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Human adipose-derived mesenchymal progenitor cells engraft into rabbit articular cartilage.

Wang W, He N, Feng C, Liu V, Zhang L, Wang F, He J, Zhu T, Wang S, Qiao W, Li S, Zhou G, Zhang L, Dai C, Cao W - Int J Mol Sci (2015)

Bottom Line: The data showed that haMPC treatment promoted cartilage repair.Signals of human mitochondrial can be directly detected in haMPC treated cartilage.The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo.

View Article: PubMed Central - PubMed

Affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. maxwell.wang@cellbiomedgroup.com.

ABSTRACT
Mesenchymal stem cells (MSCs) are known to have the potential for articular cartilage regeneration, and are suggested for the treatment of osteoarthritis (OA). Here, we investigated whether intra-articular injection of xenogeneic human adipose-derived mesenchymal progenitor cells (haMPCs) promoted articular cartilage repair in rabbit OA model and engrafted into rabbit articular cartilage. The haMPCs were cultured in vitro, and phenotypes and differentiation characteristics of cells were evaluated. OA was induced surgically by anterior cruciate ligament transection (ACLT) and medical meniscectomy of knee joints. At six weeks following surgery, hyaluronic acid (HA) or haMPCs was injected into the knee joints, the contralateral knee served as normal control. All animals were sacrificed at the 16th week post-surgery. Assessments were carried out by macroscopic examination, hematoxylin/eosin (HE) and Safranin-O/Fast green stainings and immunohistochemistry. The data showed that haMPC treatment promoted cartilage repair. Signals of human mitochondrial can be directly detected in haMPC treated cartilage. The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo. These results suggest that intra-articular injection of haMPCs promotes regeneration of articular cartilage in rabbit OA model, and support the notion that MPCs are transplantable between HLA-incompatible individuals.

No MeSH data available.


Related in: MedlinePlus

Differentiation of human adipose-derived mesenchymal progenitor cells (haMPCs). Multipotent differentiation of haMPCs showed positive staining toward chondrogenesis (Alcian Blue), osteogenesis (Alizarin Red) and adipogenesis (Oil Red O). Scale bars = 50 μm.
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ijms-16-12076-f002: Differentiation of human adipose-derived mesenchymal progenitor cells (haMPCs). Multipotent differentiation of haMPCs showed positive staining toward chondrogenesis (Alcian Blue), osteogenesis (Alizarin Red) and adipogenesis (Oil Red O). Scale bars = 50 μm.

Mentions: MPCs are defined retrospectively by characteristics in vitro, including a combination of phenotypic markers and multipotential differentiation functional properties [21]. To begin our study of the effects of haMPCs on the OA model, we first examined the characteristics of haMPCs. haMPCs were isolated, expanded and characterized by flow cytometry. The results showed that haMPCs were positive for CD90, CD73, CD29, CD49d and HLA-I, while cells were negative for HLA-DR, Actin, CD14, CD45, CD34 (Figure 1). In addition, chondrogenesis of expanded haMPCs was confirmed by Alcian Blue staining, osteogenesis as determined by Alizarin Red staining and adipogenesis as assayed by Oil Red O staining (Figure 2). These results demonstrate that the cells were confirmed to the characterization of AD-MPCs.


Human adipose-derived mesenchymal progenitor cells engraft into rabbit articular cartilage.

Wang W, He N, Feng C, Liu V, Zhang L, Wang F, He J, Zhu T, Wang S, Qiao W, Li S, Zhou G, Zhang L, Dai C, Cao W - Int J Mol Sci (2015)

Differentiation of human adipose-derived mesenchymal progenitor cells (haMPCs). Multipotent differentiation of haMPCs showed positive staining toward chondrogenesis (Alcian Blue), osteogenesis (Alizarin Red) and adipogenesis (Oil Red O). Scale bars = 50 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490430&req=5

ijms-16-12076-f002: Differentiation of human adipose-derived mesenchymal progenitor cells (haMPCs). Multipotent differentiation of haMPCs showed positive staining toward chondrogenesis (Alcian Blue), osteogenesis (Alizarin Red) and adipogenesis (Oil Red O). Scale bars = 50 μm.
Mentions: MPCs are defined retrospectively by characteristics in vitro, including a combination of phenotypic markers and multipotential differentiation functional properties [21]. To begin our study of the effects of haMPCs on the OA model, we first examined the characteristics of haMPCs. haMPCs were isolated, expanded and characterized by flow cytometry. The results showed that haMPCs were positive for CD90, CD73, CD29, CD49d and HLA-I, while cells were negative for HLA-DR, Actin, CD14, CD45, CD34 (Figure 1). In addition, chondrogenesis of expanded haMPCs was confirmed by Alcian Blue staining, osteogenesis as determined by Alizarin Red staining and adipogenesis as assayed by Oil Red O staining (Figure 2). These results demonstrate that the cells were confirmed to the characterization of AD-MPCs.

Bottom Line: The data showed that haMPC treatment promoted cartilage repair.Signals of human mitochondrial can be directly detected in haMPC treated cartilage.The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo.

View Article: PubMed Central - PubMed

Affiliation: Cellular Biomedicine Group, Palo Alto, CA 94301, USA. maxwell.wang@cellbiomedgroup.com.

ABSTRACT
Mesenchymal stem cells (MSCs) are known to have the potential for articular cartilage regeneration, and are suggested for the treatment of osteoarthritis (OA). Here, we investigated whether intra-articular injection of xenogeneic human adipose-derived mesenchymal progenitor cells (haMPCs) promoted articular cartilage repair in rabbit OA model and engrafted into rabbit articular cartilage. The haMPCs were cultured in vitro, and phenotypes and differentiation characteristics of cells were evaluated. OA was induced surgically by anterior cruciate ligament transection (ACLT) and medical meniscectomy of knee joints. At six weeks following surgery, hyaluronic acid (HA) or haMPCs was injected into the knee joints, the contralateral knee served as normal control. All animals were sacrificed at the 16th week post-surgery. Assessments were carried out by macroscopic examination, hematoxylin/eosin (HE) and Safranin-O/Fast green stainings and immunohistochemistry. The data showed that haMPC treatment promoted cartilage repair. Signals of human mitochondrial can be directly detected in haMPC treated cartilage. The haMPCs expressed human leukocyte antigen I (HLA-I) but not HLA-II-DR in vivo. These results suggest that intra-articular injection of haMPCs promotes regeneration of articular cartilage in rabbit OA model, and support the notion that MPCs are transplantable between HLA-incompatible individuals.

No MeSH data available.


Related in: MedlinePlus