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Salicylic acid biosynthesis is enhanced and contributes to increased biotrophic pathogen resistance in Arabidopsis hybrids.

Yang L, Li B, Zheng XY, Li J, Yang M, Dong X, He G, An C, Deng XW - Nat Commun (2015)

Bottom Line: Heterosis, the phenotypic superiority of a hybrid over its parents, has been demonstrated for many traits in Arabidopsis thaliana, but its effect on defence remains largely unexplored.Here, we show that hybrids between some A. thaliana accessions show increased resistance to the biotrophic bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000.Comparisons of transcriptomes between these hybrids and their parents after inoculation reveal that several key salicylic acid (SA) biosynthesis genes are significantly upregulated in hybrids.

View Article: PubMed Central - PubMed

Affiliation: 1] Peking-Yale Joint Center for Plant Molecular Genetics and Agro-Biotechnology, State Key Laboratory of Protein and Plant Gene Research, The Peking-Tsinghua Center for Life Sciences, School of Advanced Agricultural Sciences and School of Life Sciences, Peking University, Beijing 100871, China [2] Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA.

ABSTRACT
Heterosis, the phenotypic superiority of a hybrid over its parents, has been demonstrated for many traits in Arabidopsis thaliana, but its effect on defence remains largely unexplored. Here, we show that hybrids between some A. thaliana accessions show increased resistance to the biotrophic bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000. Comparisons of transcriptomes between these hybrids and their parents after inoculation reveal that several key salicylic acid (SA) biosynthesis genes are significantly upregulated in hybrids. Moreover, SA levels are higher in hybrids than in either parent. Increased resistance to Pst DC3000 is significantly compromised in hybrids of pad4 mutants in which the SA biosynthesis pathway is blocked. Finally, increased histone H3 acetylation of key SA biosynthesis genes correlates with their upregulation in infected hybrids. Our data demonstrate that enhanced activation of SA biosynthesis in A. thaliana hybrids may contribute to their increased resistance to a biotrophic bacterial pathogen.

No MeSH data available.


Related in: MedlinePlus

Working model of enhanced SA biosynthesis in hybrids.SA biosynthesis is activated in Arabidopsis thaliana F1 hybrids and their parents in response to pathogen invasion as a defence strategy, but is not observed in the absence of pathogens. Genes involved in SA biosynthesis were expressed at higher levels in F1 hybrids, possibly due to altered histone modifications (such as increased H3Ac). As a consequence, higher levels of SA accumulated in the F1 hybrids, which we propose plays an important role in the increased resistance of hybrids to biotrophic bacterial pathogens.
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f6: Working model of enhanced SA biosynthesis in hybrids.SA biosynthesis is activated in Arabidopsis thaliana F1 hybrids and their parents in response to pathogen invasion as a defence strategy, but is not observed in the absence of pathogens. Genes involved in SA biosynthesis were expressed at higher levels in F1 hybrids, possibly due to altered histone modifications (such as increased H3Ac). As a consequence, higher levels of SA accumulated in the F1 hybrids, which we propose plays an important role in the increased resistance of hybrids to biotrophic bacterial pathogens.

Mentions: Our data demonstrate that, in response to pathogen invasion, SA biosynthesis was activated, which has not been observed in the absence of pathogens, in both F1 hybrids and their parents as a defence strategy. However, the key genes involved in SA biosynthesis were expressed at higher levels in the F1 hybrids than in their parents, possibly through regulation of histone modifications, such as H3 acetylation. Consequently, higher levels of SA, which plays an important role in resistance to biotrophic bacterial pathogens in A. thaliana, are produced in F1 hybrids (Fig. 6).


Salicylic acid biosynthesis is enhanced and contributes to increased biotrophic pathogen resistance in Arabidopsis hybrids.

Yang L, Li B, Zheng XY, Li J, Yang M, Dong X, He G, An C, Deng XW - Nat Commun (2015)

Working model of enhanced SA biosynthesis in hybrids.SA biosynthesis is activated in Arabidopsis thaliana F1 hybrids and their parents in response to pathogen invasion as a defence strategy, but is not observed in the absence of pathogens. Genes involved in SA biosynthesis were expressed at higher levels in F1 hybrids, possibly due to altered histone modifications (such as increased H3Ac). As a consequence, higher levels of SA accumulated in the F1 hybrids, which we propose plays an important role in the increased resistance of hybrids to biotrophic bacterial pathogens.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490401&req=5

f6: Working model of enhanced SA biosynthesis in hybrids.SA biosynthesis is activated in Arabidopsis thaliana F1 hybrids and their parents in response to pathogen invasion as a defence strategy, but is not observed in the absence of pathogens. Genes involved in SA biosynthesis were expressed at higher levels in F1 hybrids, possibly due to altered histone modifications (such as increased H3Ac). As a consequence, higher levels of SA accumulated in the F1 hybrids, which we propose plays an important role in the increased resistance of hybrids to biotrophic bacterial pathogens.
Mentions: Our data demonstrate that, in response to pathogen invasion, SA biosynthesis was activated, which has not been observed in the absence of pathogens, in both F1 hybrids and their parents as a defence strategy. However, the key genes involved in SA biosynthesis were expressed at higher levels in the F1 hybrids than in their parents, possibly through regulation of histone modifications, such as H3 acetylation. Consequently, higher levels of SA, which plays an important role in resistance to biotrophic bacterial pathogens in A. thaliana, are produced in F1 hybrids (Fig. 6).

Bottom Line: Heterosis, the phenotypic superiority of a hybrid over its parents, has been demonstrated for many traits in Arabidopsis thaliana, but its effect on defence remains largely unexplored.Here, we show that hybrids between some A. thaliana accessions show increased resistance to the biotrophic bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000.Comparisons of transcriptomes between these hybrids and their parents after inoculation reveal that several key salicylic acid (SA) biosynthesis genes are significantly upregulated in hybrids.

View Article: PubMed Central - PubMed

Affiliation: 1] Peking-Yale Joint Center for Plant Molecular Genetics and Agro-Biotechnology, State Key Laboratory of Protein and Plant Gene Research, The Peking-Tsinghua Center for Life Sciences, School of Advanced Agricultural Sciences and School of Life Sciences, Peking University, Beijing 100871, China [2] Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA.

ABSTRACT
Heterosis, the phenotypic superiority of a hybrid over its parents, has been demonstrated for many traits in Arabidopsis thaliana, but its effect on defence remains largely unexplored. Here, we show that hybrids between some A. thaliana accessions show increased resistance to the biotrophic bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000. Comparisons of transcriptomes between these hybrids and their parents after inoculation reveal that several key salicylic acid (SA) biosynthesis genes are significantly upregulated in hybrids. Moreover, SA levels are higher in hybrids than in either parent. Increased resistance to Pst DC3000 is significantly compromised in hybrids of pad4 mutants in which the SA biosynthesis pathway is blocked. Finally, increased histone H3 acetylation of key SA biosynthesis genes correlates with their upregulation in infected hybrids. Our data demonstrate that enhanced activation of SA biosynthesis in A. thaliana hybrids may contribute to their increased resistance to a biotrophic bacterial pathogen.

No MeSH data available.


Related in: MedlinePlus