Limits...
Antidiabetic, antioxidant, antihyperlipidemic effect of extract of Euryale ferox salisb. with enhanced histopathology of pancreas, liver and kidney in streptozotocin induced diabetic rats.

Ahmed D, Kumar V, Verma A, Shukla GS, Sharma M - Springerplus (2015)

Bottom Line: Biochemical investigations were carried out according to previously reported methods.Histopathological examinations were done accordingly.Activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH) were significantly increased (p < 0.001) among EFx treated rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences (SHIATS)-Deemed University, Allahabad, India.

ABSTRACT

Background: Ethanolic extract of Euryale ferox salisb. (EFx) may have an effect on the activity of hepatic antioxidant enzymes, glycemic control and lipid profile and histopathology of pancreas, liver and kidney of streptozotocin (STZ)-induced diabetic wistar rats.

Methods: Wistar albino rats were divided into eight groups viz. non-diabetic (normal control), diabetic control (STZ-induced), diabetic treated (infused with different doses of Euryale ferox. Salisb. ethanolic extract) and diabetic conventional treated (treated with Glibenclamide). Diabetes was induced by administering streptozotocin (60 mg/kg body weight) intraperitoneal (i.p). The ethanolic extract was supplemented in different doses through oral route. Biochemical investigations were carried out according to previously reported methods. Histopathological examinations were done accordingly.

Results: The EFx supplemented diabetic rats significantly (p < 0.001) decreased the blood glucose level in a dose dependent manner. Plasma insulin level was significantly increased in EFx treated rats. The hepatic gluconeogenic enzymes activities were restored to normal in EFx treated rats. Activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH) were significantly increased (p < 0.001) among EFx treated rats. Lipid profile was reinstated to nearly normal level among EFx treated rats. Histopathological investigations revealed that microscopic architecture of pancreatic, hepatic and renal cells improvised in EFx treated diabetic rats.

Conclusion: EFx supplement could improve the glycemic control as well as lipid profile in diabetic rats along with improvised antioxidant enzymes which has beneficial effect in preventing the diabetic complications by scavenging the free radicals in diabetic rats.

No MeSH data available.


Related in: MedlinePlus

Effect of Euryale ferox salisb seeds extract (EFx) on histological profile of pancreas in normal, STZ-induced diabetic untreated and STZ-induced diabetic treated wistar rats (original magnification ×10, DXIT 1200, Nikon, Japan). (1) NPAN: heamatoxylin and eosin (H/E) stained sections of pancreas of normal control rat portraying normal islet of langerhans shown by yellow arrows. (2) PSTZ: pancreatic section of streptozotocin induced diabetic rat showing no/destroyed islet of langerhans and beta cells depicted by red arrows. (3) PEFx-100: pancreatic section of STZ-induced diabetic rats treated with EFx at 100 mg/kg body weight showing small number of islet of langerhans (green arrows). (4) PEFx-200: section of pancreas of STZ-induced diabetic rats treated with EFx at 200 mg/kg body weight portraying increased number of islet of langerhans with small proportions of beta cells (green arrows). (5) PEFx-300: pancreas of diabetic rats treated with 300 mg/kg body weight. EFx depicting nearly normal islet of langerhans (green arrows). (6) PEFx-400: sections of pancreas of diabetic treated rats with 400 mg/kg body weight. EFx showing normal islet of langerhans with numerous beta cells (green arrows). (7) PGLIM: pancreatic section of diabetic rats treated with Glibenclamide showing normal pancreatic islet of langerhans with enhancement in the number of beta cells (dark yellow arrow).
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4489967&req=5

Fig9: Effect of Euryale ferox salisb seeds extract (EFx) on histological profile of pancreas in normal, STZ-induced diabetic untreated and STZ-induced diabetic treated wistar rats (original magnification ×10, DXIT 1200, Nikon, Japan). (1) NPAN: heamatoxylin and eosin (H/E) stained sections of pancreas of normal control rat portraying normal islet of langerhans shown by yellow arrows. (2) PSTZ: pancreatic section of streptozotocin induced diabetic rat showing no/destroyed islet of langerhans and beta cells depicted by red arrows. (3) PEFx-100: pancreatic section of STZ-induced diabetic rats treated with EFx at 100 mg/kg body weight showing small number of islet of langerhans (green arrows). (4) PEFx-200: section of pancreas of STZ-induced diabetic rats treated with EFx at 200 mg/kg body weight portraying increased number of islet of langerhans with small proportions of beta cells (green arrows). (5) PEFx-300: pancreas of diabetic rats treated with 300 mg/kg body weight. EFx depicting nearly normal islet of langerhans (green arrows). (6) PEFx-400: sections of pancreas of diabetic treated rats with 400 mg/kg body weight. EFx showing normal islet of langerhans with numerous beta cells (green arrows). (7) PGLIM: pancreatic section of diabetic rats treated with Glibenclamide showing normal pancreatic islet of langerhans with enhancement in the number of beta cells (dark yellow arrow).

Mentions: Normal control rats exhibited normal histological architecture. Many rounded normal proportions of islet of langerhans were found all around the pancreatic acini. Prominent nuclei with well arranged lobules with surrounding islet cells, were found among normal control rats (Figure 8). Groups received STZ, demonstrated cellular damage to the pancreatic acini and islets, which showed pancreatic β-cell damage and degeneration with asymmetrical vacuoles. EFx treated STZ induced-DM rats showed marked improvement of the cellular injure as (Figure 9), as evident from the partial restoration of islet cells, reduced β-cell damage, more symmetrical vacuoles and an increase in number of islet cells.Figure 8


Antidiabetic, antioxidant, antihyperlipidemic effect of extract of Euryale ferox salisb. with enhanced histopathology of pancreas, liver and kidney in streptozotocin induced diabetic rats.

