joints, the contralateral knee served as normal control. All animals were sacrificed at the 16th week post-surgery. Assessments were carried out by macroscopic examination, hematoxylin/eosin (HE) and Safranin-O/Fast green stainings and immunohistochemistry. The data showed that haMPC treatment promoted cartilage repair. Signals of human mitochondrial can be directly detected in haMPC treated cartilage. The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo. These results suggest that intra-articular injection of haMPCs promotes regeneration of articular cartilage in rabbit OA model, and support the notion that MPCs are transplantable between HLA-incompatible individuals.

No MeSH data available.


Related in: MedlinePlus

Macroscopic findings. (A) Sixteen weeks after surgery, in haMPC treatment group, eroded cartilage was almost completely covered by the repaired tissues (black arrow); and (B) Macroscopic OA ICRS scores showed haMPC treatment significantly increased ICRS scores compared with hyaluronic acid (HA) group. * indicates p < 0.05.
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ijms-16-12076-f003: Macroscopic findings. (A) Sixteen weeks after surgery, in haMPC treatment group, eroded cartilage was almost completely covered by the repaired tissues (black arrow); and (B) Macroscopic OA ICRS scores showed haMPC treatment significantly increased ICRS scores compared with hyaluronic acid (HA) group. * indicates p < 0.05.

Mentions: Macroscopically, 16 weeks after surgery, macroscopic evaluation exhibited that eroded cartilage of haMPC treatment was almost completely covered by the repaired tissues, and the repaired cartilage surface was relatively smooth. While large fissures and cracks were observed in hyaluronic acid (HA) treatment group as control compared with the cartilage of normal group (Figure 3A). The macroscopic OA ICRS scores were significantly higher in haMPC group than the HA group (Figure 3B).


Human adipose-derived mesenchymal progenitor cells engraft into rabbit articular cartilage.

Wang W, He N, Feng C, Liu V, Zhang L, Wang F, He J, Zhu T, Wang S, Qiao W, Li S, Zhou G, Zhang L, Dai C, Cao W - Int J Mol Sci (2015)

Macroscopic findings. (A) Sixteen weeks after surgery, in haMPC treatment group, eroded cartilage was almost completely covered by the repaired tissues (black arrow); and (B) Macroscopic OA ICRS scores showed haMPC treatment significantly increased ICRS scores compared with hyaluronic acid (HA) group. * indicates p < 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490430&req=5

ijms-16-12076-f003: Macroscopic findings. (A) Sixteen weeks after surgery, in haMPC treatment group, eroded cartilage was almost completely covered by the repaired tissues (black arrow); and (B) Macroscopic OA ICRS scores showed haMPC treatment significantly increased ICRS scores compared with hyaluronic acid (HA) group. * indicates p < 0.05.
Mentions: Macroscopically, 16 weeks after surgery, macroscopic evaluation exhibited that eroded cartilage of haMPC treatment was almost completely covered by the repaired tissues, and the repaired cartilage surface was relatively smooth. While large fissures and cracks were observed in hyaluronic acid (HA) treatment group as control compared with the cartilage of normal group (Figure 3A). The macroscopic OA ICRS scores were significantly higher in haMPC group than the HA group (Figure 3B).

Bottom Line: The data showed that haMPC treatment promoted cartilage repair.Signals of human mitochondrial can be directly detected in haMPC treated cartilage.The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo.

View Article: PubMed Central - PubMed

Affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. maxwell.wang@cellbiomedgroup.com.

ABSTRACT
Mesenchymal stem cells (MSCs) are known to have the potential for articular cartilage regeneration, and are suggested for the treatment of osteoarthritis (OA). Here, we investigated whether intra-articular injection of xenogeneic human adipose-derived mesenchymal progenitor cells (haMPCs) promoted articular cartilage repair in rabbit OA model and engrafted into rabbit articular cartilage. The haMPCs were cultured in vitro, and phenotypes and differentiation characteristics of cells were evaluated. OA was induced surgically by anterior cruciate ligament transection (ACLT) and medical meniscectomy of knee joints. At six weeks following surgery, hyaluronic acid (HA) or haMPCs was injected into the knee joints, the contralateral knee served as normal control. All animals were sacrificed at the 16th week post-surgery. Assessments were carried out by macroscopic examination, hematoxylin/eosin (HE) and Safranin-O/Fast green stainings and immunohistochemistry. The data showed that haMPC treatment promoted cartilage repair. Signals of human mitochondrial can be directly detected in haMPC treated cartilage. The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo. These results suggest that intra-articular injection of haMPCs promotes regeneration of articular cartilage in rabbit OA model, and support the notion that MPCs are transplantable between HLA-incompatible individuals.

No MeSH data available.


Related in: MedlinePlus