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Tumor necrosis factor alpha inhibits ovulation and induces granulosa cell death in rat ovaries.

Yamamoto Y, Kuwahara A, Taniguchi Y, Yamasaki M, Tanaka Y, Mukai Y, Yamashita M, Matsuzaki T, Yasui T, Irahara M - Reprod. Med. Biol. (2014)

Bottom Line: TNFα significantly decreased the number of released oocytes, and many unruptured follicles were observed.Furthermore, the expression of LC3 wwas significantly higher than that of the control, and autophagosomes were observed in the cytoplasm.Our data indicated that TNFα is an important mediator of ovulation in terms of decreasing the number of released oocytes and inducing granulosa cell death of unruptured follicles via apoptosis and autophagy for remodeling ovarian tissues.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, The University of Tokushima, Institute for Health Biosciences, 3-18-15 Kuramoto-cho, Tokushima, 770-8503 Japan.

ABSTRACT

Purpose: We evaluated the role of tumor necrosis factor alpha (TNFα) in rat ovulation and granulosa cell death of ovarian follicles during the periovulatory stage.

Methods: Immature rats primed with pregnant mare serum gonadotropin were injected intraperitoneally with human chorionic gonadotropin (hCG), and TNFα was injected into the bursa 48 h later. The total number of released oocytes was counted. Apoptosis was measured with terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and the expression of cleaved caspase 3 and Bax/Bcl-2. Autophagy was assessed by the expression of light chain protein 3 (LC3) and autophagosomes under transmission electron microscopy.

Results: TNFα significantly decreased the number of released oocytes, and many unruptured follicles were observed. TUNEL analysis revealed a larger number of apoptotic cells, and the cleaved caspase 3 and Bax/Bcl-2 increased more than that of the control 12 h after hCG administration. Furthermore, the expression of LC3 wwas significantly higher than that of the control, and autophagosomes were observed in the cytoplasm.

Conclusions: Our data indicated that TNFα is an important mediator of ovulation in terms of decreasing the number of released oocytes and inducing granulosa cell death of unruptured follicles via apoptosis and autophagy for remodeling ovarian tissues.

No MeSH data available.


Related in: MedlinePlus

Number of released oocytes and histological examination of the ovaries. The number of oocytes is shown as the mean ±SD (a). A significant difference (p < 0.01) was found between groups (n = 4). Many unluteinized, unruptured follicles were observed 24 h after hCG treatment in the TNFα group (arrows), though many corpus luteinized follicles were observed in the control group (b)
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Fig1: Number of released oocytes and histological examination of the ovaries. The number of oocytes is shown as the mean ±SD (a). A significant difference (p < 0.01) was found between groups (n = 4). Many unluteinized, unruptured follicles were observed 24 h after hCG treatment in the TNFα group (arrows), though many corpus luteinized follicles were observed in the control group (b)

Mentions: Twenty-four hours after hCG administration, the total number of released oocytes decreased in the TNFα group concentration-dependency (control: 31.3 ±6.2 vs. TNFα50 ng: 18.8 ±3.9 vs. TNFα100 ng: 7.0 ±3.7). Especially, TNFα100 ng was significantly lower than that in the control group (p < 0.01; Fig. 1a) (n = 4).Fig. 1


Tumor necrosis factor alpha inhibits ovulation and induces granulosa cell death in rat ovaries.

Yamamoto Y, Kuwahara A, Taniguchi Y, Yamasaki M, Tanaka Y, Mukai Y, Yamashita M, Matsuzaki T, Yasui T, Irahara M - Reprod. Med. Biol. (2014)

Number of released oocytes and histological examination of the ovaries. The number of oocytes is shown as the mean ±SD (a). A significant difference (p < 0.01) was found between groups (n = 4). Many unluteinized, unruptured follicles were observed 24 h after hCG treatment in the TNFα group (arrows), though many corpus luteinized follicles were observed in the control group (b)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4490172&req=5

Fig1: Number of released oocytes and histological examination of the ovaries. The number of oocytes is shown as the mean ±SD (a). A significant difference (p < 0.01) was found between groups (n = 4). Many unluteinized, unruptured follicles were observed 24 h after hCG treatment in the TNFα group (arrows), though many corpus luteinized follicles were observed in the control group (b)
Mentions: Twenty-four hours after hCG administration, the total number of released oocytes decreased in the TNFα group concentration-dependency (control: 31.3 ±6.2 vs. TNFα50 ng: 18.8 ±3.9 vs. TNFα100 ng: 7.0 ±3.7). Especially, TNFα100 ng was significantly lower than that in the control group (p < 0.01; Fig. 1a) (n = 4).Fig. 1

Bottom Line: TNFα significantly decreased the number of released oocytes, and many unruptured follicles were observed.Furthermore, the expression of LC3 wwas significantly higher than that of the control, and autophagosomes were observed in the cytoplasm.Our data indicated that TNFα is an important mediator of ovulation in terms of decreasing the number of released oocytes and inducing granulosa cell death of unruptured follicles via apoptosis and autophagy for remodeling ovarian tissues.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, The University of Tokushima, Institute for Health Biosciences, 3-18-15 Kuramoto-cho, Tokushima, 770-8503 Japan.

ABSTRACT

Purpose: We evaluated the role of tumor necrosis factor alpha (TNFα) in rat ovulation and granulosa cell death of ovarian follicles during the periovulatory stage.

Methods: Immature rats primed with pregnant mare serum gonadotropin were injected intraperitoneally with human chorionic gonadotropin (hCG), and TNFα was injected into the bursa 48 h later. The total number of released oocytes was counted. Apoptosis was measured with terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and the expression of cleaved caspase 3 and Bax/Bcl-2. Autophagy was assessed by the expression of light chain protein 3 (LC3) and autophagosomes under transmission electron microscopy.

Results: TNFα significantly decreased the number of released oocytes, and many unruptured follicles were observed. TUNEL analysis revealed a larger number of apoptotic cells, and the cleaved caspase 3 and Bax/Bcl-2 increased more than that of the control 12 h after hCG administration. Furthermore, the expression of LC3 wwas significantly higher than that of the control, and autophagosomes were observed in the cytoplasm.

Conclusions: Our data indicated that TNFα is an important mediator of ovulation in terms of decreasing the number of released oocytes and inducing granulosa cell death of unruptured follicles via apoptosis and autophagy for remodeling ovarian tissues.

No MeSH data available.


Related in: MedlinePlus