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High-throughput screening for a moderately halophilic phenol-degrading strain and its salt tolerance response.

Lu ZY, Guo XJ, Li H, Huang ZZ, Lin KF, Liu YD - Int J Mol Sci (2015)

Bottom Line: Bacterial enrichments were cultivated in 48 deep well microplates instead of shake flasks or tubes.Measurement of phenol concentrations was performed in 96-well microplates instead of using the conventional spectrophotometric method or high-performance liquid chromatography (HPLC).PCR detection of the functional genes suggested that the largest subunit of multicomponent phenol hydroxylase (LmPH) and catechol 1,2-dioxygenase (C12O) were active in the phenol degradation process.

View Article: PubMed Central - PubMed

Affiliation: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China. lzy1009a@163.com.

ABSTRACT
A high-throughput screening system for moderately halophilic phenol-degrading bacteria from various habitats was developed to replace the conventional strain screening owing to its high efficiency. Bacterial enrichments were cultivated in 48 deep well microplates instead of shake flasks or tubes. Measurement of phenol concentrations was performed in 96-well microplates instead of using the conventional spectrophotometric method or high-performance liquid chromatography (HPLC). The high-throughput screening system was used to cultivate forty-three bacterial enrichments and gained a halophilic bacterial community E3 with the best phenol-degrading capability. Halomonas sp. strain 4-5 was isolated from the E3 community. Strain 4-5 was able to degrade more than 94% of the phenol (500 mg · L(-1) starting concentration) over a range of 3%-10% NaCl. Additionally, the strain accumulated the compatible solute, ectoine, with increasing salt concentrations. PCR detection of the functional genes suggested that the largest subunit of multicomponent phenol hydroxylase (LmPH) and catechol 1,2-dioxygenase (C12O) were active in the phenol degradation process.

No MeSH data available.


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Results of high-throughput screening for halophilic bacterial phenol-degrading communities.
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ijms-16-11834-f003: Results of high-throughput screening for halophilic bacterial phenol-degrading communities.

Mentions: The ability to degrade phenol by 43 halophilic bacterial communities (No. A1–H1) cultivated in 48 deep well microplates was investigated. The residual phenol concentration in the 48 deep well microplates was measured by the high-throughput phenol measurement. The results showed that, of the 43 halophilic bacterial communities enriched, 10 degraded phenol above 50% in the presence of 3%–10% (w/v) NaCl (Figure 3). They were C3, C4, D6, E3, E4, E5, E6, F1, G4 and G5, which came from Daqing saline-alkaline soil, Shanghai Old Port Landfill Factory waste and the Shanghai Sinopec Gaoqiao petrochemical factory. By comparing with the phenol degradation of three bacterial communities (E3, D2, A1) under various salt conditions, the results showed that the phenol removal efficiency was higher under low salt conditions than the high salt conditions (Figure 4). When the salinity reached 12%, the phenol removal was severely inhibited by the salinity. The community E3 had the best degradation ability over a wide range of salinities, which came from Shanghai Old Port Landfill Factory waste. Five pure strains were then isolated from the E3 community. Among them, strain 4-5 showed optimal phenol-degrading characteristics.


High-throughput screening for a moderately halophilic phenol-degrading strain and its salt tolerance response.

Lu ZY, Guo XJ, Li H, Huang ZZ, Lin KF, Liu YD - Int J Mol Sci (2015)

Results of high-throughput screening for halophilic bacterial phenol-degrading communities.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490417&req=5

ijms-16-11834-f003: Results of high-throughput screening for halophilic bacterial phenol-degrading communities.
Mentions: The ability to degrade phenol by 43 halophilic bacterial communities (No. A1–H1) cultivated in 48 deep well microplates was investigated. The residual phenol concentration in the 48 deep well microplates was measured by the high-throughput phenol measurement. The results showed that, of the 43 halophilic bacterial communities enriched, 10 degraded phenol above 50% in the presence of 3%–10% (w/v) NaCl (Figure 3). They were C3, C4, D6, E3, E4, E5, E6, F1, G4 and G5, which came from Daqing saline-alkaline soil, Shanghai Old Port Landfill Factory waste and the Shanghai Sinopec Gaoqiao petrochemical factory. By comparing with the phenol degradation of three bacterial communities (E3, D2, A1) under various salt conditions, the results showed that the phenol removal efficiency was higher under low salt conditions than the high salt conditions (Figure 4). When the salinity reached 12%, the phenol removal was severely inhibited by the salinity. The community E3 had the best degradation ability over a wide range of salinities, which came from Shanghai Old Port Landfill Factory waste. Five pure strains were then isolated from the E3 community. Among them, strain 4-5 showed optimal phenol-degrading characteristics.

Bottom Line: Bacterial enrichments were cultivated in 48 deep well microplates instead of shake flasks or tubes.Measurement of phenol concentrations was performed in 96-well microplates instead of using the conventional spectrophotometric method or high-performance liquid chromatography (HPLC).PCR detection of the functional genes suggested that the largest subunit of multicomponent phenol hydroxylase (LmPH) and catechol 1,2-dioxygenase (C12O) were active in the phenol degradation process.

View Article: PubMed Central - PubMed

Affiliation: State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China. lzy1009a@163.com.

ABSTRACT
A high-throughput screening system for moderately halophilic phenol-degrading bacteria from various habitats was developed to replace the conventional strain screening owing to its high efficiency. Bacterial enrichments were cultivated in 48 deep well microplates instead of shake flasks or tubes. Measurement of phenol concentrations was performed in 96-well microplates instead of using the conventional spectrophotometric method or high-performance liquid chromatography (HPLC). The high-throughput screening system was used to cultivate forty-three bacterial enrichments and gained a halophilic bacterial community E3 with the best phenol-degrading capability. Halomonas sp. strain 4-5 was isolated from the E3 community. Strain 4-5 was able to degrade more than 94% of the phenol (500 mg · L(-1) starting concentration) over a range of 3%-10% NaCl. Additionally, the strain accumulated the compatible solute, ectoine, with increasing salt concentrations. PCR detection of the functional genes suggested that the largest subunit of multicomponent phenol hydroxylase (LmPH) and catechol 1,2-dioxygenase (C12O) were active in the phenol degradation process.

No MeSH data available.


Related in: MedlinePlus