Ahmed D, Kumar V, Verma A, Shukla GS, Sharma M - Springerplus (2015)

Effect of Euryale ferox salisb seeds extract (EFx) on histological profile of pancreas in normal, STZ-induced diabetic untreated and STZ-induced diabetic treated wistar rats (original magnification ×10, DXIT 1200, Nikon, Japan). (1) NPAN: heamatoxylin and eosin (H/E) stained sections of pancreas of normal control rat portraying normal islet of langerhans shown by yellow arrows. (2) PSTZ: pancreatic section of streptozotocin induced diabetic rat showing no/destroyed islet of langerhans and beta cells depicted by red arrows. (3) PEFx-100: pancreatic section of STZ-induced diabetic rats treated with EFx at 100 mg/kg body weight showing small number of islet of langerhans (green arrows). (4) PEFx-200: section of pancreas of STZ-induced diabetic rats treated with EFx at 200 mg/kg body weight portraying increased number of islet of langerhans with small proportions of beta cells (green arrows). (5) PEFx-300: pancreas of diabetic rats treated with 300 mg/kg body weight. EFx depicting nearly normal islet of langerhans (green arrows). (6) PEFx-400: sections of pancreas of diabetic treated rats with 400 mg/kg body weight. EFx showing normal islet of langerhans with numerous beta cells (green arrows). (7) PGLIM: pancreatic section of diabetic rats treated with Glibenclamide showing normal pancreatic islet of langerhans with enhancement in the number of beta cells (dark yellow arrow).
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4489967&req=5

Fig9: Effect of Euryale ferox salisb seeds extract (EFx) on histological profile of pancreas in normal, STZ-induced diabetic untreated and STZ-induced diabetic treated wistar rats (original magnification ×10, DXIT 1200, Nikon, Japan). (1) NPAN: heamatoxylin and eosin (H/E) stained sections of pancreas of normal control rat portraying normal islet of langerhans shown by yellow arrows. (2) PSTZ: pancreatic section of streptozotocin induced diabetic rat showing no/destroyed islet of langerhans and beta cells depicted by red arrows. (3) PEFx-100: pancreatic section of STZ-induced diabetic rats treated with EFx at 100 mg/kg body weight showing small number of islet of langerhans (green arrows). (4) PEFx-200: section of pancreas of STZ-induced diabetic rats treated with EFx at 200 mg/kg body weight portraying increased number of islet of langerhans with small proportions of beta cells (green arrows). (5) PEFx-300: pancreas of diabetic rats treated with 300 mg/kg body weight. EFx depicting nearly normal islet of langerhans (green arrows). (6) PEFx-400: sections of pancreas of diabetic treated rats with 400 mg/kg body weight. EFx showing normal islet of langerhans with numerous beta cells (green arrows). (7) PGLIM: pancreatic section of diabetic rats treated with Glibenclamide showing normal pancreatic islet of langerhans with enhancement in the number of beta cells (dark yellow arrow).
Mentions: Normal control rats exhibited normal histological architecture. Many rounded normal proportions of islet of langerhans were found all around the pancreatic acini. Prominent nuclei with well arranged lobules with surrounding islet cells, were found among normal control rats (Figure 8). Groups received STZ, demonstrated cellular damage to the pancreatic acini and islets, which showed pancreatic β-cell damage and degeneration with asymmetrical vacuoles. EFx treated STZ induced-DM rats showed marked improvement of the cellular injure as (Figure 9), as evident from the partial restoration of islet cells, reduced β-cell damage, more symmetrical vacuoles and an increase in number of islet cells.Figure 8

Bottom Line: Biochemical investigations were carried out according to previously reported methods.Histopathological examinations were done accordingly.Activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH) were significantly increased (p < 0.001) among EFx treated rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences (SHIATS)-Deemed University, Allahabad, India.

ABSTRACT

Background: Ethanolic extract of Euryale ferox salisb. (EFx) may have an effect on the activity of hepatic antioxidant enzymes, glycemic control and lipid profile and histopathology of pancreas, liver and kidney of streptozotocin (STZ)-induced diabetic wistar rats.

Methods: Wistar albino rats were divided into eight groups viz. non-diabetic (normal control), diabetic control (STZ-induced), diabetic treated (infused with different doses of Euryale ferox. Salisb. ethanolic extract) and diabetic conventional treated (treated with Glibenclamide). Diabetes was induced by administering streptozotocin (60 mg/kg body weight) intraperitoneal (i.p). The ethanolic extract was supplemented in different doses through oral route. Biochemical investigations were carried out according to previously reported methods. Histopathological examinations were done accordingly.

Results: The EFx supplemented diabetic rats significantly (p < 0.001) decreased the blood glucose level in a dose dependent manner. Plasma insulin level was significantly increased in EFx treated rats. The hepatic gluconeogenic enzymes activities were restored to normal in EFx treated rats. Activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH) were significantly increased (p < 0.001) among EFx treated rats. Lipid profile was reinstated to nearly normal level among EFx treated rats. Histopathological investigations revealed that microscopic architecture of pancreatic, hepatic and renal cells improvised in EFx treated diabetic rats.

Conclusion: EFx supplement could improve the glycemic control as well as lipid profile in diabetic rats along with improvised antioxidant enzymes which has beneficial effect in preventing the diabetic complications by scavenging the free radicals in diabetic rats.

No MeSH data available.


Related in: MedlinePlus