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A new leafminer on grapevine and Rhoicissus (Vitaceae) in South Africa within an expanded generic concept of Holocacista (Insecta, Lepidoptera, Heliozelidae).

van Nieukerken EJ, Geertsema H - Zookeys (2015)

Bottom Line: A grapevine leafminer found recently in table grape orchards and vineyards in the Paarl region (Western Cape, South Africa) is described as Holocacistacapensis sp. n.It has also been found on native Rhoicissusdigitata and bred on that species in the laboratory.Holocacistacapensis has been found on Vitisvinifera both in Gauteng and Western Cape, the earliest record being from 1950 in Pretoria.

View Article: PubMed Central - HTML - PubMed

Affiliation: Naturalis Biodiversity Center, PO Box 9557, NL-2300 RA Leiden, The Netherlands.

ABSTRACT
A grapevine leafminer found recently in table grape orchards and vineyards in the Paarl region (Western Cape, South Africa) is described as Holocacistacapensis sp. n. It has also been found on native Rhoicissusdigitata and bred on that species in the laboratory. It is closely related to Holocacistasalutans (Meyrick, 1921), comb. n. (from Antispila), described from Durban in KwaZulu-Natal, but widespread in southern Africa and a native leafminer of various Vitaceae: Rhoicissustomentosa, Rhoicissusdigitata, Rhoicissustridentata and Cissuscornifolia. Holocacistacapensis has been found on Vitisvinifera both in Gauteng and Western Cape, the earliest record being from 1950 in Pretoria. The initial host shift from native Vitaceae to Vitis must have occurred much earlier. The species is sometimes present in high densities, but hitherto no sizeable damage to the crops has been noted. The genus Holocacista Walsingham & Durrant, 1909, previously known from the single European grapevine leafminer Holocacistarivillei (Stainton, 1855), is expanded and redescribed and for the first time reported from Africa, East and South-East Asia and Australia. It comprises seven named species and at least 15 unnamed species. The following species are also recombined with Holocacista: transferred from Antispilina: South-African Holocacistavarii (Mey, 2011), comb. n., feeding on Pelargonium, transferred from Antispila: the Indian species Holocacistamicrarcha (Meyrick, 1926), comb. n. and Holocacistapariodelta (Meyrick, 1929), comb. n., both feeding on Lanneacoromandelica, and Holocacistaselastis (Meyrick, 1926), comb. n. on Psychotriadalzelii. We also remove the following from Antispila: Heliozelaanna (Fletcher, 1920), comb. n. and Heliozelaargyrozona (Meyrick, 1918), comb. n., whereas the following Indian Vitaceae feeding species are confirmed to belong in Antispila s. str.: Antispilaargostoma Meyrick, 1916 and Antispilaaristarcha Meyrick, 1916. Holocacistasalutans and Holocacistavarii are redescribed and diagnosed against Holocacistacapensis and other South African Heliozelidae. DNA barcodes are provided for 13 species of Holocacista.

No MeSH data available.


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Neighbor Joining Tree, based on DNA barcodes of Holocacista species and other Heliozelidae.
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Figure 21: Neighbor Joining Tree, based on DNA barcodes of Holocacista species and other Heliozelidae.

Mentions: We sequenced eight specimens of Holocacistacapensis and compared those with 24 sequences of other Holocacista species and several other heliozelid barcodes, sequenced for previous studies (van Nieukerken et al. 2012b; Bernardo et al. 2015). The NJ tree is presented in Fig. 117. The barcodes of Holocacistacapensis group clearly together and can only be divided in three haplotypes, two from the Paarl region, which just differ in a single substitution on position 59 (C or T), whereas the single sequenced specimen from Gauteng has a difference of 1.4% (9 nucleotides). The nearest neighbours are a specimen of Holocacistavarii and a specimen of Holocacistarivillei, each with a distance of 11.2%. The three sequenced larvae from Rhoicissus represent two barcode clusters: respectively the two larvae from Rhoicissustridentata in North West province (12.0–12.2% distance from Holocacistacapensis), and the single sequence of a larva from Rhoicissustomentosa in Western Cape (11.9% distance). We failed in amplifying DNA from collection specimens of South African Heliozelidae stored in TMSA, also when using primers for smaller parts of the CO1 gene.


