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Vitamin D Receptor Poly(A) Microsatellite Polymorphism and 25-Hydroxyvitamin D Serum Levels: Association with Susceptibility to Breast Cancer.

Colagar AH, Firouzjah HM, Halalkhor S - J Breast Cancer (2015)

Bottom Line: The vitamin D levels of samples were determined by electrochemiluminescence.A larger increase in the risk for breast cancer was found in individuals with the L carrier genotype and lowered 25(OH)D levels.The results primarily suggest that VDR gene polymorphism in the poly(A) microsatellite is associated with 25(OH)D levels and that it can affect the breast cancer risk in the female population from northern Iran.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Cell Biology, University of Mazandaran Faculty of Basic Sciences, Mazandaran, Iran.

ABSTRACT

Purpose: According to previous studies, vitamin D exhibits protective effects against breast cancer via the vitamin D receptor (VDR). There is growing evidence that breast cancer incidence is associated with various polymorphisms of the VDR gene. This study investigates the association of VDR poly(A) microsatellite variants with 25-hydroxyvitamin D (25(OH)D) serum levels and breast cancer risk.

Methods: Polymorphism analysis was performed on a total of 261 blood samples, which were collected from 134 women with breast cancer and 127 controls. Single strand conformation polymorphism was assessed by polymerase chain reaction in combination with sequencing to detect poly(A) lengths for each sample. The vitamin D levels of samples were determined by electrochemiluminescence.

Results: The poly(A) variant L allele frequency was significantly higher in cancer patients than in controls (odds ratio [OR], 1.73; 95% confidence interval [CI], 1.16-2.57; p=0.006). Thus, carriers of the L allele (LS and LL genotypes) have a higher risk for breast cancer (OR, 1.86; 95% CI, 1.13-3.05; p=0.013). A larger increase in the risk for breast cancer was found in individuals with the L carrier genotype and lowered 25(OH)D levels.

Conclusion: The results primarily suggest that VDR gene polymorphism in the poly(A) microsatellite is associated with 25(OH)D levels and that it can affect the breast cancer risk in the female population from northern Iran.

No MeSH data available.


Related in: MedlinePlus

Single-strand conformation polymorphism (SSCP) results of the polymerase chain reaction (PCR) product in denaturing polyacrylamide gel electrophoresis, visualized by silver nitrate staining: (A) PCR products of the SS genotype with ~236-238 bp and LL genotype with ~241-243 bp in SSCP gel; (B) Schematic of the adenine numeric repeat shown by number in single strand oligonucleotide bands or different alleles.
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Figure 3: Single-strand conformation polymorphism (SSCP) results of the polymerase chain reaction (PCR) product in denaturing polyacrylamide gel electrophoresis, visualized by silver nitrate staining: (A) PCR products of the SS genotype with ~236-238 bp and LL genotype with ~241-243 bp in SSCP gel; (B) Schematic of the adenine numeric repeat shown by number in single strand oligonucleotide bands or different alleles.

Mentions: Detection of poly(A) length in PCR products was performed by SSCP. For this purpose, 3 µL PCR product was mixed with 12 µL SSCP dye (0.05% bromophenol blue, 0.05% xylene cyanol, 95% formamide, 20 mM EDTA). Before loading, the samples were denatured at 95℃ for 5 minutes and then placed on ice for 3 minutes. The samples were loaded on an 8% polyacrylamide gel and visualized by AgNO3 staining (Figure 3) [20].


Vitamin D Receptor Poly(A) Microsatellite Polymorphism and 25-Hydroxyvitamin D Serum Levels: Association with Susceptibility to Breast Cancer.

Colagar AH, Firouzjah HM, Halalkhor S - J Breast Cancer (2015)

Single-strand conformation polymorphism (SSCP) results of the polymerase chain reaction (PCR) product in denaturing polyacrylamide gel electrophoresis, visualized by silver nitrate staining: (A) PCR products of the SS genotype with ~236-238 bp and LL genotype with ~241-243 bp in SSCP gel; (B) Schematic of the adenine numeric repeat shown by number in single strand oligonucleotide bands or different alleles.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490260&req=5

Figure 3: Single-strand conformation polymorphism (SSCP) results of the polymerase chain reaction (PCR) product in denaturing polyacrylamide gel electrophoresis, visualized by silver nitrate staining: (A) PCR products of the SS genotype with ~236-238 bp and LL genotype with ~241-243 bp in SSCP gel; (B) Schematic of the adenine numeric repeat shown by number in single strand oligonucleotide bands or different alleles.
Mentions: Detection of poly(A) length in PCR products was performed by SSCP. For this purpose, 3 µL PCR product was mixed with 12 µL SSCP dye (0.05% bromophenol blue, 0.05% xylene cyanol, 95% formamide, 20 mM EDTA). Before loading, the samples were denatured at 95℃ for 5 minutes and then placed on ice for 3 minutes. The samples were loaded on an 8% polyacrylamide gel and visualized by AgNO3 staining (Figure 3) [20].

Bottom Line: The vitamin D levels of samples were determined by electrochemiluminescence.A larger increase in the risk for breast cancer was found in individuals with the L carrier genotype and lowered 25(OH)D levels.The results primarily suggest that VDR gene polymorphism in the poly(A) microsatellite is associated with 25(OH)D levels and that it can affect the breast cancer risk in the female population from northern Iran.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular and Cell Biology, University of Mazandaran Faculty of Basic Sciences, Mazandaran, Iran.

ABSTRACT

Purpose: According to previous studies, vitamin D exhibits protective effects against breast cancer via the vitamin D receptor (VDR). There is growing evidence that breast cancer incidence is associated with various polymorphisms of the VDR gene. This study investigates the association of VDR poly(A) microsatellite variants with 25-hydroxyvitamin D (25(OH)D) serum levels and breast cancer risk.

Methods: Polymorphism analysis was performed on a total of 261 blood samples, which were collected from 134 women with breast cancer and 127 controls. Single strand conformation polymorphism was assessed by polymerase chain reaction in combination with sequencing to detect poly(A) lengths for each sample. The vitamin D levels of samples were determined by electrochemiluminescence.

Results: The poly(A) variant L allele frequency was significantly higher in cancer patients than in controls (odds ratio [OR], 1.73; 95% confidence interval [CI], 1.16-2.57; p=0.006). Thus, carriers of the L allele (LS and LL genotypes) have a higher risk for breast cancer (OR, 1.86; 95% CI, 1.13-3.05; p=0.013). A larger increase in the risk for breast cancer was found in individuals with the L carrier genotype and lowered 25(OH)D levels.

Conclusion: The results primarily suggest that VDR gene polymorphism in the poly(A) microsatellite is associated with 25(OH)D levels and that it can affect the breast cancer risk in the female population from northern Iran.

No MeSH data available.


Related in: MedlinePlus