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Vitamin D Receptor Poly(A) Microsatellite Polymorphism and 25-Hydroxyvitamin D Serum Levels: Association with Susceptibility to Breast Cancer.

Colagar AH, Firouzjah HM, Halalkhor S - J Breast Cancer (2015)

Bottom Line: The vitamin D levels of samples were determined by electrochemiluminescence.A larger increase in the risk for breast cancer was found in individuals with the L carrier genotype and lowered 25(OH)D levels.The results primarily suggest that VDR gene polymorphism in the poly(A) microsatellite is associated with 25(OH)D levels and that it can affect the breast cancer risk in the female population from northern Iran.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Cell Biology, University of Mazandaran Faculty of Basic Sciences, Mazandaran, Iran.

ABSTRACT

Purpose: According to previous studies, vitamin D exhibits protective effects against breast cancer via the vitamin D receptor (VDR). There is growing evidence that breast cancer incidence is associated with various polymorphisms of the VDR gene. This study investigates the association of VDR poly(A) microsatellite variants with 25-hydroxyvitamin D (25(OH)D) serum levels and breast cancer risk.

Methods: Polymorphism analysis was performed on a total of 261 blood samples, which were collected from 134 women with breast cancer and 127 controls. Single strand conformation polymorphism was assessed by polymerase chain reaction in combination with sequencing to detect poly(A) lengths for each sample. The vitamin D levels of samples were determined by electrochemiluminescence.

Results: The poly(A) variant L allele frequency was significantly higher in cancer patients than in controls (odds ratio [OR], 1.73; 95% confidence interval [CI], 1.16-2.57; p=0.006). Thus, carriers of the L allele (LS and LL genotypes) have a higher risk for breast cancer (OR, 1.86; 95% CI, 1.13-3.05; p=0.013). A larger increase in the risk for breast cancer was found in individuals with the L carrier genotype and lowered 25(OH)D levels.

Conclusion: The results primarily suggest that VDR gene polymorphism in the poly(A) microsatellite is associated with 25(OH)D levels and that it can affect the breast cancer risk in the female population from northern Iran.

No MeSH data available.


Related in: MedlinePlus

Deoxyribonucleic acid electropherogram analysis of poly(A) repeat polymorphism in the 3'-untranslated region of vitamin D receptor gene regions: S and L alleles with poly(A)-microsatellite A-repeats 14-16 and 19-21, respectively.
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Figure 4: Deoxyribonucleic acid electropherogram analysis of poly(A) repeat polymorphism in the 3'-untranslated region of vitamin D receptor gene regions: S and L alleles with poly(A)-microsatellite A-repeats 14-16 and 19-21, respectively.

Mentions: After categorizing samples by SSCP, some homozygote samples were randomly selected from each group and prepared for direct sequencing. For sequencing of heterozygote samples, each allele was extracted from polyacrylamide gel electrophoresis (PAGE) by crush and soak method [21] and then amplified by PCR. Direct DNA sequencing of the purified PCR product was performed on an ABI 3730XL DNA Analyzer (Bioneer, Daejeon, Korea) (Figure 4).


Vitamin D Receptor Poly(A) Microsatellite Polymorphism and 25-Hydroxyvitamin D Serum Levels: Association with Susceptibility to Breast Cancer.

Colagar AH, Firouzjah HM, Halalkhor S - J Breast Cancer (2015)

Deoxyribonucleic acid electropherogram analysis of poly(A) repeat polymorphism in the 3'-untranslated region of vitamin D receptor gene regions: S and L alleles with poly(A)-microsatellite A-repeats 14-16 and 19-21, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490260&req=5

Figure 4: Deoxyribonucleic acid electropherogram analysis of poly(A) repeat polymorphism in the 3'-untranslated region of vitamin D receptor gene regions: S and L alleles with poly(A)-microsatellite A-repeats 14-16 and 19-21, respectively.
Mentions: After categorizing samples by SSCP, some homozygote samples were randomly selected from each group and prepared for direct sequencing. For sequencing of heterozygote samples, each allele was extracted from polyacrylamide gel electrophoresis (PAGE) by crush and soak method [21] and then amplified by PCR. Direct DNA sequencing of the purified PCR product was performed on an ABI 3730XL DNA Analyzer (Bioneer, Daejeon, Korea) (Figure 4).

Bottom Line: The vitamin D levels of samples were determined by electrochemiluminescence.A larger increase in the risk for breast cancer was found in individuals with the L carrier genotype and lowered 25(OH)D levels.The results primarily suggest that VDR gene polymorphism in the poly(A) microsatellite is associated with 25(OH)D levels and that it can affect the breast cancer risk in the female population from northern Iran.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Cell Biology, University of Mazandaran Faculty of Basic Sciences, Mazandaran, Iran.

ABSTRACT

Purpose: According to previous studies, vitamin D exhibits protective effects against breast cancer via the vitamin D receptor (VDR). There is growing evidence that breast cancer incidence is associated with various polymorphisms of the VDR gene. This study investigates the association of VDR poly(A) microsatellite variants with 25-hydroxyvitamin D (25(OH)D) serum levels and breast cancer risk.

Methods: Polymorphism analysis was performed on a total of 261 blood samples, which were collected from 134 women with breast cancer and 127 controls. Single strand conformation polymorphism was assessed by polymerase chain reaction in combination with sequencing to detect poly(A) lengths for each sample. The vitamin D levels of samples were determined by electrochemiluminescence.

Results: The poly(A) variant L allele frequency was significantly higher in cancer patients than in controls (odds ratio [OR], 1.73; 95% confidence interval [CI], 1.16-2.57; p=0.006). Thus, carriers of the L allele (LS and LL genotypes) have a higher risk for breast cancer (OR, 1.86; 95% CI, 1.13-3.05; p=0.013). A larger increase in the risk for breast cancer was found in individuals with the L carrier genotype and lowered 25(OH)D levels.

Conclusion: The results primarily suggest that VDR gene polymorphism in the poly(A) microsatellite is associated with 25(OH)D levels and that it can affect the breast cancer risk in the female population from northern Iran.

No MeSH data available.


Related in: MedlinePlus