A new leafminer on grapevine and Rhoicissus (Vitaceae) in South Africa within an expanded generic concept of Holocacista (Insecta, Lepidoptera, Heliozelidae).

van Nieukerken EJ, Geertsema H - Zookeys (2015)

Neighbor Joining Tree, based on DNA barcodes of Holocacista species and other Heliozelidae.
© Copyright Policy - creative-commons-attribution
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4490219&req=5

Figure 21: Neighbor Joining Tree, based on DNA barcodes of Holocacista species and other Heliozelidae.
Mentions: We sequenced eight specimens of Holocacistacapensis and compared those with 24 sequences of other Holocacista species and several other heliozelid barcodes, sequenced for previous studies (van Nieukerken et al. 2012b; Bernardo et al. 2015). The NJ tree is presented in Fig. 117. The barcodes of Holocacistacapensis group clearly together and can only be divided in three haplotypes, two from the Paarl region, which just differ in a single substitution on position 59 (C or T), whereas the single sequenced specimen from Gauteng has a difference of 1.4% (9 nucleotides). The nearest neighbours are a specimen of Holocacistavarii and a specimen of Holocacistarivillei, each with a distance of 11.2%. The three sequenced larvae from Rhoicissus represent two barcode clusters: respectively the two larvae from Rhoicissustridentata in North West province (12.0–12.2% distance from Holocacistacapensis), and the single sequence of a larva from Rhoicissustomentosa in Western Cape (11.9% distance). We failed in amplifying DNA from collection specimens of South African Heliozelidae stored in TMSA, also when using primers for smaller parts of the CO1 gene.

Bottom Line: A grapevine leafminer found recently in table grape orchards and vineyards in the Paarl region (Western Cape, South Africa) is described as Holocacistacapensis sp. n.It has also been found on native Rhoicissusdigitata and bred on that species in the laboratory.Holocacistacapensis has been found on Vitisvinifera both in Gauteng and Western Cape, the earliest record being from 1950 in Pretoria.

View Article: PubMed Central - HTML - PubMed

Affiliation: Naturalis Biodiversity Center, PO Box 9557, NL-2300 RA Leiden, The Netherlands.

ABSTRACT
A grapevine leafminer found recently in table grape orchards and vineyards in the Paarl region (Western Cape, South Africa) is described as Holocacistacapensis sp. n. It has also been found on native Rhoicissusdigitata and bred on that species in the laboratory. It is closely related to Holocacistasalutans (Meyrick, 1921), comb. n. (from Antispila), described from Durban in KwaZulu-Natal, but widespread in southern Africa and a native leafminer of various Vitaceae: Rhoicissustomentosa, Rhoicissusdigitata, Rhoicissustridentata and Cissuscornifolia. Holocacistacapensis has been found on Vitisvinifera both in Gauteng and Western Cape, the earliest record being from 1950 in Pretoria. The initial host shift from native Vitaceae to Vitis must have occurred much earlier. The species is sometimes present in high densities, but hitherto no sizeable damage to the crops has been noted. The genus Holocacista Walsingham & Durrant, 1909, previously known from the single European grapevine leafminer Holocacistarivillei (Stainton, 1855), is expanded and redescribed and for the first time reported from Africa, East and South-East Asia and Australia. It comprises seven named species and at least 15 unnamed species. The following species are also recombined with Holocacista: transferred from Antispilina: South-African Holocacistavarii (Mey, 2011), comb. n., feeding on Pelargonium, transferred from Antispila: the Indian species Holocacistamicrarcha (Meyrick, 1926), comb. n. and Holocacistapariodelta (Meyrick, 1929), comb. n., both feeding on Lanneacoromandelica, and Holocacistaselastis (Meyrick, 1926), comb. n. on Psychotriadalzelii. We also remove the following from Antispila: Heliozelaanna (Fletcher, 1920), comb. n. and Heliozelaargyrozona (Meyrick, 1918), comb. n., whereas the following Indian Vitaceae feeding species are confirmed to belong in Antispila s. str.: Antispilaargostoma Meyrick, 1916 and Antispilaaristarcha Meyrick, 1916. Holocacistasalutans and Holocacistavarii are redescribed and diagnosed against Holocacistacapensis and other South African Heliozelidae. DNA barcodes are provided for 13 species of Holocacista.

No MeSH data available.


Related in: MedlinePlus