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CD24 tracks divergent pluripotent states in mouse and human cells.

Shakiba N, White CA, Lipsitz YY, Yachie-Kinoshita A, Tonge PD, Hussein SM, Puri MC, Elbaz J, Morrissey-Scoot J, Li M, Munoz J, Benevento M, Rogers IM, Hanna JH, Heck AJ, Wollscheid B, Nagy A, Zandstra PW - Nat Commun (2015)

Bottom Line: Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths.Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells.Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, Toronto, Ontario, Canada M5S 3E1.

ABSTRACT
Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 as a marker that tracks the emergence of reprogramming-responsive cells, while enabling the analysis and enrichment of transgene-dependent (F-class) and -independent (traditional) induced pluripotent stem cells (iPSCs) at later stages. Furthermore, CD24 can be used to delineate epiblast stem cells (EpiSCs) from embryonic stem cells (ESCs) in mouse pluripotent culture. Importantly, regulated CD24 expression is conserved in human pluripotent stem cells (PSCs), tracking the conversion of human ESCs to more naive-like PSC states. Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture.

No MeSH data available.


Related in: MedlinePlus

Summary of CD24 as a marker for three pluripotent states.(a) Overview of CD24 as a tool for tracking transgene induction in early reprogramming, demarcating divergent reprogramming to F-class and ESC-like iPSC states in late reprogramming, and identifying multiple pluripotent states in transgene-independent pluripotent culture. (b) Summary of approach for identifying mouse F-class iPSCs, ESC-like iPSCs and EpiSCs via CD24/SSEA1/CD40 staining as well as human primed and naive hESCs via CD24/Tra-1-60 staining.
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f5: Summary of CD24 as a marker for three pluripotent states.(a) Overview of CD24 as a tool for tracking transgene induction in early reprogramming, demarcating divergent reprogramming to F-class and ESC-like iPSC states in late reprogramming, and identifying multiple pluripotent states in transgene-independent pluripotent culture. (b) Summary of approach for identifying mouse F-class iPSCs, ESC-like iPSCs and EpiSCs via CD24/SSEA1/CD40 staining as well as human primed and naive hESCs via CD24/Tra-1-60 staining.

Mentions: Here we have identified CD24 as a novel cell surface marker expressed dynamically during reprogramming. CD24 serves both as a marker to demarcate transgene-dependent (F-class) and -independent (ESC-like) mouse iPSCs and for delineating naive-like and primed pluripotent states in both mouse and human (Fig. 5).


CD24 tracks divergent pluripotent states in mouse and human cells.

Shakiba N, White CA, Lipsitz YY, Yachie-Kinoshita A, Tonge PD, Hussein SM, Puri MC, Elbaz J, Morrissey-Scoot J, Li M, Munoz J, Benevento M, Rogers IM, Hanna JH, Heck AJ, Wollscheid B, Nagy A, Zandstra PW - Nat Commun (2015)

Summary of CD24 as a marker for three pluripotent states.(a) Overview of CD24 as a tool for tracking transgene induction in early reprogramming, demarcating divergent reprogramming to F-class and ESC-like iPSC states in late reprogramming, and identifying multiple pluripotent states in transgene-independent pluripotent culture. (b) Summary of approach for identifying mouse F-class iPSCs, ESC-like iPSCs and EpiSCs via CD24/SSEA1/CD40 staining as well as human primed and naive hESCs via CD24/Tra-1-60 staining.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490408&req=5

f5: Summary of CD24 as a marker for three pluripotent states.(a) Overview of CD24 as a tool for tracking transgene induction in early reprogramming, demarcating divergent reprogramming to F-class and ESC-like iPSC states in late reprogramming, and identifying multiple pluripotent states in transgene-independent pluripotent culture. (b) Summary of approach for identifying mouse F-class iPSCs, ESC-like iPSCs and EpiSCs via CD24/SSEA1/CD40 staining as well as human primed and naive hESCs via CD24/Tra-1-60 staining.
Mentions: Here we have identified CD24 as a novel cell surface marker expressed dynamically during reprogramming. CD24 serves both as a marker to demarcate transgene-dependent (F-class) and -independent (ESC-like) mouse iPSCs and for delineating naive-like and primed pluripotent states in both mouse and human (Fig. 5).

Bottom Line: Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths.Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells.Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto, Toronto, Ontario, Canada M5S 3E1.

ABSTRACT
Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 as a marker that tracks the emergence of reprogramming-responsive cells, while enabling the analysis and enrichment of transgene-dependent (F-class) and -independent (traditional) induced pluripotent stem cells (iPSCs) at later stages. Furthermore, CD24 can be used to delineate epiblast stem cells (EpiSCs) from embryonic stem cells (ESCs) in mouse pluripotent culture. Importantly, regulated CD24 expression is conserved in human pluripotent stem cells (PSCs), tracking the conversion of human ESCs to more naive-like PSC states. Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture.

No MeSH data available.


Related in: MedlinePlus